scholarly journals Blastomyces dermatitidis: Antibody Detection in Sera from Dogs with Blastomycosis with Yeast Lysate Antigens Produced from Human and Dog Isolates

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Katie Mondada ◽  
Jessie Fullmer ◽  
Eric Hungerford ◽  
Katrina Novack ◽  
Kristen Vickers ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Systemic Disease ◽  
Human Isolate ◽  
Antibody Detection ◽  
Blastomyces Dermatitidis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Yeast Lysate ◽  
Lysate Antigens

Dogs are common hosts to the fungal organismBlastomyces dermatitidis, which causes the systemic disease blastomycosis. The goal of our study was to compare the reactivity of twoB. dermatitidisyeast lysate antigens prepared from dog isolates (ERC-2, Wisconsin; T-58, Tennessee) and two lysate antigens prepared from human isolates (B5931 and B5896, Minnesota) against 48 serum specimens from dogs with confirmed blastomycosis using the indirect enzyme-linked immunosorbent assay (ELISA). Secondarily, we used three different ELISA substrates (Ultra TMB: A, SureBlue: B, and SureBlue Reserve: C) to compare the effectiveness of each substrate. Mean absorbance values ranged from 0.446 (B) to 0.651 (C) for the B5931 antigen and from 0.393 (B) to 0.540 (C) for the ERC-2 antigen in Trial 1. In Trial 2, the absorbance values ranged from 0.628 (B) to 0.909 (A) for the B5896 antigen and from 0.828 (B) to 1.375 (C) for the T-58 antigen. In Trial 1, the lysate antigen prepared from the human isolate B5931 exhibited the highest absorbance value and in Trial 2 the lysate prepared from the dog isolate T-58 was the most reactive. The overall results thus indicated that the T-58 lysate was the optimal reagent when used to detect antibody with the Sure-Blue Reserve substrate. Our laboratory is continuing to studyB. dermatitidisantigen and substrate combinations for the reliable immunodiagnosis of blastomycosis in humans and animals.

scholarly journals Blastomyces dermatitidisYeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Alex R. Boyd ◽  
Jamie L. VanDyke ◽  
Gene M. Scalarone
Keyword(s):  
Comparative Study ◽  
Fungal Infection ◽  
Antibody Detection ◽  
Blastomyces Dermatitidis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diagnostic Challenge ◽  
Systemic Fungal Infection ◽  
Yeast Lysate ◽  
The Individual ◽  
Lysate Antigens

The systemic fungal infection, blastomycosis, which infects both humans and animals has presented a diagnostic challenge for clinicians for many years. The aim of this study was to evaluate the diagnostic sensitivity ofBlastomyces dermatitidisyeast lysate antigens with respect to antibody detection in dogs with blastomycosis. Lysate antigens were prepared fromB. dermatitidisisolates T-58 and T-66 (dogs, Tennessee) and WI-R and WI-J (dogs, Wisconsin). Based on results obtained from a preliminary comparative study, five combinations of these isolates and one individual isolate were tested against 92 serum specimens from dogs with culture-proven or histologically-confirmed blastomycosis, using the indirect enzyme-linked immunosorbent assay (ELISA). Mean absorbance values obtained from the sera ranged from 0.905 with the individual T-58 antigen to 1.760 using an antigen combination (T-58 + T-66 + WI-R). All of the 6 antigenic preparations were able to detect antibody in the serum specimens, but the antigen combinations detected antibody to a higher degree than the individual antigen. This study provides evidence that combinations of the yeast lysate reagents seem to be more efficacious for antibody detection in dog sera, but our laboratory is continuing to evaluate antigen lysate combinations for detection of antibodies in blastomycosis.

scholarly journals Comparison of Antibody Detection with Yeast Lysate Antigens Prepared from Blastomyces dermatitidis Dog Isolates from Wisconsin and Tennessee

2013 ◽  
Vol 03 (01) ◽  
pp. 67-72 ◽  
Author(s):  
Jessica J. Roberts ◽  
Michael V. Madrid ◽  
Lindsy Dickerson ◽  
Bradi Hutchison ◽  
Gene M. Scalarone
Keyword(s):  
Antibody Detection ◽  
Blastomyces Dermatitidis ◽  
Yeast Lysate ◽  
Lysate Antigens

Detection and Titration of Human Herpesvirus-8–Specific Antibodies in Sera From Blood Donors, Acquired Immunodeficiency Syndrome Patients, and Kaposi's Sarcoma Patients Using a Whole Virus Enzyme-Linked Immunosorbent Assay

Blood ◽  
1998 ◽  
Vol 92 (1) ◽  
pp. 53-58 ◽  
Author(s):  
Louise G. Chatlynne ◽  
William Lapps ◽  
Michael Handy ◽  
Yao Q. Huang ◽  
Rizwan Masood ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Kaposi's Sarcoma ◽  
Blood Donors ◽  
Human Herpesvirus ◽  
Kaposi’S Sarcoma ◽  
Antibody Detection ◽  
Human Herpesvirus 8 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Herpesvirus 8

A human herpesvirus-8 (HHV-8) enzyme-linked immunosorbent assay (ELISA) with a whole virus lysate as antigen was developed and used to measure the seroprevalence rate and levels of IgG antibodies to HHV-8 in sera/plasma of various patient groups and blood donors. The virus antigen was prepared from the KS-1 cell line, which produces lytic virus, and therefore contains a broad array of viral proteins. Seroprevalence studies using this ELISA showed the following: 10 of 91 blood donors (11%) had an average HHV-8 antibody titer of 118; 67 of 72 (93%) classic Kaposi's sarcoma (KS) patients were positive with an average titer of 14,111; and 57 of 62 (92%) KS/human immunodeficiency virus (HIV) patients were positive with an average titer of 4,000. A study on a very limited number of serial serum samples from patients before and after diagnosis with KS showed highly elevated antibody titers to HHV-8 virus after KS lesions developed. Preliminary data show that 50% of the sera from HIV-1+ homosexual patients contain IgG antibodies to HHV-8 suggesting that this population is at high risk for developing KS. Antibody results correlated well with the confirmatory immunofluorescent assays (IFA) using KS-1 cells as the substrate. This HHV-8 IgG antibody detection ELISA is sensitive and specific and does not cross-react with Epstein-Barr virus (EBV) or other human herpesviruses. The results of this HHV-8 antibody survey suggest that this rapid ELISA assay can be used to screen large numbers of sera to find those at risk for developing KS.

A study of the correlation between Coxiella burnetii seropositivity and abortions in sheep in Eastern and Southeastern Turkey

2016 ◽  
Author(s):  
Ayse Kilic ◽  
Hakan Kalender
Keyword(s):  
Immunosorbent Assay ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Eastern Anatolia ◽  
Obligate Intracellular Bacterium ◽  
Serum Samples ◽  
Obligate Intracellular ◽  
Elisa Kit

Q fever is a zoonotic disease that occurs worldwide and is caused by the obligate intracellular bacterium Coxiella burnetii. Infected animals are usually asymptomatic, but infection can cause abortion and stillbirth in ruminants. The main purpose of this study was to evaluate prevalance of Coxiella burnetii infection in aborted and nonaborted sheep serum samples in Eastern Anatolia region by using enzyme-linked immunosorbent assay (ELISA). The determine of prevalance in sheep flocks from four provinces (Elazig, Malatya, Tunceli, Bitlis) and tested for anti-C.burnetii antibody detection, by means of Chekit Q fever Elisa kit. 350 serum samples obtained from flocks belonging aborted sheep showed that a total of 56 (16%) were detected seropositivity, whereas 171 serum samples obtained from nonaborted sheep flocks in 13 of the 171 (7.60%) for C.burnetii in seropositivity were observed. Coxiellosis should be considered an important cause of sheep with abortion history and nonaborted in Elazig and neighboring provinces.

scholarly journals Blastomyces dermatitidis: Stability studies on different yeast lysate antigens

2013 ◽  
Vol 03 (03) ◽  
pp. 98-102 ◽  
Author(s):  
Tiffany R. Allison ◽  
Joshua C. Wright ◽  
Gene M. Scalarone
Keyword(s):  
Blastomyces Dermatitidis ◽  
Stability Studies ◽  
Yeast Lysate ◽  
Lysate Antigens

scholarly journals Comparisons of Anti-dsDNA Antibody Detection Methods by Chemiluminescent Immunoassay and Enzyme-Linked Immunosorbent Assay in Systemic Lupus Erythematosus

Diagnostics ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1940
Author(s):  
Huang-Chen Chang ◽  
Yen-Ching Wu ◽  
Jun-Peng Chen ◽  
Yi-Da Wu ◽  
Wen-Nan Huang ◽  
...  
Keyword(s):  
Disease Activity ◽  
Immunosorbent Assay ◽  
Lupus Erythematosus ◽  
Predictive Power ◽  
Detection Methods ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dsdna Antibody ◽  
Chemiluminescent Immunoassay ◽  
Diagnostic Power

This study aimed to compare the test results of anti-double-stranded DNA (anti-dsDNA) antibodies obtained using chemiluminescent immunoassay (CIA) and enzyme-linked immunosorbent assay (ELISA), and investigate predictors of inconsistent results. This retrospective study included 502 patients who underwent CIA and ELISA to determine their anti-dsDNA antibody values within a year. We compared the diagnostic power for SLE, disease activity, and predictive power for lupus nephritis (LN). A multivariate analysis was performed to determine the predictors of inconsistencies. CIA and ELISA were moderately correlated in terms of their consistency (Cronbach’s α = 0.571), and yielded comparably favorable results in terms of SLE diagnostic power and SLE disease activity. However, if the patient had LN, CIA displayed higher predictive power than ELISA (0.620 vs. 0.555, p = 0.026). Compared with the CIA/ELISA double-positive group, the inconsistent group had lower anti-C1q circulating immune complexes (CIC) antibody values (OR: 0.42, 95% CI: 0.18–0.94, p = 0.036), and lower SLEDAI scores (≥4) (OR: 0.33, 95% CI: 0.14–0.79, p = 0.013). Anti-dsDNA antibody detection with CIA exhibited higher predictability for diagnosing LN than did ELISA. In the event of inconsistencies between anti-dsDNA methods, SLE disease activity and CIC test values should be considered simultaneously.

scholarly journals Enzyme linked immunosorbent assay: determination of anti-adenovirus antibodies in an infant population

1989 ◽  
Vol 31 (5) ◽  
pp. 336-340 ◽  
Author(s):  
Adriana Weinberg ◽  
Maria Cristina D. S. Fink ◽  
Sueko Takimoto ◽  
Maria Akiko Ishida ◽  
Maria Cândida O. Souza
Keyword(s):  
Immunosorbent Assay ◽  
Gold Standard ◽  
Maternal Antibodies ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay

In order to define an accurate assay for anti-adenovirus antibody detection, a recently developed ELISA was compared with IFA and CF. On 58 sera, the ELISA was more sensitive than both CF and IFA, which showed relative sensitivities of 63% and 94%, respectively. It was not possible to determine the exact specificity of the tests because of the lack of a gold standard. Furthermore, the ELISA was used to define the prevalence of adenovirus antibodies in 116 infants between 1 and 24 months old (mean 7.28). The data showed that maternal antibodies waned by the age of 5 to 6 months and that more than 80% of the children had been infected by adenoviruses by the age of 10 months.

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