scholarly journals AF3p21 (ALL1 fused gene from chromosome 3p21)

2011 ◽  
Author(s):  
JL Huret
Keyword(s):  
Fused Gene ◽  
Chromosome 3P21

scholarly journals Evidence for Selection at thefusedLocus ofDrosophila virilis

Genetics ◽  
2000 ◽  
Vol 155 (4) ◽  
pp. 1701-1709 ◽  
Author(s):  
Jorge Vieira ◽  
Brian Charlesworth
Keyword(s):  
Amino Acid ◽  
Aspartic Acid ◽  
Kinase Domain ◽  
Drosophila Virilis ◽  
Serine Threonine Kinase ◽  
Threonine Kinase ◽  
Usual Equilibrium ◽  
Replacement Site ◽  
Fused Gene ◽  
Significant Linkage

AbstractThe genomic DNA sequence of a 2.4-kb region of the X-linked developmental gene fused was determined in 15 Drosophila virilis strains. One common replacement polymorphism is observed, where a negatively charged aspartic amino acid is replaced by the noncharged amino acid alanine. This replacement variant is located within the serine/threonine kinase domain of the fused gene and is present in ~50% of the sequences in our sample. Significant linkage disequilibrium is detected around this replacement site, although the fused gene is located in a region of the D. virilis X chromosome that seems to experience normal levels of recombination. In a 600-bp region around the replacement site, all eight alanine sequences are identical; of the six aspartic acid sequences, three are also identical. The occurrence of little or no variation within the aspartic acid and alanine haplotypes, coupled with the presence of several differences between them, is very unlikely under the usual equilibrium neutral model. Our results suggest that the fused alanine haplotypes have recently increased in frequency in the D. virilis population.

Suggestive evidence for linkage to chromosome 3p21 in finnish inflammatory bowel disease families

2000 ◽  
Vol 118 (4) ◽  
pp. A336
Author(s):  
Paulina Paavola ◽  
Tiina Helio ◽  
Leena Halme ◽  
Ulla Turunen ◽  
Martti Farkkila ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Suggestive Evidence ◽  
Inflammatory Bowel ◽  
Chromosome 3P21

Identification of a regulatory region that mediates glucose-dependent induction of the Saccharomyces cerevisiae enolase gene ENO2

1986 ◽  
Vol 6 (7) ◽  
pp. 2287-2297
Author(s):  
R Cohen ◽  
J P Holland ◽  
T Yokoi ◽  
M J Holland
Keyword(s):  
Gene Expression ◽  
Regulatory Region ◽  
Carbon Sources ◽  
Initiation Site ◽  
Deletion Mapping ◽  
Regulatory Regions ◽  
Coding Sequences ◽  
Flanking Region ◽  
Fused Gene ◽  
In Cells

There are two yeast enolase genes, designated ENO1 and ENO2, which are expressed differentially in vegetative cells grown on glucose and in cells grown on gluconeogenic carbon sources. ENO2 is induced more than 20-fold in cells grown on glucose, whereas ENO1 expression is similar in cells grown on glucose and in cells grown on gluconeogenic carbon sources. Sequences within the 5' flanking region of ENO2 which are required for glucose-dependent induction were identified by deletion mapping analysis. These studies were carried out by using a fused gene containing the ENO2 5' flanking sequences and the ENO1 coding sequences. This fused gene undergoes glucose-dependent induction and is expressed at the same level as the resident ENO2 gene in cells grown on glucose or gluconeogenic carbon sources. Expression of fused genes containing deletion mutations within the ENO2 5' flanking region was monitored after integration at the ENO1 locus of a strain carrying a deletion of the resident ENO1 coding sequences. This analysis showed that there are two upstream activation sites located immediately upstream and downstream from a position 461 base pairs upstream from the transcriptional initiation site. Either one of these upstream activation sites is sufficient for glucose-dependent induction and normal gene expression in the presence of gluconeogenic carbon sources. Deletion of both regulatory regions results in a complete loss of gene expression. The regulatory regions function normally in both orientations relative to the coding sequences. Mutant fused genes containing small deletions within the regulatory regions were constructed; these genes were expressed normally in gluconeogenic carbon sources but were not induced in the presence of glucose. Based on this analysis, ENO2 contains a cis-acting regulatory region which is required for gene expression and mediates glucose-dependent induction of gene expression.

scholarly journals The 5' untranslated region of mRNA for ribosomal protein S19 is involved in its translational regulation during Xenopus development.

1990 ◽  
Vol 10 (2) ◽  
pp. 816-822 ◽  
Author(s):  
P Mariottini ◽  
F Amaldi
Keyword(s):  
Ribosomal Protein ◽  
Untranslated Region ◽  
Ribosomal Proteins ◽  
Translational Regulation ◽  
Xenopus Development ◽  
Untranslated Sequence ◽  
Gene Coding ◽  
Ribosomal Protein S19 ◽  
Fused Gene

During Xenopus development, the synthesis of ribosomal proteins is regulated at the translational level. To identify the region of the ribosomal protein mRNAs responsible for their typical translational behavior, we constructed a fused gene in which the upstream sequences (promoter) and the 5' untranslated sequence (first exon) of the gene coding for Xenopus ribosomal protein S19 were joined to the coding portion of the procaryotic chloramphenicol acetyltransferase (CAT) gene deleted of its own 5' untranslated region. This fused gene was introduced in vivo by microinjection into Xenopus fertilized eggs, and its activity was monitored during embryogenesis. By analyzing the pattern of appearance of CAT activity and the distribution of the S19-CAT mRNA between polysomes and messenger ribonucleoproteins, it was concluded that the 35-nucleotide-long 5' untranslated region of the S19 mRNA is able to confer to the fused S19-CAT mRNA the translational behavior typical of ribosomal proteins during Xenopus embryo development.

Possible role of tropomyosin-receptor kinase fused gene on skin collagen remodeling

2017 ◽  
Vol 88 (3) ◽  
pp. 375-377 ◽  
Author(s):  
Jung-Min Shin ◽  
So-Jung Kong ◽  
Young-Ah Shin ◽  
Yun-Hee Chang ◽  
Ji-Young Kim ◽  
...  
Keyword(s):  
Receptor Kinase ◽  
Collagen Remodeling ◽  
Skin Collagen ◽  
Fused Gene

scholarly journals Polycistronic transcription of fused cassettes and identification of translation initiation signals in an unusual gene cassette array from Pseudomonas aeruginosa

F1000Research ◽  
2015 ◽  
Vol 2 ◽  
pp. 99
Author(s):  
Érica L. Fonseca ◽  
Ana Carolina Paulo Vicente
Keyword(s):  
Real Time ◽  
Northern Blot ◽  
Gene Cassette ◽  
Rt Pcr ◽  
Recombination Site ◽  
Gene Cassettes ◽  
Polycistronic Transcription ◽  
Polymerase Chain ◽  
Fused Gene ◽  
Gene Cassette Array

The gene cassettes found in class 1 integrons are generally promoterless units composed by an open reading frame (ORF), a short 5’ untranslated region (UTR) and a 3’ recombination site (attC). Fused gene cassettes are generated by partial or total loss of the attC from the first cassette in an array, creating, in some cases, a fusion with the ORF from the next cassette. These structures are rare and little is known about their mechanisms of mobilization and expression. The aim of this study was to evaluate the dynamic of mobilization and transcription of the gcu14-blaGES-1/aacA4 gene cassette array, which harbours a fused gene cassette represented by blaGES-1/aacA4. The cassette array was analyzed by Northern blot and real-time reverse transcription-polymerase chain reaction (RT-PCR) in order to assess the transcription mechanism of blaGES-1/aacA4 fused cassette. Also, inverse polymerase chain reactions (PCR) were performed to detect the free circular forms of gcu14, blaGES-1 and aacA4. The Northern blot and real time RT-PCR revealed a polycistronic transcription, in which the fused cassette blaGES-1/aacA4 is transcribed as a unique gene, while gcu14 (with a canonical attC recombination site) has a monocistronic transcription. The gcu14 cassette, closer to the weak configuration of cassette promoter (PcW), had a higher transcription level than blaGES-1/aacA4, indicating that the cassette position affects the transcript amounts. The presence of ORF-11 at attI1, immediately preceding gcu14, and of a Shine-Dalgarno sequence upstream blaGES-1/aacA4 composes a scenario for the occurrence of array translation. Inverse PCR generated amplicons corresponding to gcu14, gcu14-aacA4 and gcu14-blaGES-1/aacA4 free circular forms, but not to blaGES-1 and aacA4 alone, indicating that the GES-1 truncated attC is not substrate of integrase activity and that these genes are mobilized together as a unique cassette. This study was original in showing the transcription of fused cassettes and in correlating cassette position with transcription.

scholarly journals Family-based association analysis validates chromosome 3p21 as a putative nasopharyngeal carcinoma susceptibility locus

2006 ◽  
Vol 8 (3) ◽  
pp. 156-160 ◽  
Author(s):  
Zhaoyang Zeng ◽  
Yanhong Zhou ◽  
Wenling Zhang ◽  
Xiaoling Li ◽  
Wei Xiong ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Association Analysis ◽  
Susceptibility Locus ◽  
Family Based ◽  
Chromosome 3P21

scholarly journals Genetic analysis in Finnish families with inflammatory bowel disease supports linkage to chromosome 3p21

2001 ◽  
Vol 9 (5) ◽  
pp. 328-334 ◽  
Author(s):  
Paulina Paavola ◽  
Tiina Heliö ◽  
Maija Kiuru ◽  
Leena Halme ◽  
Ulla Turunen ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Genetic Analysis ◽  
Bowel Disease ◽  
Inflammatory Bowel ◽  
Chromosome 3P21

scholarly journals Loss of Heterozygosity of Chromosome 3p21 Is Associated with Mutant TP53 and Better Patient Survival in Non–Small-Cell Lung Cancer

2004 ◽  
Vol 64 (23) ◽  
pp. 8702-8707 ◽  
Author(s):  
Carmen J. Marsit ◽  
Masayuki Hasegawa ◽  
Tomoko Hirao ◽  
Duk-Hwan Kim ◽  
Kenneth Aldape ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Loss Of Heterozygosity ◽  
Cell Lung Cancer ◽  
Patient Survival ◽  
Small Cell ◽  
Small Cell Lung ◽  
Chromosome 3P21
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