Nutrient Composition, Antioxidant Components and Ascorbic Acid Content Response of Pepper Fruit (Capsicum annuum L.) Cultivars Grown under Salt Stress

Investigations on the ascorbic acid content during subsequent stages of ripeness pepper fruits submitted to the influence of El and irradiation by 60Co. Considerable variation has been found in the ascorbic acid content in the pepper fruit, depending on the ripeness stage, the El concentration and the dose of 60Co. The highest content of this compound has been found at the intermediate ripeness stage, the lowest – at the technical ripeness stage. El caused vit. C increase at the concentration of 0.03% and 0.125%, and 60Co – at the 5000 Rtg dose.

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Obtaining Salt Stress-Tolerant Eggplant Somaclonal Variants from In Vitro Selection

Plants ◽

10.3390/plants10112539 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2539

Author(s):

Sami Hannachi ◽

Stefaan Werbrouck ◽

Insaf Bahrini ◽

Abdelmuhsin Abdelgadir ◽

Hira Affan Siddiqui ◽

...

Keyword(s):

Ascorbic Acid ◽

Salt Stress ◽

Water Content ◽

Acid Content ◽

In Vitro Selection ◽

Callus Tissue ◽

Salt Content ◽

Ascorbic Acid Content ◽

Control Line

An efficient regeneration protocol was applied to regenerate shoots on salt stress-tolerant calli lines of aubergine (Solanum melongena). These NaCl-tolerant cell lines were obtained by two different methods. On the one hand, the developed callus tissue was transferred to a medium with a continuous salt content of 40, 80, 120, or 160 mM NaCl. On the other hand, the callus tissue was subjected to a stepwise increasing salinity to 160 mM NaCl every 30 days. With the second method, calli which could be selected were characterized by compact growth, a greenish color, and absence of necrotic zones. When grown on salt-free medium again, NaCl-tolerant calli showed a decline in relative growth rate and water content in comparison to the control line. This was more obvious in the 120 mM NaCl-tolerant callus. Lipid peroxidase activity increased in 40 and 80 mM NaCl-tolerant calli; yet did not increase further in 120 mM-tolerant callus. An increase in ascorbic acid content was observed in 80 and 120 mM NaCl-tolerant calli compared to the 40 mM NaCl-tolerant lines, in which ascorbic acid content was twice that of the control. All NaCl-tolerant lines showed significantly higher superoxide dismutase (SOD) (208–305–370 µmol min−1 mg−1 FW) and catalase (CAT) (136–211–238 µmol min−1 mg−1 FW) activities compared to control plants (231 and 126 µmol min−1 mg−1 FW). Plants were regenerated on the calli lines that could tolerate up to 120 mM NaCl. From the 32 plants tested in vitro, ten plants with a higher number of leaves and root length could be selected for further evaluation in the field. Their high salt tolerance was evident by their more elevated fresh and dry weight, their more increased relative water content, and a higher number and weight of fruits compared to the wild-type parental control. The presented work shows that somaclonal variation can be efficiently used to develop salt-tolerant mutants.

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Effect of NaCl salt stress on the proline and ascorbic acid content of isabgol(Plantago ovataForsk.) genotypes

Research on Crops ◽

10.5958/2348-7542.2015.00109.6 ◽

2015 ◽

Vol 16 (4) ◽

pp. 768

Author(s):

Suraj Kala ◽

U. K. Varshney

Keyword(s):

Ascorbic Acid ◽

Salt Stress ◽

Acid Content ◽

Ascorbic Acid Content

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Provitamin A and Ascorbic Acid Content of Fresh Pepper Cultivars (Capsicum annuum) and Processed Jalapeños

Journal of Food Science ◽

10.1111/j.1365-2621.1994.tb06967.x ◽

1994 ◽

Vol 59 (2) ◽

pp. 362-365 ◽

Cited By ~ 125

Author(s):

L. R. HOWARD ◽

R. T. SMITH ◽

A. B. WAGNER ◽

B. VILLALON ◽

E. E. BURNS

Keyword(s):

Ascorbic Acid ◽

Capsicum Annuum ◽

Acid Content ◽

Ascorbic Acid Content ◽

Provitamin A

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508 Ascorbic Acid Content in Tomato and Bell Pepper Fruit during Development, Maturation, and Senescence on the Plant and Relation with Ascorbate Oxidase and Polyamines

HortScience ◽

10.21273/hortsci.34.3.533b ◽

1999 ◽

Vol 34 (3) ◽

pp. 533B-533

Author(s):

E.M. Yahia ◽

M. Contreras-Padilla ◽

G. Gonzalez-Aguilar

Keyword(s):

Ascorbic Acid ◽

Acid Content ◽

Fruit Set ◽

Tomato Fruit ◽

Ascorbic Acid Content ◽

Bell Pepper ◽

Ascorbate Oxidase ◽

Pepper Fruit ◽

Hydroponic Conditions

Plants were grown in a greenhouse in controlled hydroponic conditions, and fruit were harvested at different intervals from 18 to 94 days from fruit set (DFFS). AsA was higher in pepper than in tomato fruit. In pepper fruit, it increased very fast and reached a maximum at 50 DFFS and then decreased suddenly and reached a minimum about 60 DFFS. AsA in tomato fruit increased slowly and reached a maximum after about 75 DFFS and then declined slowly. The decrease in AsA is correlated with the initiation of ripening and with an increase in the activity of AAO. There was more putrescine in pepper than in tomato fruit. There were no major changes in spermine and spermidine in pepper and spermidine in tomato fruit. However, putrescine in pepper fruit and spermine in tomato fruit increased very early until about 30 to 38 DFFS and then decreased in a manner similar to the decrease in AsA. Putrescine in tomato fruit increased consistently and did not decrease even during the senescence of the fruit. We conclude that AsA decreases in tomato and pepper fruit after 75 and 50 DFFS, respectively, and this decrease is related to an increase in AAO and a decrease in putrescine in pepper and spermine in tomato fruit.

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A simple and rapid electrochemical determination of pungency: Application to aqueous and ethanolic extracts of Capsicum annuum cubana red

Journal of Chemical Research ◽

10.1177/1747519820930965 ◽

2020 ◽

pp. 174751982093096

Author(s):

María T Moreno ◽

Rafael Estévez Brito ◽

Marina Córdoba ◽

José Miguel Rodríguez Mellado

Keyword(s):

Ascorbic Acid ◽

Capsicum Annuum ◽

Acid Content ◽

Limit Of Detection ◽

Phosphate Buffer Solution ◽

Ascorbic Acid Content ◽

Differential Pulse ◽

Linear Range ◽

Dynamic Linear Range

Chili peppers are rich in ascorbic acid and capsaicin. In this article is proposed an easy, fast, sensitive, and inexpensive method for determining the pungency and content of ascorbic acid in chili pepper extracts. The voltammetric (cyclic and differential pulse) behavior of capsaicin on a glassy carbon electrode has been evaluated at different pH values. A calibration curve has been obtained for the peak current, IP, of capsaicin as a function of the capsaicin concentration, C, in differential pulse voltammetry in phosphate buffer solution at pH 7.0: IP(µA) = 0.0147 (±5.346·10−3) + 0.0507 (±8.984·10−4) C(µM), with limit of detection, LOD = 0.198µM, limit of quantification, LOQ = 0.660µM, and dynamic linear range from 0.660 to 20.9 µM. A variant of the standard addition method has been used for simultaneous determination of the pungency and ascorbic acid content of extracts of Capsicum annuum cubana red. In this case, the calibration for ascorbic acid was I(µA) = 0.467 (±0.012) + 2.039·10−3 (±4.601·10−5) VAA(L)), with LOD = 17.56 µL, LOQ = 58.55 µL, and dynamic linear range from 58.6 to 500 µL, being VAA the volume of 10-mM ascorbic acid added to 50 mL of solution. The ascorbic acid content was compared to that of a sweet pepper. The method is cheap, simple, and fast (30 min vs c.a. 2 h compared to the spectrophotometric method), its sensitivity being comparable to other more expensive and/or more laborious methods.

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Physiological and Biochemical Responses of Ungrafted and Grafted Bell Pepper Plants (Capsicum annuum L. var. grossum (L.) Sendtn.) Grown under Moderate Salt Stress

Plants ◽

10.3390/plants10020314 ◽

2021 ◽

Vol 10 (2) ◽

pp. 314

Author(s):

Nina Kacjan Maršić ◽

Petra Štolfa ◽

Dominik Vodnik ◽

Katarina Košmelj ◽

Maja Mikulič-Petkovšek ◽

...

Keyword(s):

Salt Stress ◽

Capsicum Annuum ◽

Water Status ◽

Bell Pepper ◽

Quality Trait ◽

Physiological Level ◽

Mediation Effects ◽

Capsicum Annuum L ◽

Pepper Fruit ◽

Pepper Plants

The response of grafted bell pepper plants (Capsicum annuum L. var. grossum (L.) Sendtn.) to salt stress was investigated by analyzing the photosynthetic traits and mineral content of the plants and the metabolic composition of the fruit. The bell pepper variety “Vedrana” was grafted onto the salt-tolerant rootstock “Rocal F1” and grown at two salinities (20 mM and 40 mM NaCl) and control (0 mM NaCl) during the spring–summer period. On a physiological level, similar stomatal restriction of photosynthesis in grafted and ungrafted plants indicated that grafting did not alleviate water balance disturbances under increased salt exposure. Measurements of midday water potential did not show improved water status of grafted plants. The similar metabolic changes in grafted and ungrafted plants were also reflected in similarly reduced fruit yields. Thus, this grafting did not reduce the risk of ionic and osmotic imbalance in pepper plants grown under moderate salt treatment. Changes in the biochemical profiles of the pepper fruit were seen for both added-salt treatments. The fruit phenolic compounds were affected by rootstock mediation, although only for the July harvest, where total phenolics content increased with 40 mM NaCl treatment. Fruit ascorbic acid content increased with the duration of salt stress, without the mediation of the rootstock. The high salt dependence of this quality trait in pepper fruit appears to lead to more limited rootstock mediation effects.

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Selection of F3 populations of Capsicum annuum for greenhouse production

Australian Journal of Crop Science ◽

10.21475/ajcs.21.15.03.p3046 ◽

2021 ◽

pp. 438-444

Author(s):

Laura Raquel Luna García ◽

Valentín Robledo Torres ◽

Francisca Ramírez Godina ◽

Rosalinda Mendoza Villarreal ◽

Miguel Ángel Pérez Rodríguez

Keyword(s):

Ascorbic Acid ◽

Capsicum Annuum ◽

Acid Content ◽

Genetic Recombination ◽

Block Design ◽

Research Work ◽

Ascorbic Acid Content ◽

Fruit Weight ◽

High Yield ◽

Greenhouse Production

Capsicum annuum is one of the most important plant species in the world. México has the greatest diversity for this plant. However, its production is limited due to the scarcity of improved varieties for greenhouse production. Therefore, the development of high-yield varieties would be possible through the genetic recombination of native varieties (Creole populations) and superior cultivars. Therefore, the purpose of this research work was to assess and select outstanding F3 populations for greenhouse production. The work was carried out in a greenhouse at Saltillo, Coahuila Mexico in 2018, involving 8 parents, in which 3 varieties were used as female (pollen-receptor plants), 5 varieties as males (pollen-donor plants) and 9 F3 populations derived by selfing from 9 F2 populations. The parents and F3 populations composed 17 treatments that were established in a greenhouse under a randomized block design with three replications. The variables were total fruit yield, average fruit weight, total number of fruits per plant, ascorbic acid content, total capsicinoids, days to flowering, days to harvest, final plant height, and basal stem diameter. Significant differences were found in all variables of F3 populations. The highest RTF (total fruit weight) belonged to P1,4 and P1,6, with1647.0 and 1652.0 g/plant, respectively. In terms of CAA (ascorbic acid content), population P2,4, was significantly superior to the rest of the genotypes and exceeded the best parent by 19.8%. We concluded that populations P1,4, P1,6 and P2,4 may be uased to develop cultivars with high yield and high quality for greenhouse production

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