scholarly journals Apparent Inhibitory Effect of Fermented Brown Rice on Salivary Amylase Activity Relative to That of Acarbose, Guava Leaf Tea and Nucleic Acid

2013 ◽  
Vol 66 (3) ◽  
pp. 141-145
Author(s):  
Arinobu Yamauchi
Keyword(s):  
Nucleic Acid ◽  
Amylase Activity ◽  
Brown Rice ◽  
Inhibitory Effect ◽  
Salivary Amylase ◽  
Guava Leaf

scholarly journals Inhibitory effect of high leucine concentration on α-amylase secretion by pancreatic acinar cells: possible key factor of proteasome

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Long Guo ◽  
Baolong Liu ◽  
Chen Zheng ◽  
Hanxun Bai ◽  
Hao Ren ◽  
...  
Keyword(s):  
Amylase Activity ◽  
Acinar Cells ◽  
Inhibitory Effect ◽  
Proteasome Activity ◽  
Pancreatic Acinar Cells ◽  
Amylase Secretion ◽  
High Concentration ◽  
Amylase Release ◽  
Cholecystokinin Receptor ◽  
Key Factor

The present study aimed to investigate whether leucine affects the pancreatic exocrine by controlling the antisecretory factor (AF) and cholecystokinin receptor (CCKR) expression as well as the proteasome activity in pancreatic acinar cells of dairy calves. The pancreatic acinar cells were isolated from newborn Holstein bull calves and cultured using the Dulbecco’s modified Eagle’s medium/nutrient mixture F12 Ham’s liquid (DMEM/F12). There were six treatments of leucine dosage including 0 (control), 0.23, 0.45, 1.35, 4.05, and 12.15 mM, respectively. After culture for 3 h, the samples were collected for subsequent analysis. As the leucine concentration increased from 0 to 1.35 mM, the α-amylase activity in media decreased significantly (P<0.05), while further increase in leucine concentration did not show any decrease in α-amylase activity. Addition of leucine inhibited (P<0.05) the expression of AF and CCKR, and decreased the activity of proteasome (P<0.05) by 76%, 63%, 24%, 7%, and 9%, respectively. Correlation analysis results showed α-amylase secretion was negatively correlated with leucine concentration (P<0.01), and positively correlated with proteasome activity (P<0.01) and the expression of CCK1R (P<0.01) and AF (P<0.05). The biggest regression coefficient was showed between α-amylase activity and proteasome (0.7699, P<0.001). After inhibition of proteasome by MG-132, low dosage leucine decreased (P<0.05) the activity of proteasome and α-amylase, as well as the expression of CCK1R. In conclusion, we demonstrated that the high-concentration leucine induced decrease in α-amylase release was mainly by decreasing proteasome activity.

scholarly journals Study on rapid screening technology of antisense oligonucleotides for SARS-CoV-2

2021 ◽  
Author(s):  
Huawei Shen
Keyword(s):  
Nucleic Acid ◽  
Antisense Oligonucleotides ◽  
Structural Characteristics ◽  
Inhibitory Effect ◽  
Rapid Screening ◽  
Short Term ◽  
Physical Isolation ◽  
The World ◽  
Actual Use ◽  
Screening Technology

Abstract According to the structural characteristics of SARS-CoV-2 nucleic acid,SARS-CoV-2 genomes were virtually segmented.After comparing with human genome,44707 SARS-CoV-2 genomes and 26 primates' genomes, 19 antisense oligonucleotides sequences were selected.Experimental results show that the combined inhibitory effect reaches 100% . Significance statement：Currently, effective methods of controlling the spread of SARS-CoV-2 are very limited, only physical isolation and vaccination.Although physical isolation can achieve some short-term results, but can not fundamentally solve the problem of epidemic spread.The time between the development of a vaccine and its actual use is very long.It often happens that the vaccine is developed successfully, but the epidemic situation is beyond control.If a mutant strain emerges, it will take longer.I hope that the rapid screening technology of SARS-CoV-2 antisense oligonucleotides will light up new hope for the future of mankind and contribute oriental wisdom to the world.

scholarly journals Effect of kinetin on nucleic acid synthesis in senescing and young leaves in Brassica oleracea L. var. gongylodes L.

2015 ◽  
Vol 44 (4) ◽  
pp. 553-565
Author(s):  
J. Legocka ◽  
A. Szweykowska
Keyword(s):  
Molecular Weight ◽  
Nucleic Acid ◽  
Acid Synthesis ◽  
Inhibitory Effect ◽  
Rrna Synthesis ◽  
Low Molecular Weight ◽  
Chlorophyll B ◽  
Mature Leaves ◽  
Young Leaves ◽  
Brassica Oleracea L

In detached kohlrabi leaves senescing in the dark, the decrease in chlorophyll to was more pronounced than in chlorophyll a. The retardation by kinetin of the chlorophyll loss was also markedly stronger in the case of chlorophyll b. Using the fractionation of nucleic acids on polyacrylamide gels it has been shown that during leaf senescence the level of all RNA species decreased, whereas the amount of DNA was more or less constant. In the presence of kinetin, the loss of RNA was inhibited and the incorporation of precursor into the cytoplasmic rRNA as well as into low molecular weight RNA species was supported. Chloroplast rRNA synthesis has not been detected in mature leaves and kinetin showed no effect in this respect. In young expanding leaves detached and kept in light, the synthesis of cytoplasmic rRNA was strongly stimulated by kinetin, whereas in the case of Chloroplast rRNA only an inhibitory effect of kinetin could be found. The results suggest that the cytokinins are primarily involved in processes of the synthesis of cytoplasmic rRNA and low molecular RNA fractions, and in this way affect the development of plastids, in particular the course of their senescence.

scholarly journals Inhibitory effect of a weight-loss Chinese herbal formula RCM-107 on pancreatic α-amylase activity: Enzymatic and in silico approaches

PLoS ONE ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. e0231815 ◽  
Author(s):  
Shiqi Luo ◽  
George Binh Lenon ◽  
Harsharn Gill ◽  
Andrew Hung ◽  
Daniel A. Dias ◽  
...  
Keyword(s):  
Weight Loss ◽  
In Silico ◽  
Amylase Activity ◽  
Inhibitory Effect ◽  
Herbal Formula ◽  
Chinese Herbal Formula ◽  
Chinese Herbal

Rapid quantitative, specific measurement of pancreatic amylase in serum with use of a monoclonal antibody.

1985 ◽  
Vol 31 (8) ◽  
pp. 1283-1286 ◽  
Author(s):  
T E Mifflin ◽  
D C Benjamin ◽  
D E Bruns
Keyword(s):  
Polyvinylidene Fluoride ◽  
Amylase Activity ◽  
Kinetic Method ◽  
Reference Intervals ◽  
Pancreatic Amylase ◽  
Salivary Amylase ◽  
Electrophoretic Method ◽  
Standard Curve ◽  
A Value ◽  
Analytical Recovery

Abstract In this rapid quantitative assay for pancreatic alpha-amylase (EC 3.2.1.1) in serum, we precipitate salivary amylases by 10-min incubation with monoclonal anti-salivary amylase antibody immobilized on particles of polyvinylidene fluoride. We then centrifuge the serum mixture and measure the pancreatic amylase activity remaining in the supernate by a kinetic method. The assay requires 50 microL of serum and the standard curve is linear to at least 1300 U of pancreatic amylase per liter of serum. CVs were 1.3% within-run, 6-8% day-to-day. Apparent analytical recovery of pancreatic amylase activity added to serum was 101% +/- 2%. Addition of purified salivary amylase, 356 U/L, to sera gave a value for apparent pancreatic amylase of less than 4 U/L, or 1% of the added salivary amylase activity. This assay correlated well with an electrophoretic method (slope, 0.97-0.99; intercept, 0.5 to -4 U/L; correlation coefficient, 0.946-0.990; and standard error of the estimate 3-5 U/L). Estimated normal reference intervals with maltotetraose as substrate were: total amylase, 39-118 U/L; pancreatic amylase, 11-50 U/L; and salivary amylase, 18-79 U/L.

Effect of diet in West Africa on human salivary amylase activity

1972 ◽  
Vol 17 (10) ◽  
pp. 1415-1420 ◽  
Author(s):  
B. Wesley-Hadzija ◽  
H. Pigon
Keyword(s):  
West Africa ◽  
Amylase Activity ◽  
Salivary Amylase
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