scholarly journals Antioxidant Activity Determination of Citronellal and Crude Extracts of <i>Cymbopogon citratus</i> by 3 Different Methods

2014 ◽  
Vol 05 (04) ◽  
pp. 395-400 ◽  
Author(s):  
Y. Lu ◽  
T. J. Khoo ◽  
C. Wiart
Keyword(s):  
Antioxidant Activity ◽  
Cymbopogon Citratus ◽  
Activity Determination ◽  
Crude Extracts

A Novel Standardized Oxygen Radical Absorbance Assay for Evaluating Antioxidant Natural Products

2013 ◽  
Vol 96 (6) ◽  
pp. 1365-1371 ◽  
Author(s):  
Odilia Nwadiche Osakwe ◽  
Andre Siegel
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Matrix Effect ◽  
Oxygen Radical ◽  
Complex Reaction ◽  
Dilution Factor ◽  
Multiple Components ◽  
Crude Extracts ◽  
Linear Correlations

Abstract Functional and quantitative evaluation of analytes in crude extracts or semipure mixtures has been considered challenging due to variation in generated results. Some of the drawbacks have been linked to the texture, form, and content of the samples. The crude extracts contain multiple components and, in oxygen radical absorbance capacity (ORAC) assays, have exhibited complex reaction kinetics due to interference or matrix effect. The conventional ORAC assay utilized a single dilution factor that brought about a wide CV difference (60–110%) in antioxidant activity for each extract compared to other dilution factors applied. Due to precision errors introduced from these extremely high CV% differences, determination of antioxidant capacity based on a single dilution may introduce discrepant results. A novel standardized ORAC (SORAC) assay for the determination of antioxidant capacity of dietary plant extracts is described in this study. Using the SORAC assay, positive linear correlations (R2 = 0.95–0.99) were obtained for the sample extracts tested when a multiple dilution strategy was applied. Thus, on the strength of our observations, SORAC has been proposed as an effective tool for assessing antioxidant analytes in a biological matrix. This technique could also be modified for use in other bioanalytical platforms concerned with the analyte matrix effect.

scholarly journals A Brief Overview on Antioxidant Activity Determination of Silver Nanoparticles

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3191 ◽  
Author(s):  
Zdenka Bedlovičová ◽  
Imrich Strapáč ◽  
Matej Baláž ◽  
Aneta Salayová
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Relevant Information ◽  
Review Article ◽  
Activity Determination ◽  
Methods Of Determination

Our objective in this review article is to find out relevant information about methods of determination of antioxidant activity of silver nanoparticles. There are many studies dealing with mentioned problem and herein we summarize the knowledge about methods evaluating the antioxidant activity of silver nanoparticles reported so far. Many authors declare better antioxidant activity of silver nanoparticles compared to the extract used for synthesis of them. In this review, we focused on methods of antioxidant activity determination in detail to find out novel and perspective techniques to solve the general problems associated with the determination of antioxidant activity of silver nanoparticles.

Phytochemical screening and antioxidant activity determination of some medicinally important plants of Balochistan

2018 ◽  
Vol 51 (2) ◽  
Author(s):  
Farida Behlil ◽  
Sami Ullah ◽  
Naqeebullah Khan ◽  
Ali Akbar ◽  
Rasool Bakhsh Tareen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phytochemical Screening ◽  
Activity Determination

KAJIAN KANDUNGAN FENOLAT DAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL TEMPE GEMBUS DARI BERBAGAI WAKTU INKUBASI

KOVALEN ◽  
2016 ◽  
Vol 2 (3) ◽  
Author(s):  
Mohammad Sidiq ◽  
Mappiratu Mappiratu ◽  
Nurhaeni Nurhaeni
Keyword(s):  
Antioxidant Activity ◽  
Incubation Time ◽  
Phenolic Antioxidant ◽  
Total Phenolic ◽  
Activity Time ◽  
Activity Determination ◽  
Use Of Time

The research about study of content phenolic and antioxidant activity extract ethanol tempeh gembus of a variety of incubation has been done. This study aims to know the time of incubation and time extraction that produced levels of the total phenolic and the best antioxidant activity. Determination of total phenolic using the method of folin-ciocalteu and antioxidant activity using the method DPPH. The achievement of goals be done through the application of the treatment of the time of incubation and time extraction of level of phenolic and antioxidant activity. The influence of the time of incubation is applied five levels of 0 hours; 12 hours; 24 hours; 36 hours and 48 hours. While the influence of time extraction is 1 hours, 1.5 hours, 2 hours, 2.5 hours, and 3 hours. From the result obtained by the incubation is at the time of incubation 36 hours which resulted in levels of total phenolic of 7.75 % and the value of IC50 0f 1098.88 µg/mL. The use of time extraction is 1,5 hours that produces levels of total phenolic of 5.26 % and the value of IC50 of 1198.95 µg/mL.Keyword: tempeh gembus, total phenolic, antioxidant activity, time of incubation, time extraction

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