scholarly journals Regeneration of periodontal bone defects with dental pulp stem cells grafting: Systematic Review

2019 ◽  
pp. 0-0 ◽  
Author(s):  
S Amghar-Maach ◽  
C Gay-Escoda ◽  
MÁ Sanchez-Garces
Keyword(s):  
Systematic Review ◽  
Stem Cells ◽  
Dental Pulp ◽  
Bone Defects ◽  
Dental Pulp Stem Cells ◽  
Periodontal Bone Defects

scholarly journals Fibrin scaffolds containing dental pulp stem cells for the repair of periodontal bone defects

2020 ◽  
Vol 7 (1) ◽  
pp. 59-69
Author(s):  
Yu. A. Dombrovskaya ◽  
N. I. Enukashvily ◽  
A. V. Kotova ◽  
S. S. Bilyk ◽  
A. N. Kovalenko ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Tissue ◽  
Fibrin Glue ◽  
Dental Pulp ◽  
Bone Defects ◽  
Dental Pulp Stem Cells ◽  
Osteogenic Potential ◽  
Tooth Pulp ◽  
Alizarin Red ◽  
Periodontal Bone Defects

Background. 3D scaffolds plays an important role in developing new approaches in modern dentistry. They are used to establish optimal conditions for cell differentiation, vascularization and remodeling of regenerating bone tissue.Objective. Evaluation of the possibility of creating scaffolds developed on the basis of 3D modeling of periodontal bone defects and containing tooth pulp stem cells.Materials and Methods. The computer tomography data of the maxillar bone tissue defect were analysed. Anatomical prototype — a mold representing defects of the vestibular and palatal fragments of bone tissue was created by 3D printing. This 3D form was filled with fibrin glue and dental pulp stem cells. The fibrin glue was prepared from autologous blood plasma and mixed with dental pulp stem cells.Results. Fibrin glue prepared from an autologous plasma concentrate (fibrinogen 20 g/l) retains its shape for 4 days. On the day 5, the clot retraction became clearly visible and on the day 7, the clot diameter decreased to 50 % of the original size. The proliferation rate of cells, grown both inside the scaffold and in 2D conditions, did not differ. The immunophenotype of cells of both groups corresponded to the immunophenotype of mesenchy mal stromal cells. The mesenchymal immunophenotype is a feature of dental stem cells. Alizarin red staining of cells both grown on adhesive culture plastic and extracted from glue on day 10 after the induction of osteogenic differentiation did not differ.Conclusion. The fibrin glue is a good material for creation a scaffold with suitable mechanical characteristics. The cells enclosed in the fibrin glue maintain their viability, immunophenotype and osteogenic potential. This technology can be used for bone tissue repair in dentistry and maxillofacial surgery.

Cartilage regeneration with human dental pulp stem cells - a systematic review

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S175-S178
Author(s):  
J. Santanna ◽  
T.L. Fernandez ◽  
D.F. Bueno ◽  
C.C. Pinheiro ◽  
A.J. Hernandez
Keyword(s):  
Systematic Review ◽  
Stem Cells ◽  
Dental Pulp ◽  
Cartilage Regeneration ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

Human Dental Pulp Stem Cells in Rat Mandibular Bone Defects

2019 ◽  
Vol 207 (3-4) ◽  
pp. 138-148 ◽  
Author(s):  
Rubia Teodoro Stuepp ◽  
Priscilla Barros Delben ◽  
Filipe Modolo ◽  
Andrea Gonçalves Trentin ◽  
Ricardo Castilho Garcez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Dental Pulp ◽  
Bone Defects ◽  
Treated Group ◽  
Dental Pulp Stem Cells ◽  
Control Group ◽  
Mandibular Bone ◽  
Significant Difference ◽  
Human Dental Pulp

This study aimed to evaluate the use of human dental pulp stem cells (hDPSCs) in non-critical-sized mandibular bone defects in rats. hDPSCs from permanent teeth were isolated and engrafted in mandibular bone defects in rats for 7, 14, and 28 days; bone defects without cells formed the control group. Samples were evaluated by scanning electron microscopy (SEM), light microscopy (hematoxylin and eosin staining), and the regeneration area was measured by the Image J program. Before surgery procedures, the human dental pulp cells were characterized as dental pulp stem cells: fusiform morphology, plastic-adherent; expression of CD105, CD73, and CD90; lack of expression of CD45 and CD34, and differentiated into osteoblasts, adipocytes, and chondroblasts. The results indicated that within 7 days the control group presented a pronounced bone formation when compared with the treated group (p < 0.05). After 14 days, the treated group showed an increase in bone formation, but with no statistical difference among the groups (p > 0.05). In the final evaluated period there was no difference between the control group and the treated group (p > 0.05). There was a significant difference between 7 and 14 days (p < 0.05) and between 7 and 28 days (p < 0.05) in the treated group. In conclusion, there is no evidence that the use of hDPSCs in the conditions of this study could improve bone formation in non-critical-sized mandibular bone defects.

scholarly journals Efficacy of extracellular vesicles from dental pulp stem cells for bone regeneration in rat calvarial bone defects

2021 ◽  
Vol 41 (1) ◽  
Author(s):  
Yuka Imanishi ◽  
Masaki Hata ◽  
Ryohei Matsukawa ◽  
Atsushi Aoyagi ◽  
Maiko Omi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Extracellular Vesicles ◽  
Dental Pulp ◽  
Bone Defects ◽  
Dental Pulp Stem Cells ◽  
The Body ◽  
Histological Observation ◽  
Micro Ct ◽  
Calvarial Bone

Abstract Background Extracellular vesicles (EVs) are known to be secreted by various cells. In particular, mesenchymal stem cell (MSC)-derived EVs (MSC-EVs) have tissue repair capacity and anti-inflammatory properties. Dental pulp stem cells (DPSCs), which are MSCs isolated from pulp tissue, are less invasive to the body than other MSCs and can be collected from young individuals. In this study, we investigated the efficacy of EVs secreted by DPSCs (DPSC-EVs) for bone formation. Methods DPSC-EVs were isolated from the cell culture medium of DPSCs. DPSC-EVs were unilaterally injected along with collagen (COL), beta-tricalcium phosphate (β-TCP) or hydroxyapatite (HA) into rat calvarial bone defects. The effects of DPSC-EVs were analyzed by micro-computed tomography (micro-CT) and histological observation. Results Micro-CT showed that administration of DPSC-EVs with the abovementioned scaffolds resulted in bone formation in the periphery of the defects. DPSC-EVs/COL specifically resulted in bone formation in the center of the defects. Histological observation revealed that DPSC-EVs/COL promoted new bone formation. Administration of DPSC-EVs/COL had almost the same effect on the bone defect site as transplantation of DPSCs/COL. Conclusions These results suggest that DPSC-EVs may be effective tools for bone tissue regeneration.

scholarly journals Present Status of Research on the Regenerative Potential of Dental Pulp Stem Cells in Malaysia: A Systematic Review

2021 ◽  
Vol 8 (1) ◽  
pp. 304-309
Author(s):  
Nazmul Haque
Keyword(s):  
Systematic Review ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Present Status ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Invasive Technique ◽  
Human Exfoliated Deciduous Teeth

Stem cells from human exfoliated deciduous teeth (SHED) or dental pulp stem cells (DPSCs) from permanent teeth are considered promising sources of mesenchymal stem cells. It requires a less invasive technique to isolate stem cells from exfoliated or permanent teeth. Hence this study aimed to identify the present status of research on the regenerative potential of SHED/DPSCs in Malaysia. The results indicate that only 60 articles were published in regenerative medicine from Malaysia till 5th July 2019. Only 16 tertiary institutes and four industries/clinics were involved in these studies. A poor pattern of collaboration has also been identified. Outcomes of this study have emphasized the conduction of more research on the regenerative potential of SHED/DPSCs, and active collaboration among the tertiary institutes and industries for successful translation of these cells from bench side to bedside.

scholarly journals The use of human dental pulp stem cells for in vivo bone tissue engineering: A systematic review

2018 ◽  
Vol 9 ◽  
pp. 204173141775276 ◽  
Author(s):  
Alessander Leyendecker Junior ◽  
Carla Cristina Gomes Pinheiro ◽  
Tiago Lazzaretti Fernandes ◽  
Daniela Franco Bueno
Keyword(s):  
Systematic Review ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Deciduous Teeth ◽  
Human Dental Pulp

Dental pulp represents a promising and easily accessible source of mesenchymal stem cells for clinical applications. Many studies have investigated the use of human dental pulp stem cells and stem cells isolated from the dental pulp of human exfoliated deciduous teeth for bone tissue engineering in vivo. However, the type of scaffold used to support the proliferation and differentiation of dental stem cells, the animal model, the type of bone defect created, and the methods for evaluation of results were extremely heterogeneous among these studies conducted. With this issue in mind, the main objective of this study is to present and summarize, through a systematic review of the literature, in vivo studies in which the efficacy of human dental pulp stem cells and stem cells from human exfoliated deciduous teeth (SHED) for bone regeneration was evaluated. The article search was conducted in PubMed/MEDLINE and Web of Science databases. Original research articles assessing potential of human dental pulp stem cells and SHED for in vivo bone tissue engineering, published from 1984 to November 2017, were selected and evaluated in this review according to the following eligibility criteria: published in English, assessing dental stem cells of human origin and evaluating in vivo bone tissue formation in animal models or in humans. From the initial 1576 potentially relevant articles identified, 128 were excluded due to the fact that they were duplicates and 1392 were considered ineligible as they did not meet the inclusion criteria. As a result, 56 articles remained and were fully analyzed in this systematic review. The results obtained in this systematic review open new avenues to perform bone tissue engineering for patients with bone defects and emphasize the importance of using human dental pulp stem cells and SHED to repair actual bone defects in an appropriate animal model.

Systematic Review of Human Dental Pulp Stem Cells for Cartilage Regeneration

2020 ◽  
Vol 26 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Tiago Lazzaretti Fernandes ◽  
João Paulo Cortez de SantAnna ◽  
Igor Frisene ◽  
João Paulo Gazarini ◽  
Carla Cristina Gomes Pinheiro ◽  
...  
Keyword(s):  
Systematic Review ◽  
Stem Cells ◽  
Dental Pulp ◽  
Cartilage Regeneration ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

Strontium folate loaded biohybrid scaffolds seeded with dental pulp stem cells induce in vivo bone regeneration in critical sized defects

2016 ◽  
Vol 4 (11) ◽  
pp. 1596-1604 ◽  
Author(s):  
Marcela Martin-del-Campo ◽  
Raul Rosales-Ibañez ◽  
Keila Alvarado ◽  
Jose G. Sampedro ◽  
Christian A. Garcia-Sepulveda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Dental Pulp ◽  
Bone Defects ◽  
Dental Pulp Stem Cells ◽  
Critical Bone Defects ◽  
Complete Regeneration

Strontium folate loaded biohybrid scaffolds enhance dental pulp stem cells replication and differentiation, promoting complete regeneration of critical bone defects.

scholarly journals Regenerative capacity of dental pulp stem cells: A systematic review

2020 ◽  
Vol 12 (5) ◽  
pp. 27
Author(s):  
GururajNarayana Rao ◽  
AdlinS Rosaian ◽  
SunilP Mohan ◽  
Mahalakshmi Vijayarajan ◽  
RebekkahC Prabhakaran ◽  
...  
Keyword(s):  
Systematic Review ◽  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Regenerative Capacity
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