scholarly journals Stress-Induced Changes in Testosterone Secretion in Male Rats: Role of Oxidative Stress and Modulation by Antioxidants

2014 ◽  
Vol 04 (02) ◽  
pp. 70-78 ◽  
Author(s):  
Mona Abdullah Al-Damegh
Keyword(s):  
Oxidative Stress ◽  
Male Rats ◽  
Testosterone Secretion ◽  
Induced Changes

p38 and ERK1/2 MAPKs mediate the interplay of TNF-α and IL-10 in regulating oxidative stress and cardiac myocyte apoptosis

2007 ◽  
Vol 293 (6) ◽  
pp. H3524-H3531 ◽  
Author(s):  
Sanjiv Dhingra ◽  
Anita K. Sharma ◽  
Dinender K. Singla ◽  
Pawan K. Singal
Keyword(s):  
Oxidative Stress ◽  
P38 Mapk ◽  
Cardiac Myocyte ◽  
Cell Injury ◽  
Male Rats ◽  
Tnf Α ◽  
Myocyte Apoptosis ◽  
Cardiac Myocyte Apoptosis ◽  
Induced Changes

It is known that TNF-α increases the production of ROS and decreases antioxidant enzymes, resulting in an increase in oxidative stress. IL-10 appears to modulate these effects. The present study investigated the role of p38 and ERK1/2 MAPKs in mediating the interplay of TNF-α and IL-10 in regulating oxidative stress and cardiac myocyte apoptosis in Sprague-Dawley male rats. Isolated adult cardiac myocytes were exposed to TNF-α (10 ng/ml), IL-10 (10 ng/ml), and IL-10 + TNF-α ( ratio 1) for 4 h. H2O2(100 μM) as a positive control and the antioxidant Trolox (20 μmol/l) were used to confirm the involvement of oxidative stress. H2O2treatment increased oxidative stress and apoptosis; TNF-α mimicked these effects. Exposure to TNF-α significantly increased ROS production, caused cell injury, and increased the number of apoptotic cells and Bax-to-Bcl-xl ratio. This change was associated with an increase in the phospho-p38 MAPK-to-total p38 MAPK ratio and a decrease in the phospho-ERK1/2-to-total ERK1/2 ratio. IL-10 treatment by itself had no effect on these parameters, but it prevented the above-listed changes caused by TNF-α. The antioxidant Trolox modulated TNF-α-induced changes in Bax/Bcl-xl, cell injury, and MAPKs. Preexposure of cells to the p38 MAPK inhibitor SB-203580 prevented TNF-α-induced changes. Inhibition of the ERK pathway with PD-98059 attenuated the protective role of IL-10 against TNF-α-induced apoptosis. This study provides evidence in support of the essential role of p38 and ERK1/2 MAPKs in the interactive role of TNF-α and IL-10 in cardiac myocyte apoptosis.

scholarly journals The protective role of CsNPs and CurNPs against DNA damage, oxidative stress, and histopathological and immunohistochemical alterations induced by hydroxyapatite nanoparticles in male rat kidney

2019 ◽  
Vol 8 (5) ◽  
pp. 741-753 ◽  
Author(s):  
Israa F. Mosa ◽  
Mokhtar I. Yousef ◽  
Maher Kamel ◽  
Osama F. Mosa ◽  
Yasser Helmy
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Biological Effects ◽  
Male Rats ◽  
Nuclear Antigen ◽  
Male Rat ◽  
Control Group ◽  
Renal Tubules ◽  
Hydroxyapatite Nanoparticles

Abstract Hydroxyapatite nanoparticles (HAP-NPs) are an inorganic component of natural bone and are mainly used in the tissue engineering field due to their bioactivity, osteoconductivity, biocompatibility, non-inflammatory, and non-toxicity properties. However, the current toxicity data for HAP-NPs regarding human health are limited, and only a few results from basic studies have been published. Therefore, the present study was designed to investigate the beneficial role of chitosan nanoparticles (CsNPs) and curcumin nanoparticles (CurNPs) in alleviating nephrotoxicity induced by HAP-NPs in male rats. The results showed that HAP-NPs caused a reduction in antioxidant enzymes and induced lipid peroxidation, nitric oxide production and DNA oxidation. Moreover, HAP-NP administration was associated with intense histologic changes in kidney architecture and immunoreactivity to proliferating cell nuclear antigen (PCNA). However, the presence of CsNPs and/or CurNPs along with HAP-NPs reduced the levels of oxidative stress through improving the activities of antioxidant enzymes. Also, the rats administered the nanoparticles showed a moderate improvement in glomerular damage which matched that of the control group and showed mild positive reactions to PCNA–ir in glomeruli and renal tubules in the cortical and medullary portions. These novel insights confirm that the presence of chitosan and curcumin in nanoforms has powerful biological effects with enhanced bioactivity and bioavailability phenomena compared to their microphase counterparts. Also, they were able to ameliorate the nephrotoxicity induced by HAP-NPs.

Role of Oxidative Stress in Ischemia–Reperfusion-Induced Changes in Na+,K+-ATPase Isoform Expression in Rat Heart

2004 ◽  
Vol 6 (5) ◽  
pp. 914-923 ◽  
Author(s):  
Petr Ostadal ◽  
Adel B. Elmoselhi ◽  
Irena Zdobnicka ◽  
Anton Lukas ◽  
Vijayan Elimban ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Heart ◽  
Ischemia Reperfusion ◽  
Isoform Expression ◽  
Induced Changes

Role of Oxidative Stress in Ischemia–Reperfusion-Induced Changes in Na+,K+-ATPase Isoform Expression in Rat Heart

2004 ◽  
Vol 6 (5) ◽  
pp. 914-923 ◽  
Author(s):  
Petr Ostadal ◽  
Adel B. Elmoselhi ◽  
Irena Zdobnicka ◽  
Anton Lukas ◽  
Vijayan Elimban ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Heart ◽  
Ischemia Reperfusion ◽  
Isoform Expression ◽  
Induced Changes

Protective Role of Quercetin on Polychlorinated Biphenyls (Aroclor-1254) Induced Oxidative Stress and Apoptosis in Liver of Adult Male Rats

2012 ◽  
Vol 26 (12) ◽  
pp. 522-532 ◽  
Author(s):  
Suganya Sekaran ◽  
Selvakumar Kandaswamy ◽  
Krishnamoorthy Gunasekaran ◽  
Elumalai Perumal ◽  
Fariya Yasmine Afsar Basha ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Polychlorinated Biphenyls ◽  
Adult Male ◽  
Protective Role ◽  
Male Rats ◽  
Aroclor 1254
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