Protective Role of Quercetin on Polychlorinated Biphenyls (Aroclor-1254) Induced Oxidative Stress and Apoptosis in Liver of Adult Male Rats

2012 ◽  
Vol 26 (12) ◽  
pp. 522-532 ◽  
Author(s):  
Suganya Sekaran ◽  
Selvakumar Kandaswamy ◽  
Krishnamoorthy Gunasekaran ◽  
Elumalai Perumal ◽  
Fariya Yasmine Afsar Basha ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Polychlorinated Biphenyls ◽  
Adult Male ◽  
Protective Role ◽  
Male Rats ◽  
Aroclor 1254

scholarly journals The Effect of Induced Diabetes Mellitus on the Cerebellar Cortex of Adult Male Rat and the Possible Protective Role of Oxymatrine: A Histological, Immunohistochemical and Biochemical Study

2021 ◽  
Author(s):  
Amany Mohamed Shalaby ◽  
Adel Mohamed Aboregela ◽  
Mohamed Ali Alabiad ◽  
Mona Tayssir Sadek
Keyword(s):  
Diabetes Mellitus ◽  
Purkinje Cells ◽  
Cerebellar Cortex ◽  
Adult Male ◽  
Protective Role ◽  
Diabetic Rats ◽  
Male Rats ◽  
Group I ◽  
Group Ii

Abstract Diabetes mellitus (DM) represents a widespread metabolic disease with a well-known neurotoxicity in both central and peripheral nervous systems. Oxymatrine is a traditional Chinese herbal medicine that has various pharmacological activities including; anti-oxidant, anti-apoptotic and anti-inflammatory potentials. The present work aimed to study the impact of diabetes mellitus on the cerebellar cortex of adult male albino rat and to evaluate the potential protective role of oxymatrine using different histological methods. Fifty-five adult male rats were randomly divided into three groups: group I served as control, group II was given oxymatrine (80 mg/kg/day) orally for 8 weeks and group III was given a single dose of streptozotocin (50mg/kg) intaperitoneally to induce diabetes. Then diabetic rats were subdivided into two subgroups: subgroup IIIa that received no additional treatment and subgroup IIIb that received oxymatrine similar to group II. The diabetic group revealed numerous changes in the Purkinje cell layer in the form of multilayer arrangement of Purkinje cells, shrunken cells with deeply stained nuclei as well as focal loss of the Purkinje cells. A significant increment in GFAP and synaptophysin expression was reported. Transmission electron microscopy showed irregularity and splitting of myelin sheaths in the molecular layer, dark shrunken Purkinje cells with ill-defined nuclei, dilated Golgi saccules and dense granule cells with irregular nuclear outlines in the granular layer. In contrast, these changes were less evident in diabetic rats that received oxymatrine. In conclusion, Oxymatrine could protect the cerebellar cortex against changes induced by DM.

Role of Quercetin on PCBs (Aroclor-1254) Induced Impairment of Dopaminergic Receptor mRNA Expression in Cerebral Cortex of Adult Male Rats

2011 ◽  
Vol 36 (8) ◽  
pp. 1344-1352 ◽  
Author(s):  
Rasiah Pratheepa Kumari ◽  
Kandaswamy Selvakumar ◽  
Senthamilselvan Bavithra ◽  
Rafiq Zumaana ◽  
Gunasekaran Krishnamoorthy ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Mrna Expression ◽  
Adult Male ◽  
Male Rats ◽  
Aroclor 1254 ◽  
Dopaminergic Receptor ◽  
Receptor Mrna

Protective role of Lycopene against Aroclor 1254-induced changes on GLUT4 in the skeletal muscles of adult male rat

2012 ◽  
Vol 36 (3) ◽  
pp. 320-328 ◽  
Author(s):  
Anne Augustine Williams ◽  
Jayaraman Selvaraj ◽  
Chinnapaiyan Srinivasan ◽  
Sampath Sathish ◽  
Parsanathan Rajesh ◽  
...  
Keyword(s):  
Adult Male ◽  
Skeletal Muscles ◽  
Protective Role ◽  
Male Rat ◽  
Aroclor 1254 ◽  
Adult Male Rat ◽  
Induced Changes

scholarly journals Effect of Flavonoids Extracted from Black Cumin (Nigella sativa) and Vitamin E in Ameliorating Hepatic Damage Induced by Sodium Nitrate in adult male rats

2012 ◽  
Vol 36 (0E) ◽  
pp. 172-181
Author(s):  
Baraa N. AL-Okaily
Keyword(s):  
Vitamin E ◽  
Adult Male ◽  
Sodium Nitrate ◽  
Nigella Sativa ◽  
Hemoglobin Concentration ◽  
Protective Role ◽  
Hepatic Damage ◽  
Male Rats ◽  
Bilirubin Concentration

This study was designed to investigate the effect of sodium nitrate as oxidant agent on hepatic function of adult male rats, as well as the possible protective role of vitamin E and flavonoid extracted from Nigella Sativa seeds against the deleterious effects of sodium nitrate. Forty adult male rats were randomly divided in to 4 equal groups and treated daily for 84 days as follows: Animals in the first group were received normal saline, serving as control (group C), rats of the second group (T1) were intubated orally sodium nitrate 30mg/kg. B.W.; animals in T2 group were intubated orally vit. E 40mg/Kg B.W. in addition to sodium nitrate, while rats in the fourth group (T3) were intubated orally 50mg/kg B.W of flavonoids was extracted from Nigella Sativa seeds with sodium nitrate. Blood samples were collected at 0, 21, 42, 63 and 84 days of experiment to study the following parameters: Serum alanine aminotransferase (ALT) and alkaline phosphtase activity (ALP), serum billirubin, as well as hemoglobin concentration. The result revealed that oral intubation of 30mg/kg. B.W of sodium nitrate (T1 group) for 84 days caused hepatic damage manifested by significant increase (p<0.05) in serum ALT and ALP activities, bilirubin concentration and depression in hemoglobin concentration. On other hand, the protective role of vitamin E and flavonoids extracted from Nigella Sativa was clarified in groupsT2 and T3, including correction of hepatic damage manifested by significant (p<0.05) depression in ALT and ALP activities and bilirubin concentration as well as significant (p<0.05) elevation in hemoglobin concentration. In conclusion, the results of this study confirm the protective role of vitamin E and flavonoids of Nigella sativa seed against hepatic dysfunction caused by sodium nitrate manifested by structural and functional changes.

scholarly journals Ameliorating Iron Overload in Intestinal Tissue of Adult Male Rats: Quercetin vs Deferoxamine

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Arwa A. El-Sheikh ◽  
Shimaa Hamed Ameen ◽  
Samaa Salah AbdEl-Fatah
Keyword(s):  
Oxidative Stress ◽  
Iron Overload ◽  
Adult Male ◽  
Caspase 3 ◽  
Iron Chelation ◽  
Male Rats ◽  
Albino Rats ◽  
Tissue Iron ◽  
Small Intestinal

Objective. The aim of our study is to compare the role of the new natural alternative (Quercetin) with the current iron-chelation therapy (Deferoxamine (DFO)) in the effect of iron overload on small intestinal tissues and to investigate the possible underlying molecular mechanisms of such toxicity. Methods. Forty-two adult male albino rats were divided into six groups: control groups, DFO, Quercetin, iron overload, iron overload+DFO, and iron overload+Quercetin groups. Animals received daily intraperitoneal injection of Deferoxamine (125 mg /kg), Quercetin (10 mg/kg), and ferric dextran (200 mg/kg) for 2 weeks. Results. Iron overloaded group showed significant increase in serum iron, total iron binding capacity (TIBC), transferrin saturation percentage (TS %) hepcidin (HEPC), serum ferritin, nontransferrin bound iron (NTBI), and small intestinal tissues iron levels. Iron overload significantly increased the serum oxidative stress indicator (MDA) and reduced serum total antioxidant capacity (TAC). On the other hand, iron overload increased IL6 and reduced IL10 in small intestinal tissues reflecting inflammatory condition and increased caspase 3 reactivity indicating apoptosis and increased iNOs expressing cell indicting oxidative stress especially in ileum. In addition, it induced small intestinal tissues pathological alterations. The treatment with Quercetin showed nonsignificant differences as compared to treatment with DFO that chelated the serum and tissue iron and improved the oxidative stress and reduced tissue IL6 and increased IL10 and decreased caspase 3 and iNOs expressing cells in small intestinal tissues. Moreover, it ameliorated the iron overload induced pathological alterations. Conclusion. Our study showed the potential role of Quercetin as iron chelator like DFO in case of iron overload induced small intestinal toxicity in adult rats because of its serum and tissue iron chelation, improvement of serum, and small intestinal oxidative stress, ameliorating iron induced intestinal inflammation, apoptosis, and histopathological alterations.

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