scholarly journals A loop-mediated isothermal amplification procedure targeting the sodA gene for rapid and specific identification of Gallibacterium anatis

2018 ◽  
Vol 97 (4) ◽  
pp. 1141-1147 ◽  
Author(s):  
D. Stępień-Pyśniak ◽  
U. Kosikowska ◽  
T. Hauschild ◽  
A. Burzyński ◽  
J. Wilczyński ◽  
...  
Keyword(s):  
Isothermal Amplification ◽  
Specific Identification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Procedure

scholarly journals Development of a Loop-Mediated Isothermal Amplification Procedure as a Sensitive and Rapid Method for Detection of ‘Candidatus Liberibacter solanacearum’ in Potatoes and Psyllids

2012 ◽  
Vol 102 (9) ◽  
pp. 899-907 ◽  
Author(s):  
Aravind Ravindran ◽  
Julien Levy ◽  
Elizabeth Pierson ◽  
Dennis C. Gross
Keyword(s):  
Bacterial Pathogen ◽  
Cost Effective ◽  
Isothermal Amplification ◽  
Lamp Method ◽  
Candidatus Liberibacter Solanacearum ◽  
Loop Mediated Isothermal Amplification ◽  
Potato Plants ◽  
Cost Effective Method ◽  
Candidatus Liberibacter ◽  
Amplification Procedure

This study reports the development of a loop-mediated isothermal amplification procedure (LAMP) for polymerase chain reaction (PCR)-based detection of ‘Candidatus Liberibacter solanacearum’, the bacterial causal agent of potato zebra chip (ZC) disease. The 16S rDNA gene of ‘Ca. Liberibacter solanacearum’ was used to design a set of six primers for LAMP PCR detection of the bacterial pathogen in potato plants and the psyllid vector. The advantage of the LAMP method is that it does not require a thermocycler for amplification or agarose gel electrophoresis for resolution. Positive LAMP results can be visualized directly as a precipitate. The LAMP strategy reported here reliably detected ‘Ca. Liberibacter solanacearum’ and the closely related species ‘Ca. Liberibacter asiaticus’, the causative agent of huanglongbing disease of citrus, in plant DNA extracts. Although not as sensitive as quantitative real-time PCR, LAMP detection was equivalent to conventional PCR in tests of ZC-infected potato plants from the field. Thus, the LAMP method shows strong promise as a reliable, rapid, and cost-effective method of detecting ‘Ca. Liberibacter’ pathogens in psyllids and field-grown potato plants and tubers.

scholarly journals Real-Time Loop-Mediated Isothermal Amplification (RealAmp) for the Species-Specific Identification of Plasmodium vivax

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e54986 ◽  
Author(s):  
Jaymin C. Patel ◽  
Jenna Oberstaller ◽  
Maniphet Xayavong ◽  
Jothikumar Narayanan ◽  
Jeremy D. DeBarry ◽  
...  
Keyword(s):  
Plasmodium Vivax ◽  
Real Time ◽  
Isothermal Amplification ◽  
Specific Identification ◽  
Loop Mediated Isothermal Amplification ◽  
Species Specific ◽  
Time Loop

scholarly journals Molecular serotype-specific identification of Streptococcus pneumoniae using loop-mediated isothermal amplification

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Chika Takano ◽  
Yoko Kuramochi ◽  
Mitsuko Seki ◽  
Dong Wook Kim ◽  
Daisuke Omagari ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Detection Limits ◽  
Public Health Problem ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Lamp Method ◽  
Specific Identification ◽  
Loop Mediated Isothermal Amplification ◽  
Pcr Assays ◽  
Blood Specimens

AbstractIn children, the incidence of pneumococcal meningitis has decreased since the introduction of pneumococcal conjugate vaccine (PCV7 and PCV13). However, since the introduction of the vaccine, developed countries have seen the emergence of non-PCV13 serotypes. However, invasive pneumococcal disease (IPD) caused by PCV13-targeted serotypes still represents an important public health problem in resource-limited countries. To develop a rapid, simple, and cost-effective assay to detect serotypes of Streptococcus pneumoniae, we developed a novel loop-mediated isothermal amplification (LAMP) assay based on the sequences available for the 13 capsular types that are included in PCV13: 1, 3, 4, 5, 6 A, 6B, 7 F, 9 V, 14, 18 C, 19 A, 19 F, and 23 F. We evaluated test reactivity, specificity, sensitivity and performance, and compared the results between established LAMP and conventional PCR assays. To support its clinical use, the detection limits of the LAMP assay were evaluated using bacterial genomic DNA-spiked cerebrospinal fluid (CSF) and blood specimens. We confirmed the specificity of the LAMP assay using 41 serotypes of pneumococcal strains. The sensitivity of the LAMP assay was 10 to 100 copies per reaction, compared to 10 to 104 copies per reaction for PCR assays. The detection limits of the LAMP assay were comparable when using DNA-spiked CSF and blood specimens, as compared to using purified DNA as the template. In conclusion, a rapid and simple LAMP-based pneumococcal serotyping method has been developed. This is the first report of a LAMP method for a PCV13 serotype-specific identification assay, which could be a promising step to facilitate epidemiological studies of pneumococcal serotyping.

scholarly journals Development and Preliminary Evaluation of a Loop-Mediated Isothermal Amplification Procedure for Sensitive Detection of Cryptosporidium Oocysts in Fecal and Water Samples

2007 ◽  
Vol 73 (17) ◽  
pp. 5660-5662 ◽  
Author(s):  
Panagiotis Karanis ◽  
Oriel Thekisoe ◽  
Klytaimnistra Kiouptsi ◽  
Jerry Ongerth ◽  
Ikuo Igarashi ◽  
...  
Keyword(s):  
Diagnostic Tool ◽  
Water Samples ◽  
Epidemiologic Studies ◽  
Isothermal Amplification ◽  
Sensitive Detection ◽  
Preliminary Evaluation ◽  
Fecal Samples ◽  
Loop Mediated Isothermal Amplification ◽  
Cryptosporidium Oocysts ◽  
Amplification Procedure

ABSTRACT A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.

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