scholarly journals Diagnosis of Bovine Tuberculosis by Comparative Intradermal Tuberculin Test, Interferon Gamma Assay and <i>esxB</i> (CFP-10) PCR in Blood and Lymph Node Aspirates

2019 ◽  
Vol 09 (05) ◽  
pp. 55-65
Author(s):  
Derhasar Brahma ◽  
Deepti Narang ◽  
Mudit Chandra ◽  
Gursimran Filia ◽  
Amarjit Singh ◽  
...  
Keyword(s):  
Lymph Node ◽  
Interferon Gamma ◽  
Bovine Tuberculosis ◽  
Tuberculin Test ◽  
Gamma Assay

Field comparison of the interferon-gamma assay and the intradermal tuberculin test for the diagnosis of bovine tuberculosis

1991 ◽  
Vol 68 (9) ◽  
pp. 286-290 ◽  
Author(s):  
PR WOOD ◽  
LA CORNER ◽  
JS ROTHEL ◽  
C. BALDOCK ◽  
SL JONES ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Bovine Tuberculosis ◽  
Tuberculin Test ◽  
Gamma Assay

Prevalence of Bovine Tuberculosis in Egyptian Cattle and the Standardization of the Interferon-gamma Assay as an Ancillary Test

2014 ◽  
Vol 63 (5) ◽  
pp. 497-507 ◽  
Author(s):  
G. S. Abdellrazeq ◽  
M. M. Elnaggar ◽  
H. S. Osman ◽  
W. C. Davis ◽  
M. Singh
Keyword(s):  
Interferon Gamma ◽  
Bovine Tuberculosis ◽  
Ancillary Test ◽  
Gamma Assay

scholarly journals High Prevalence and Increased Severity of Pathology of Bovine Tuberculosis in Holsteins Compared to Zebu Breeds under Field Cattle Husbandry in Central Ethiopia

2007 ◽  
Vol 14 (10) ◽  
pp. 1356-1361 ◽  
Author(s):  
Gobena Ameni ◽  
Abraham Aseffa ◽  
Howard Engers ◽  
Douglas Young ◽  
Stephen Gordon ◽  
...  
Keyword(s):  
Lymph Node ◽  
Lymph Nodes ◽  
Mesenteric Lymph Node ◽  
Bovine Tuberculosis ◽  
Control Program ◽  
Tuberculin Test ◽  
Skin Lesions ◽  
Mesenteric Lymph ◽  
Significant Difference ◽  
Cattle Husbandry

ABSTRACT A comparative study on the prevalence and pathology of bovine tuberculosis (TB) was conducted on 5,424 cattle (2,578 zebus, 1,921 crosses, and 925 Holsteins), which were kept on pasture in the central highlands of Ethiopia, using a comparative intradermal tuberculin test, postmortem examination, and bacteriology. The overall prevalence of bovine TB was 13.5%; prevalence was higher in Holsteins than either zebus (22.2% versus 11.6%, χ2 = 61.8; P < 0.001) or crosses (22.2% versus 11.9%, χ2 = 50.7; P < 0.001). Moreover, the severity of pathology in Holsteins (mean ± standard error of the mean [SEM], 6.84 ± 0.79) was significantly higher (P = 0.018) than the severity of pathology in zebus (5.21 ± 0.30). In addition, the risk of TB in Holsteins was more than twice (odds ratio [OR] = 2.32; 95% confidence interval [CI] = 1.89, 2.85) that in zebus. Animals between 5 and 9 years of age were at higher (OR = 2.37; 95% CI = 1.80, 3.12) risk of bovine TB than those 2 years of age or below. A significant difference (χ2 = 351; P < 0.001) in the occurrence of TB lesions in lymph nodes was recorded; the mesenteric lymph node (mean pathology score ± SEM, 1.95 ± 0.08) was most severely affected, followed by the retropharyngeal (0.80 ± 0.05) and caudal mediastinal (0.8 ± 0.06) lymph nodes. Fifty-six percent (n = 145) of the animals with gross TB lesions were culture positive; the lowest culture positivity was recorded in the skin lesions (27.3%) and the lesions of the mesenteric lymph node (31.5%). Both the skin test response and the postmortem findings suggested a higher susceptibility to bovine TB in Holsteins than zebus under identical field husbandry conditions (on pasture). In the light of increased numbers of Holstein cattle introduced into this area to raise milk production to satisfy the needs of Addis Ababa's growing population, these findings highlight the need for a control program in these herds.

Real-Time PCR Detection of Mycobacterium bovis in Blood and Lymph Node Aspirates of Bovines Positive in Tuberculosis Screening Tests

2020 ◽  
Author(s):  
D. Narang ◽  
G. S. Sidhu ◽  
G. Filia ◽  
A. Singh ◽  
S. T. Singh ◽  
...  
Keyword(s):  
Lymph Node ◽  
Real Time ◽  
Mycobacterium Bovis ◽  
Real Time Pcr ◽  
Bovine Tuberculosis ◽  
Tuberculin Test ◽  
Screening Tests ◽  
Low Molecular Weight ◽  
Tuberculosis Screening ◽  
Three Samples

Bovine tuberculosis is a chronic infectious disease affecting broad range of mammalian hosts. ESAT-6 is a low molecular weight immunodominant protein coded by esxA gene located on RD1 region of genome and is responsible for virulence of Mycobacterium bovis. Out of 200 animals screened for bovine Tuberculosis (bTB), 38 animals (19%) were found positive for Comparative intradermal tuberculin test (CITT) (32 cattle, 6 buffaloes) and 41 animals were tested positive by IFN-g assay (29 cattle, 12 buffaloes). DNA extraction of blood (n=200) and lymph node aspirates (n=48) was done. Among 200 blood samples targeted for esxA (ESAT-6) gene, three samples (1.5%) whose CT was between 23-34 were considered positive by real-time PCR. Out of 48 animals (lymph node aspirates) that were positive either by CITT or IFN-g, one sample (2.08%) whose CT was between 23-34 were considered positive by real-time PCR. Remaining samples whose CT values were equal to or greater than 35 were considered negative. The sensitivity of esxA was 8 pg/ìl by real time PCR.

scholarly journals Development and evaluation of an interferon gamma assay for the diagnosis of tuberculosis in red deer experimentally infected with Mycobacterium bovis

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
María Ángeles Risalde ◽  
Jobin Thomas ◽  
Iker Sevilla ◽  
Miriam Serrano ◽  
Jose Antonio Ortíz ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Interferon Gamma ◽  
Red Deer ◽  
Gamma Assay

scholarly journals Bovine tuberculosis in one cattle herd in Ibadan in Nigeria

2012 ◽  
Vol 49 (No. 11) ◽  
pp. 406-412 ◽  
Author(s):  
S. I B Cadmus ◽  
N. N Atsanda ◽  
S. O Oni ◽  
E. E U Akang
Keyword(s):  
Mycobacterium Bovis ◽  
Bovine Tuberculosis ◽  
Molecular Typing ◽  
Human Population ◽  
Tuberculin Test ◽  
Cattle Herd ◽  
Post Mortem ◽  
Risk Of Infection ◽  
Cultural Isolation ◽  
Male Animal

Bovine tuberculosis was investigated in one private herd with 171 cattle after five cases were suspected to be tuberculous following post mortem examination. Using the intradermal comparative cervical tuberculin test 18 (10.5%) animals (ages from 2 to 12 years) were positive: 11 animals of N&rsquo;dama breed and seven animals of White&nbsp;Fulani (i.e. Bunaji) breed; 17 female and one male animal. In all 11 randomly selected positive reactors, a spectrum of tuberculous lesions affecting the lungs, spleen, heart, liver, and the lymph nodes were observed. All the smear samples obtained were positive for acid-fast bacilli; cultural isolation confirmed the growth of mycobacteria on pyruvate-enriched Loewenstein-Jensen medium, which were identified by molecular typing to be Mycobacterium bovis. This study demonstrates widespread infection in this cattle herd and potential risk of infection for the human population with M. bovis.

Evaluation of the McLintock syringe as a cause of non-specific reactions in the intradermal tuberculin test used for the diagnosis of bovine tuberculosis

2019 ◽  
Vol 122 ◽  
pp. 175-178 ◽  
Author(s):  
A. Roy ◽  
A. Díez-Guerrier ◽  
J. Ortega ◽  
M.L. de la Cruz ◽  
J.L. Sáez ◽  
...  
Keyword(s):  
Bovine Tuberculosis ◽  
Tuberculin Test ◽  
Specific Reactions

scholarly journals Factors Affecting Herd Status for Bovine Tuberculosis in Dairy Cattle in Northern Thailand

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Tawatchai Singhla ◽  
Sukolrat Boonyayatra ◽  
Veerasak Punyapornwithaya ◽  
Kimberly L. VanderWaal ◽  
Julio Alvarez ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Bovine Tuberculosis ◽  
Case Control Study ◽  
Tuberculin Test ◽  
Dairy Farms ◽  
Case Control ◽  
Chiang Mai ◽  
Northern Thailand ◽  
Factors Affecting ◽  
Control Study

The objective of this case-control study was to identify farm-level risk factors associated with bovine tuberculosis (bTB) in dairy cows in northern Thailand. Spatial analysis was performed to identify geographical clustering of case-farms located in Chiang Mai and Chiang Rai provinces in northern Thailand. To identify management factors affecting bTB status, a matched case-control study was conducted with 20 case-farms and 38 control-farms. Case-farms were dairy farms with at least single intradermal tuberculin test- (SIT-) reactor(s) in the farms during 2011 to 2015. Control-farms were dairy farms with no SIT-reactors in the same period and located within 5 km from case-farms. Questionnaires were administered for data collection with questions based on epidemiological plausibility and characteristics of the local livestock industry. Data were analyzed using multiple logistic regressions. A significant geographic cluster was identified only in Chiang Mai province (p<0.05). The risk factor associated with presence of SIT-reactors in dairy herds located in this region was purchasing dairy cows from dealers (OR = 5.85, 95% CI = 1.66–20.58, and p=0.006). From this study, it was concluded that geographic clustering was identified for dairy farms with SIT-reactors in these provinces, and the cattle movements through cattle dealers increased the risks for SIT-reactor farm status.

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