scholarly journals A swift, easy and cheap protocol to evaluate the tooth bleaching in vitro

Author(s):  
KVF Penha ◽  
ACS Sousa ◽  
CA Oliveira ◽  
RSB de Andrade ◽  
DFP Vasconcelos
Keyword(s):  
2006 ◽  
Vol 7 (1) ◽  
pp. 25-34 ◽  
Author(s):  
Luís Roberto Marcondes Martins ◽  
Claudia Cia Worschech ◽  
José Augusto Rodrigues ◽  
Gláucia Maria Bovi Ambrosano

Abstract During tooth bleaching abrasive dentifrices might change the outer superficial enamel. The aim of this in vitro study was to evaluate the roughness of human enamel exposed to a 10% carbamide peroxide bleaching agent at different times and submitted to different superficial cleaning treatments. The study consisted of 60 sound human enamel slabs, randomly assigned to different treatment groups: G1 - not brushed; G2 - brushed with a fluoride abrasive dentifrice; G3 - brushed with a non-fluoride abrasive dentifrice; and G4 - brushed without a dentifrice. There were 15 enamel slabs per group. Slabs of molar teeth were obtained and sequentially polished with sandpaper and abrasive pastes. A perfilometer was used to obtain the mean of Ra value on the surface of each specimen to initial and experimental times. Bleaching was performed on the enamel surface for six hours daily. After that, each slab received a cleaning surface treatment and was stored in artificial saliva. Analysis of variance (ANOVA) and Tukey's HSD hoc analysis (α =0.05) revealed significant differences in roughness values over time for enamel bleached and treated with different superficial cleaning methods. G1 and G4 showed no significant differences in roughness over time, G2 and G3 showed a significant increase in the surface roughness values. This in vitro investigation showed the sole use of 10% carbamide peroxide did not alter the enamel surface roughness, but the cleaning treatments that employed the use of brushing with abrasive dentifrices resulted in a significant increase of enamel surface roughness. Citation Worschech CC, Rodrigues JA, Martins LRM, Ambrosano GMB. Brushing Effect of Abrasive Dentifrices during At-home Bleaching with 10% Carbamide Peroxide on Enamel Surface Roughness. J Contemp Dent Pract 2006 February;(7)1:025-034.


Author(s):  
Helena Maria Gorgulho Pitacas ◽  
Alexandre Cavalheiro ◽  
Catarina Coito ◽  
Ana Silva ◽  
Raquel Eira ◽  
...  

2008 ◽  
Vol 24 (1) ◽  
pp. 57-66 ◽  
Author(s):  
Bor-Shiunn Lee ◽  
Shih-Hao Huang ◽  
Yu-Chih Chiang ◽  
Yu-Shan Chien ◽  
Chung-Yuan Mou ◽  
...  
Keyword(s):  

2019 ◽  
Vol 98 (4) ◽  
pp. 443-449
Author(s):  
O. Redha ◽  
A. Strange ◽  
A. Maeva ◽  
R. Sambrook ◽  
N. Mordan ◽  
...  

Carbamide peroxide (CP) is widely used as a tooth-whitening agent in self-administered tooth-bleaching products. In this study, the effects of 5% and 10% CP on dentinal collagen structure and chemical properties were evaluated in vitro. Thirty-five intact teeth were exposed to 2 whitening protocols (2 or 4 h daily) with either 5% or 10% CP gel for 1 wk. Shade changes before and after the whitening protocol were captured colorimetrically using a spectroshade. Collagen scaffold models and demineralized dentine disc samples were prepared and exposed to CP droplets (5% or 10%). Structural changes were investigated using electron microscopy. Finally, mineralized dentine disc samples were prepared postbleaching to assess chemical changes resulting from CP exposure in dentinal collagen using Raman spectroscopy. Results showed a difference in tooth shade when exposed to 5% and 10% CP whitening protocols, with a significantly ( P ≤ 0.01) greater change reported for the 10% CP/4-h group. Imaging of the collagen scaffold model following exposure to CP showed a gelatinization process indicating that the free radical by-products from CP are able to disrupt the quaternary structure of noncrosslinked collagen. The most significant damage on the collagen scaffold was seen for the 10% CP exposure for 4 h. Imaging of the demineralized discs displayed the same glassy amorphous layer appearance as found in the collagen scaffold. Raman spectra of the mineralized dentine discs showed a significant decrease ( P ≤ 0.01) in the integrated area of amide I and amide III values in the 4 test groups following CP application. Amide I was more affected as both the exposure time and concentration of CP increased. Despite the claimed safety of whitening agents, this in vitro study concludes that even low concentrations of CP result in a deleterious change in dentinal collagen.


2011 ◽  
Vol 35 (3) ◽  
pp. 289-294 ◽  
Author(s):  
Divya Sharma ◽  
Sanjay Sharma ◽  
SM Natu ◽  
Satish Chandra

Objectives: External root resorption is the complication of intra-coronal bleaching done with 30% H2O2alone or mixed with sodium perborate but not with sodium perborate mixed with water. The study was done to comparatively evaluate the H2O2 leakage from three H2O2 liberating bleaching agents. Study design:Fifty one single rooted human teeth were used. After root canal therapy gutta percha was removed below cemento-enamel junction. Three bleaching agents: sodium perborate mixed with water , sodium perborate mixed with 30% H2O2 and 30% H2O2 alone were used. Teeth without defect , with cervical root defect and with mid root defect constituted group A, group B and group C. According to various bleaching agents groups were subdivided into subgroup 1, 2 and 3. H2O2 leakage was measured with the help of spectrophotometer. Results: Almost all teeth showed H2O2 leakage. It was maximum in B1 followed by C1, B2,A1, A2, C2, B3, A3 and C3. Conclusion: Sodium perborate mixed with water was found to be the best bleaching agent.


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