scholarly journals Possible effects of the Agaricus brasiliensis extract on the cardiac morphology in mice

2015 ◽  
Vol 32 (01) ◽  
pp. 016-021
Author(s):  
E. Lessna ◽  
L. Bruning ◽  
C. Bolina ◽  
D. Lima ◽  
I. Watanabe ◽  
...  
Keyword(s):  
Structural Characteristics ◽  
Muscle Fibers ◽  
Polarized Light ◽  
Microscopic Analysis ◽  
Collagen Type I ◽  
Collagen Fibers ◽  
Herbal Remedies ◽  
Type I ◽  
Agaricus Brasiliensis ◽  
Swiss Mice

Abstract Introduction: Mushroom extracts are used, mainly due to immunomodulatory and antitumor proprierties. Among the general and species is the Agaricus brasiliensis or SunMushroom. However herbal remedies can cause changes in morphology and functionality of various organs. Therefore, these reporters need to be analyzed for applicability of this compound. The aim of the present study was to analyze the structural characteristics of the heart of Swiss mice employing light microscopy and scanning electron microscopy methods. Materials and methods: Swiss mice were used, divided into 2 groups: Control (n=6) and Agaricus brasiliensis (n=6), and the second group treated with the extract daily. After 60 days, under anesthesia withdrew from the samples, which were submitted to histological routine for obtaining serial sections. Results: Microscopic analysis revealed the aspects of the heart of Swiss mice: The formation of layers (epicardium, myocardium and endocardium) with partial view of the papillary muscles. Using the technique of Picro-Sirius: In yellow, the muscle fibers; in red, collagen fibers. Under polarized light, show the types of collagen fibers in red and green, significant collagen type I and III respectively. Conclusion: The arrangement of muscle fibers and collagen did not vary between the two groups.

scholarly journals Meniscal allograft transplantation in rabbit

2015 ◽  
Vol 87 (4) ◽  
pp. 2205-2216
Author(s):  
LIANA M. VILELA ◽  
RICARDO J. DEL CARLO ◽  
EDSON V. Melo FILHO ◽  
LUKIYA S.C. FAVARATO ◽  
TATIANA S. DUARTE ◽  
...  
Keyword(s):  
Collagen Type I ◽  
Collagen Fibers ◽  
The Body ◽  
Type I ◽  
Meniscal Allograft Transplantation ◽  
Allograft Transplantation ◽  
Meniscal Allograft ◽  
Preservation Method ◽  
Transplantation Technique ◽  
Partial Fixation

This study evaluated the technique for meniscal allograft transplantation using allografts preserved in glycerin 98% in rabbits. Euthanasia was performed at 70 days to compare the transplanted (TM1 to TM16) versus the contralateral meniscus (OM1 to OM16). Sixteen menisci, 8 transplanted and 8 contralateral, were submitted to gross examination, histomorphometric analysis for identification and quantification of cellular type, and for quantification and distribution of collagen fibers. A revascularization study was conducted in all of the other samples. Lengths of the OM varied from 0.9 to 1.0 cm and two TM were smaller. All TM were completely attached to the synovial membrane, except for one case that presented partial fixation. Both, TM and OM had similar amounts of chondrocytes, fibroblasts and fibrocytes, and at the horns, chondrocytes were predominant. The collagen fibers in TM were well organized throughout the body, and disorganized at the horns. These fibers in OM were organized. The amounts of collagen type I and III, and the vascularization of the perimeniscal tissue and of the edge were similar in OM and TM. These results demonstrated graft integration and thus this transplantation technique and preservation method may be recommended.

scholarly journals A Magneto-Microfluidic System for Investigating the Influence of an Externally Induced Force Gradient in a Collagen Type I ECM on HMVEC Sprouting

2016 ◽  
Vol 22 (4) ◽  
pp. 413-424 ◽  
Author(s):  
Sahan C. B. Herath ◽  
Soheila Sharghi-Namini ◽  
Yue Du ◽  
Dongan Wang ◽  
Ruowen Ge ◽  
...  
Keyword(s):  
Magnetic Beads ◽  
Collagen Type ◽  
Collagen Type I ◽  
Microfluidic System ◽  
Collagen Fibers ◽  
Cell Behavior ◽  
Type I ◽  
Pathological Angiogenesis ◽  
A Cell

Advances in mechanobiology have suggested that physiological and pathological angiogenesis may be differentiated based on the ways in which the cells interact with the extracellular matrix (ECM) that exhibits partially different mechanical properties. This warrants investigating the regulation of ECM stiffness on cell behavior using angiogenesis assays. In this article, we report the application of the technique of active manipulation of ECM stiffness to study in vitro angiogenic sprouting of human microvascular endothelial cells (HMVECs) in a microfluidic device. Magnetic beads were embedded in the ECM through bioconjugation (between the streptavidin-coated beads and collagen fibers) in order to create a pretension in the ECM when under the influence of an external magnetic field. The advantage of using this magneto-microfluidic system is that the resulting change in the local deformability of the collagen fibers is only apparent to a cell at the pericellular level near the site of an embedded bead, while the global intrinsic material properties of the ECM remain unchanged. The results demonstrate that this system represents an effective tool for inducing noninvasively an external force on cells through the ECM, and suggest the possibility of creating desired stiffness gradients in the ECM for manipulating cell behavior in vitro.

scholarly journals Skin biopsy as a method for determining indications for preventive endoprosthetics of the anterior abdominal wall

2020 ◽  
pp. 46-53
Author(s):  
Viktor A. Lazarenko ◽  
Sergey V. Ivanov ◽  
Ilya S. Ivanov ◽  
Evgeny G. Ob’edkov ◽  
Nikolay N. Grigor'ev ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Skin Biopsy ◽  
Abdominal Wall ◽  
Anterior Abdominal Wall ◽  
Polarized Light ◽  
Structural Features ◽  
Collagen Fibers ◽  
Type Iii Collagen ◽  
Type I ◽  
Connective Tissue Dysplasia

The aim is to examine the effectiveness of preoperative skin biopsy method to determine indications for preventive endoprosthesis implantation of the anterior abdominal wall based on structural features of connective tissue. Materials and methods. The study included 17 patients who had been planned to undergo a scheduled surgery such as video laparoscopic cholecystectomy. The histological material was collected with DERMO PUNCH biopsy needles in an outpatient clinic. Using polarized light microscopy we inspected histological slices coloured with Sirius red. After that we analyzed histological sections including the ratio of collagen fibers, the density of connective tissue and the number of interfibrous spaces. Results. All the studied features of connective tissue dysplasia had been registered among 18% of patients; significant differences (p ≤0.05) had been identified among other 24% of patients. In patients with identified signs of connective tissue dysplasia, an increase in type III collagen fibers and a decrease in type I fibers were revealed, which directly resulted in a decrease in their ratio. In addition, this category of patients noted a decrease in the density of connective tissue and a significant number of interfibrous spaces. Conclusion. Skin biopsy is a safe and informative method to reveal patients with predisposition to the formation of hernias of the anterior abdominal wall based on structural features of connective tissue and to determine indications for preventive endoprosthetic implantation. This method will reduce the frequency of postoperative ventral hernias, so the effectiveness of the treatment will definitely increase.

scholarly journals Collagen Characterization in a Model of Nonalcoholic Steatohepatitis with Fibrosis; a Call for Development of Targeted Therapeutics

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3316
Author(s):  
Anthony J. Pellicano ◽  
Kiera Spahn ◽  
Ping Zhou ◽  
Itzhak D. Goldberg ◽  
Prakash Narayan
Keyword(s):  
Liver Disease ◽  
Nonalcoholic Steatohepatitis ◽  
Collagen Type ◽  
Microscopic Analysis ◽  
Collagen Type I ◽  
Hepatic Tissue ◽  
Type I ◽  
Nonalcoholic Fatty Liver ◽  
End Stage ◽  
Modified Diet

Left untreated, nonalcoholic fatty liver disease can progress to nonalcoholic steatohepatitis (NASH), fibrosis, and end-stage liver disease. To date, few if any therapies have proven effective against NASH with fibrosis. Quantification and qualification of hepatic scar might enable development of more effective targeted therapies. In a murine model of NASH induced by diet, we characterized fibrillar collagen deposition within the hepatic parenchyma. At harvest, livers from the modified diet cohort exhibited NASH with fibrosis. Transcriptomic analysis of hepatic tissue revealed increased col1a1, col1a2, and col3a1, each of which correlated directly with hepatic hydroxyproline content. Circular polarized microscopic analysis of Picrosirius red-stained liver sections revealed deposition of collagen type I within the parenchyma. Development of therapeutics designed to mitigate collagen type I accumulation might prove effective in NASH with fibrosis.

scholarly journals Orientation Matters: Polarization Dependent IR Spectroscopy of Collagen from Intact Tendon Down to the Single Fibril Level

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4295
Author(s):  
Gorkem Bakir ◽  
Benoit E. Girouard ◽  
Richard Wiens ◽  
Stefan Mastel ◽  
Eoghan Dillon ◽  
...  
Keyword(s):  
Ir Spectroscopy ◽  
Near Field ◽  
Polarized Light ◽  
Scar Tissue ◽  
Collagen Type I ◽  
Array Detector ◽  
Type I ◽  
Absorption Bands ◽  
Spectral Profiles ◽  
Ir Light

Infrared (IR) spectroscopy has been used for decades to study collagen in mammalian tissues. While many changes in the spectral profiles appear under polarized IR light, the absorption bands are naturally broad because of tissue heterogeneity. A better understanding of the spectra of ordered collagen will aid in the evaluation of disorder in damaged collagen and in scar tissue. To that end, collagen spectra have been acquired with polarized far-field (FF) Fourier Transform Infrared (FTIR) imaging with a Focal Plane Array detector, with the relatively new method of FF optical photothermal IR (O-PTIR), and with nano-FTIR spectroscopy based on scattering-type scanning near-field optical microscopy (s-SNOM). The FF methods were applied to sections of intact tendon with fibers aligned parallel and perpendicular to the polarized light. The O-PTIR and nano-FTIR methods were applied to individual fibrils of 100–500 nm diameter, yielding the first confirmatory and complementary results on a biopolymer. We observed that the Amide I and II bands from the fibrils were narrower than those from the intact tendon, and that both relative intensities and band shapes were altered. These spectra represent reliable profiles for normal collagen type I fibrils of this dimension, under polarized IR light, and can serve as a benchmark for the study of collagenous tissues.

Methionine-Specific Staining of Collagen

1982 ◽  
Vol 40 ◽  
pp. 294-295
Author(s):  
E.M. Kuhn ◽  
K.D. Marenus ◽  
M. Beer
Keyword(s):  
Amino Acids ◽  
Collagen Fibers ◽  
Type Iii Collagen ◽  
Type I ◽  
Electron Microscopic ◽  
Good Correspondence ◽  
Specific Staining ◽  
Type Iii ◽  
Different Types ◽  
Sulfur Containing

Fibers composed of different types of collagen cannot be differentiated by conventional electron microscopic stains. We are developing staining procedures aimed at identifying collagen fibers of different types.Pt(Gly-L-Met)Cl binds specifically to sulfur-containing amino acids. Different collagens have methionine (met) residues at somewhat different positions. A good correspondence has been reported between known met positions and Pt(GLM) bands in rat Type I SLS (collagen aggregates in which molecules lie adjacent to each other in exact register). We have confirmed this relationship in Type III collagen SLS (Fig. 1).

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