scholarly journals <i>Blastomyces dermatitidis</i> Antibody Responses in Serial Serum Specimens from Dogs with Blastomycosis: Comparison of Different Yeast Lysate Antigens

2014 ◽  
Vol 02 (02) ◽  
pp. 67-73
Author(s):  
Mara Shepherd ◽  
Misty Lutes ◽  
Gene Scalarone
Keyword(s):  
Antibody Responses ◽  
Blastomyces Dermatitidis ◽  
Yeast Lysate ◽  
Serial Serum ◽  
Lysate Antigens

Detection of antibody responses and delayed dermal hypersensitivity with Blastomyces dermatitidis yeast and mycelial lysate antigens

1991 ◽  
Vol 114 (3) ◽  
pp. 137-144 ◽  
Author(s):  
B. W. Seawell ◽  
J. Y. Wheeler ◽  
K. Yearsley ◽  
K. L. Alexander ◽  
A. M. Legendre ◽  
...  
Keyword(s):  
Antibody Responses ◽  
Blastomyces Dermatitidis ◽  
Lysate Antigens

scholarly journals Blastomyces dermatitidis: Stability studies on different yeast lysate antigens

2013 ◽  
Vol 03 (03) ◽  
pp. 98-102 ◽  
Author(s):  
Tiffany R. Allison ◽  
Joshua C. Wright ◽  
Gene M. Scalarone
Keyword(s):  
Blastomyces Dermatitidis ◽  
Stability Studies ◽  
Yeast Lysate ◽  
Lysate Antigens

scholarly journals Blastomyces dermatitidisYeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Alex R. Boyd ◽  
Jamie L. VanDyke ◽  
Gene M. Scalarone
Keyword(s):  
Comparative Study ◽  
Fungal Infection ◽  
Antibody Detection ◽  
Blastomyces Dermatitidis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diagnostic Challenge ◽  
Systemic Fungal Infection ◽  
Yeast Lysate ◽  
The Individual ◽  
Lysate Antigens

The systemic fungal infection, blastomycosis, which infects both humans and animals has presented a diagnostic challenge for clinicians for many years. The aim of this study was to evaluate the diagnostic sensitivity ofBlastomyces dermatitidisyeast lysate antigens with respect to antibody detection in dogs with blastomycosis. Lysate antigens were prepared fromB. dermatitidisisolates T-58 and T-66 (dogs, Tennessee) and WI-R and WI-J (dogs, Wisconsin). Based on results obtained from a preliminary comparative study, five combinations of these isolates and one individual isolate were tested against 92 serum specimens from dogs with culture-proven or histologically-confirmed blastomycosis, using the indirect enzyme-linked immunosorbent assay (ELISA). Mean absorbance values obtained from the sera ranged from 0.905 with the individual T-58 antigen to 1.760 using an antigen combination (T-58 + T-66 + WI-R). All of the 6 antigenic preparations were able to detect antibody in the serum specimens, but the antigen combinations detected antibody to a higher degree than the individual antigen. This study provides evidence that combinations of the yeast lysate reagents seem to be more efficacious for antibody detection in dog sera, but our laboratory is continuing to evaluate antigen lysate combinations for detection of antibodies in blastomycosis.

scholarly journals Comparison of Antibody Detection with Yeast Lysate Antigens Prepared from Blastomyces dermatitidis Dog Isolates from Wisconsin and Tennessee

2013 ◽  
Vol 03 (01) ◽  
pp. 67-72 ◽  
Author(s):  
Jessica J. Roberts ◽  
Michael V. Madrid ◽  
Lindsy Dickerson ◽  
Bradi Hutchison ◽  
Gene M. Scalarone
Keyword(s):  
Antibody Detection ◽  
Blastomyces Dermatitidis ◽  
Yeast Lysate ◽  
Lysate Antigens

scholarly journals Blastomyces dermatitidis: Antibody Detection in Sera from Dogs with Blastomycosis with Yeast Lysate Antigens Produced from Human and Dog Isolates

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Katie Mondada ◽  
Jessie Fullmer ◽  
Eric Hungerford ◽  
Katrina Novack ◽  
Kristen Vickers ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Systemic Disease ◽  
Human Isolate ◽  
Antibody Detection ◽  
Blastomyces Dermatitidis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Yeast Lysate ◽  
Lysate Antigens

Dogs are common hosts to the fungal organismBlastomyces dermatitidis, which causes the systemic disease blastomycosis. The goal of our study was to compare the reactivity of twoB. dermatitidisyeast lysate antigens prepared from dog isolates (ERC-2, Wisconsin; T-58, Tennessee) and two lysate antigens prepared from human isolates (B5931 and B5896, Minnesota) against 48 serum specimens from dogs with confirmed blastomycosis using the indirect enzyme-linked immunosorbent assay (ELISA). Secondarily, we used three different ELISA substrates (Ultra TMB: A, SureBlue: B, and SureBlue Reserve: C) to compare the effectiveness of each substrate. Mean absorbance values ranged from 0.446 (B) to 0.651 (C) for the B5931 antigen and from 0.393 (B) to 0.540 (C) for the ERC-2 antigen in Trial 1. In Trial 2, the absorbance values ranged from 0.628 (B) to 0.909 (A) for the B5896 antigen and from 0.828 (B) to 1.375 (C) for the T-58 antigen. In Trial 1, the lysate antigen prepared from the human isolate B5931 exhibited the highest absorbance value and in Trial 2 the lysate prepared from the dog isolate T-58 was the most reactive. The overall results thus indicated that the T-58 lysate was the optimal reagent when used to detect antibody with the Sure-Blue Reserve substrate. Our laboratory is continuing to studyB. dermatitidisantigen and substrate combinations for the reliable immunodiagnosis of blastomycosis in humans and animals.

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