scholarly journals A rapid and sensitive loop-mediated isothermal amplification procedure (LAMP) for Mycoplasma hyopneumoniae detection based on the p36 gene

2015 ◽  
Vol 14 (2) ◽  
pp. 4677-4686 ◽  
Author(s):  
M.J. Liu ◽  
G.M. Du ◽  
F.F. Bai ◽  
Y.Z. Wu ◽  
Q.Y. Xiong ◽  
...  
Keyword(s):  
Mycoplasma Hyopneumoniae ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Procedure

scholarly journals Development of a Loop-Mediated Isothermal Amplification Procedure as a Sensitive and Rapid Method for Detection of ‘Candidatus Liberibacter solanacearum’ in Potatoes and Psyllids

2012 ◽  
Vol 102 (9) ◽  
pp. 899-907 ◽  
Author(s):  
Aravind Ravindran ◽  
Julien Levy ◽  
Elizabeth Pierson ◽  
Dennis C. Gross
Keyword(s):  
Bacterial Pathogen ◽  
Cost Effective ◽  
Isothermal Amplification ◽  
Lamp Method ◽  
Candidatus Liberibacter Solanacearum ◽  
Loop Mediated Isothermal Amplification ◽  
Potato Plants ◽  
Cost Effective Method ◽  
Candidatus Liberibacter ◽  
Amplification Procedure

This study reports the development of a loop-mediated isothermal amplification procedure (LAMP) for polymerase chain reaction (PCR)-based detection of ‘Candidatus Liberibacter solanacearum’, the bacterial causal agent of potato zebra chip (ZC) disease. The 16S rDNA gene of ‘Ca. Liberibacter solanacearum’ was used to design a set of six primers for LAMP PCR detection of the bacterial pathogen in potato plants and the psyllid vector. The advantage of the LAMP method is that it does not require a thermocycler for amplification or agarose gel electrophoresis for resolution. Positive LAMP results can be visualized directly as a precipitate. The LAMP strategy reported here reliably detected ‘Ca. Liberibacter solanacearum’ and the closely related species ‘Ca. Liberibacter asiaticus’, the causative agent of huanglongbing disease of citrus, in plant DNA extracts. Although not as sensitive as quantitative real-time PCR, LAMP detection was equivalent to conventional PCR in tests of ZC-infected potato plants from the field. Thus, the LAMP method shows strong promise as a reliable, rapid, and cost-effective method of detecting ‘Ca. Liberibacter’ pathogens in psyllids and field-grown potato plants and tubers.

scholarly journals A loop-mediated isothermal amplification procedure targeting the sodA gene for rapid and specific identification of Gallibacterium anatis

2018 ◽  
Vol 97 (4) ◽  
pp. 1141-1147 ◽  
Author(s):  
D. Stępień-Pyśniak ◽  
U. Kosikowska ◽  
T. Hauschild ◽  
A. Burzyński ◽  
J. Wilczyński ◽  
...  
Keyword(s):  
Isothermal Amplification ◽  
Specific Identification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Procedure

scholarly journals Development and Preliminary Evaluation of a Loop-Mediated Isothermal Amplification Procedure for Sensitive Detection of Cryptosporidium Oocysts in Fecal and Water Samples

2007 ◽  
Vol 73 (17) ◽  
pp. 5660-5662 ◽  
Author(s):  
Panagiotis Karanis ◽  
Oriel Thekisoe ◽  
Klytaimnistra Kiouptsi ◽  
Jerry Ongerth ◽  
Ikuo Igarashi ◽  
...  
Keyword(s):  
Diagnostic Tool ◽  
Water Samples ◽  
Epidemiologic Studies ◽  
Isothermal Amplification ◽  
Sensitive Detection ◽  
Preliminary Evaluation ◽  
Fecal Samples ◽  
Loop Mediated Isothermal Amplification ◽  
Cryptosporidium Oocysts ◽  
Amplification Procedure

ABSTRACT A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.

Loop-mediated isothermal amplification for rapid and convenient detection of Mycoplasma hyopneumoniae

2012 ◽  
Vol 29 (4) ◽  
pp. 607-616 ◽  
Author(s):  
Jiahe Li ◽  
F. Chris Minion ◽  
Andrew C. Petersen ◽  
Fei Jiang ◽  
Sheng Yang ◽  
...  
Keyword(s):  
Mycoplasma Hyopneumoniae ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification
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