scholarly journals Two splice variants of the bovine lactoferrin gene identified in Staphylococcus aureus isolated from mastitis in dairy cattle

2011 ◽  
Vol 10 (4) ◽  
pp. 3199-3203 ◽  
Author(s):  
J.M. Huang ◽  
Z.Y. Wang ◽  
Z.H. Ju ◽  
C.F. Wang ◽  
Q.L. Li ◽  
...  
2021 ◽  
Vol 15 (2) ◽  
pp. 140-145
Author(s):  
Amanda Pereira Lucas ◽  
Andriele Renata Barbosa de Farias ◽  
Elizabete Cristina da Silva ◽  
Kleber Régis Santoro ◽  
Marcelo Mendonça ◽  
...  

There are few reports in the literature about genetic determinants of resistance to β-lactams in Staphylococcus aureusisolated from dairy cattle located in the municipality of Garanhuns, state of Pernambuco, Brazil. Thus, this study aimed to investigate the production of β-lactamase and the presence of the blaZ and mecA genes in penicillin-resistant S. aureus isolated from cases of subclinical bovine mastitis in the city of Garanhuns. Forty-six strains of penicillin-resistant S. aureus were evalu-ated using the nitrocefin disc test and duplex PCR. The results revealed that 45 strains (97.8%) were positive for β-lactamase production and 44 (95.7%) carried the blaZ gene. Among the latter, 43 (97.7%) were β-lactamase producers and only one (2.3%) was not. The mecA gene was not detected in any of the isolates investigated. The results suggest that enzymatic inacti-vation is the main β-lactam resistance mechanism expressed by S. aureus in the herds analyzed.


2020 ◽  
Vol 9 (20) ◽  
Author(s):  
John D. Lippolis ◽  
Ellie J. Putz ◽  
Hao Ma ◽  
David P. Alt ◽  
Eduardo Casas ◽  
...  

Staphylococcus aureus can cause mastitis in dairy cattle. We report the genome sequence of a Staphylococcus aureus strain isolated from a dairy cow with a chronic case of mastitis. The infection with this strain of Staphylococcus aureus was not cleared from the animal with antibiotic treatment.


2020 ◽  
Vol 103 (3) ◽  
pp. 2685-2692
Author(s):  
A. Gazzola ◽  
A.M. Maisano ◽  
V. Bianchini ◽  
F. Vezzoli ◽  
A. Romanò ◽  
...  

2018 ◽  
Vol 59 (3) ◽  
pp. 357-363 ◽  
Author(s):  
Maria Belen Ambroggio ◽  
Melina Soledad Perrig ◽  
Cecilia Camussone ◽  
Nazarena Pujato ◽  
Alicia Bertón ◽  
...  

2012 ◽  
Vol 64 (11) ◽  
pp. 807-816 ◽  
Author(s):  
Xiuge Wang ◽  
Jinming Huang ◽  
Lihong Zhao ◽  
Changfa Wang ◽  
Zhihua Ju ◽  
...  

2001 ◽  
Vol 48 (5) ◽  
pp. 373-383 ◽  
Author(s):  
M. Hoedemaker ◽  
B. Korff ◽  
B. Edler ◽  
M. Emmert ◽  
E. Bleckmann

2015 ◽  
Vol 36 (4) ◽  
Author(s):  
Rajan Sharma ◽  
Dibyendu Chakraborty ◽  
Parul Gupta

Lactoferrin (Lf) was discovered in 1939 as “red protein from milk whey”. Bovine lactoferrin (bLf) gene is located on <italic>Bos taurus</italic> autosome, long arm of chromosome no.22 (BTA 22q24) in cattle. Its size varies from 23-35kbp among different species. The lactoferrin gene consists of 17 exons and 16 introns ranging from 82bp (exon-1) to 225bp (exon-17). The presence of multiple regulatory elements within lactoferrin promoter contributes differential gene expression and variable content of lactoferrin in milk. The concentration of lactoferrin in normal bovine milk is about 0.02-0.2 mg/ml. The primary function of Lf lies in its role in iron metabolism including iron transport, storage and chelation. Lf exhibits strong antimicrobial activity against a broad spectrum of bacteria (gram-positive & negative), fungi, yeasts, viruses and parasites. Lf exerts bacteriostatic and bactericidal activity. Its main contribution to antiviral defence consists in its binding to the cell membrane glycosaminoglycan, thus lactoferrin prevents viruses from entering cells and infection is stopped at an early age. More than 140 SNPs in this gene have been identiûed. Such a high variability in Lactoferrin gene implies that it may be used as candidate gene for screening animals also a marker of milk yield.


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