scholarly journals Differentiation of neonatal dorsal root ganglion-derived neural stem cells into oligodendrocytes after intrathecal transplantation into a cauda equina lesion model

2013 ◽  
Vol 12 (4) ◽  
pp. 6092-6102 ◽  
Author(s):  
Z.Y. Fu ◽  
J.G. Shi ◽  
N. Liu ◽  
L.S. Jia ◽  
W. Yuan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dorsal Root Ganglion ◽  
Neural Stem Cells ◽  
Dorsal Root ◽  
Cauda Equina ◽  
Intrathecal Transplantation ◽  
Cauda Equina Lesion

scholarly journals Transplantation of neural stem cells encapsulated in hydrogels improve functional recovery in a cauda equina lesion model

2020 ◽  
Author(s):  
Zhiyi Fu ◽  
Huidong Wang ◽  
Yujie Wu ◽  
Tong Zhu
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Functional Recovery ◽  
Cauda Equina ◽  
Three Dimensional ◽  
Stem Cell Differentiation ◽  
Blue Staining ◽  
Tail Flick ◽  
Pathological Changes ◽  
Cauda Equina Lesion

Abstract Background This study explored the therapeutic effects of transplantation of neural stem cells (NSCs) encapsulated in hydrogels in a cauda equina lesion model. Methods NSCs were isolated from neonatal dorsal root ganglion (DRG) and cultured in three-dimensional porous hydrogel scaffolds. Immunohistochemistry, transmission electron microscopy, Luxol fast blue staining, TUNEL assay were performed to detect the differentiation capability, ultrastructural and pathological changes, and apoptosis of NSCs. Furthermore, the functional recovery of sensorimotor reflexes was determined using the tail-flick test. Results NSCs derived from DRG were able to proliferate to form neurospheres and mainly differentiate into oligodendrocytes in the three-dimensional hydrogel culture system. After transplantation of NSCs encapsulated in hydrogels, NSCs differentiated into oligodendrocytes, neurons or astrocytes in vivo . Moreover, NSCs engrafted on the hydrogels decreased apoptosis and alleviated the ultrastructural and pathological changes of injured cauda equina. Behavioral analysis showed that transplanted hydrogel-encapsulated NSCs decreased the tail-flick latency and showed a neuroprotective role on injured cauda equina. Conclusions Our results indicate transplantation of hydrogel-encapsulated NSCs promotes stem cell differentiation into oligodendrocytes, neurons or astrocytes and contributes to the functional recovery of injured cauda equina, suggesting that NSCs encapsulated in hydrogels may be applied for the treatment of cauda equina injury.

scholarly journals Transplantation of neural stem cells encapsulated in hydrogels improve functional recovery in a cauda equina lesion model

2020 ◽  
Author(s):  
Zhiyi Fu ◽  
Huidong Wang ◽  
Yujie Wu ◽  
Tong Zhu
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Functional Recovery ◽  
Cauda Equina ◽  
Three Dimensional ◽  
Stem Cell Differentiation ◽  
Blue Staining ◽  
Tail Flick ◽  
Pathological Changes ◽  
Cauda Equina Lesion

Abstract Background This study explored the therapeutic effects of transplantation of neural stem cells (NSCs) encapsulated in hydrogels in a cauda equina lesion model.Methods NSCs were isolated from neonatal dorsal root ganglion (DRG) and cultured in three-dimensional porous hydrogel scaffolds. Immunohistochemistry, transmission electron microscopy, Luxol fast blue staining, TUNEL assay were performed to detect the differentiation capability, ultrastructural and pathological changes, and apoptosis of NSCs. Furthermore, the functional recovery of sensorimotor reflexes was determined using the tail-flick test.Results NSCs derived from DRG were able to proliferate to form neurospheres and mainly differentiate into oligodendrocytes in the three-dimensional hydrogel culture system. After transplantation of NSCs encapsulated in hydrogels, NSCs differentiated into oligodendrocytes, neurons or astrocytes in vivo . Moreover, NSCs engrafted on the hydrogels decreased apoptosis and alleviated the ultrastructural and pathological changes of injured cauda equina. Behavioral analysis showed that transplanted hydrogel-encapsulated NSCs decreased the tail-flick latency and showed a neuroprotective role on injured cauda equina.Conclusions Our results indicate transplantation of hydrogel-encapsulated NSCs promotes stem cell differentiation into oligodendrocytes, neurons or astrocytes and contributes to the functional recovery of injured cauda equina, suggesting that NSCs encapsulated in hydrogels may be applied for the treatment of cauda equina injury.

Dorsal root ganglion neurons induce transdifferentiation of mesenchymal stem cells along a Schwann cell lineage

2008 ◽  
Vol 445 (3) ◽  
pp. 246-251 ◽  
Author(s):  
Jinsong Yang ◽  
Qiang Lou ◽  
Renzheng Huang ◽  
Longxiang Shen ◽  
Zhengrong Chen
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dorsal Root Ganglion ◽  
Schwann Cell ◽  
Dorsal Root ◽  
Cell Lineage ◽  
Dorsal Root Ganglion Neurons ◽  
Ganglion Neurons

Adult mesenchymal stem cells support cisplatin-treated dorsal root ganglion survival

2008 ◽  
Vol 445 (1) ◽  
pp. 68-72 ◽  
Author(s):  
Arianna Scuteri ◽  
Elisabetta Donzelli ◽  
Maddalena Ravasi ◽  
Giovanni Tredici
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Adult Mesenchymal Stem Cells

scholarly journals Analgesia for Neuropathic Pain by Dorsal Root Ganglion Transplantation of Genetically Engineered Mesenchymal Stem Cells: Initial Results

2015 ◽  
Vol 11 ◽  
pp. s12990-015-0002 ◽  
Author(s):  
Hongwei Yu ◽  
Gregory Fischer ◽  
Allison D Ebert ◽  
Hsiang-En Wu ◽  
Xiaowen Bai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Neuropathic Pain ◽  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Genetically Engineered ◽  
Initial Results

Expression of Nogo-A in dorsal root ganglion in rats with cauda equina injury

2020 ◽  
Vol 527 (1) ◽  
pp. 131-137
Author(s):  
Xiaofei Sun ◽  
Qingjie Kong ◽  
Kaiqiang Sun ◽  
Le Huan ◽  
Ximing Xu ◽  
...  
Keyword(s):  
Dorsal Root Ganglion ◽  
Dorsal Root ◽  
Cauda Equina
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