scholarly journals Isolation and Identification of Shoot-Tip Associated Endophytic Bacteria from Banana cv. Grand Naine and Testing for Antagonistic Activity against <i>Fusarium oxysporum</i> f. sp. <i>cubense</i>

2015 ◽  
Vol 06 (07) ◽  
pp. 943-954 ◽  
Author(s):  
Aparna Chandra Sekhar ◽  
Pious Thomas
Keyword(s):  
Fusarium Oxysporum ◽  
Endophytic Bacteria ◽  
Antagonistic Activity ◽  
Shoot Tip ◽  
Isolation And Identification

scholarly journals Unraveling the Metabolite Signature of Endophytic Bacillus velezensis Strain Showing Defense Response Towards Fusarium oxysporum

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 683
Author(s):  
Yang Cao ◽  
Wei Ding ◽  
Chang Liu
Keyword(s):  
Fusarium Oxysporum ◽  
Inhibitory Activity ◽  
Defense Response ◽  
Endophytic Bacteria ◽  
Rice Seedling ◽  
Seedling Blight ◽  
Bacillus Velezensis ◽  
Secondary Bile Acid ◽  
Pathogen Inhibition

Seedling blight, caused by the fungus Fusarium oxysporum, significantly lowers rice production globally. Earlier reports have opined that endophytic bacteria strains could be possible biocontrol agents, but the mechanistic actions involved are still unclear. Therefore, this study aimed to isolate the endophytic bacteria with high inhibitory activity and elucidate its possible mechanisms for inducing resistance by metabolomics. The results showed that mdj-36 had the strongest in vitro pathogen inhibition of F. oxysporum, while mdj-34 displayed the lowest inhibitory activity identified as Bacillus velezensis strains. Metabolomic analyses demonstrated that B. velezensis mdj-36 growth medium could produce higher organic acids, terpenes, and diterpene than B. velezensis mdj-34. Further investigation revealed that ‘secondary bile acid biosynthesis’ and ‘glycerophospholipid metabolism’ pathways played essential roles in defense response towards F. oxysporum. This study’s findings provide a credible theoretical basis for the possible use of the B. velezensis strain against rice seedling blight.

scholarly journals Antagonistic activity of endophytic bacteria isolated from weed plant against stem end rot pathogen of pitaya in Vietnam

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
The Anh Luu ◽  
Quyet Tien Phi ◽  
Thi Thu Hang Nguyen ◽  
Mai Van Dinh ◽  
Bich Ngoc Pham ◽  
...  
Keyword(s):  
Endophytic Bacteria ◽  
Indole Acetic Acid ◽  
Pathogenic Fungi ◽  
Antagonistic Activity ◽  
Biofilm Production ◽  
Weed Plant ◽  
Seedling Biomass ◽  
Rot Disease ◽  
Positive Results

Abstract Background Fungal stem end rot disease of pitaya caused by Alternaria alternata is one of the most destructive diseases in Binh Thuan province, Vietnam. This study aimed to assess the antagonistic effects of some endophytic bacteria isolated from the weed plant (Echinochloa colonum) against A. alternata. Results A total of 19 endophytic bacteria were isolated and 5 of them presented in vitro antagonistic activity against A. alternata. Of five, strain EC80 significantly inhibited the pathogenic growth with a mean inhibition diameter of 11.88 ± 0.08 mm, while the other four (C79, EC83, EC90, and EC97) showed a weak inhibition. Interestingly, the combination of EC79 and EC80 reduced more biomass of pathogenic fungi than the single one did. EC79 showed positive results for amylase, indole acetic acid (IAA), and biofilm production, whereas EC80 presented positive capabilities for IAA and biofilm production and a negative one for amylase production. In addition, the combined filtrate of EC79 and EC80 presented non-antifungal activity on biocontrol tests in vitro, indicating that bacteria cells played a role in defending against the pathogen. Moreover, both isolates EC79 and EC80 significantly increased seedling biomass than the control. Conclusions The results suggest that those two strains in combination had the potential to be used as a biocontrol agent against A. alternata. More studies should be done in the future to evaluate their efficiency under the field conditions.

scholarly journals Antagonistic Activity of Pseudomonas Fluorescens Against Fusarium Oxysporum f. sp. Nievum Isolated from Soil Samples in Palestine

2017 ◽  
Vol 6 (2) ◽  
pp. 1 ◽  
Author(s):  
Mazen Salman ◽  
Nabil Shahin ◽  
Nawaf Abu-Khalaf ◽  
Mohammad Jawabrih ◽  
Basima Abu Rumaileh ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Pseudomonas Fluorescens ◽  
Field Experiments ◽  
Antagonistic Activity ◽  
Arab Countries ◽  
Local Market ◽  
Control Mechanisms ◽  
The Past ◽  
Normal Seedling ◽  
Severe Infections

Watermelon is an important summer crop in Palestine, for several decades filling the needs of local market and some Arab countries. The yield of watermelon decreased dramatically in recent years due to severe infections with the soil borne fungal pathogen Fusarium oxysporum f. sp. niveum (FON). Soil fumigation with methyl bromide was commonly applied by Palestinian farmers until it was recently legally banned. Different control mechanisms were not feasible to overcome problems caused by the disease resulting in decreased watermelon cultivation in Palestine for the past 30 years. In this work, we have experimentally shown that Pseudomonas fluorescens was efficient in controlling FON infection and allowing normal seedling growth of both the root and shoot systems. Field experiments are necessary to further confirm the efficacy of biocontrol application.

scholarly journals Screening of endophytes from rubber trees (hevea brasiliensis) for biological control of Corticium salmonicolor

2020 ◽  
Vol 8 (2) ◽  
pp. 38-44
Author(s):  
Nguyen Van Minh ◽  
Mai Huu Phuc ◽  
Duong Nhat Linh ◽  
Tran Thi A Ni ◽  
Tran Kien Duc ◽  
...  
Keyword(s):  
Biological Control ◽  
Hevea Brasiliensis ◽  
Endophytic Bacteria ◽  
Rubber Tree ◽  
Antagonistic Activity ◽  
Ho Chi Minh City ◽  
Viet Nam ◽  
Dual Culture ◽  
Living Tissue ◽  
Tissue Samples

28 leaves and living-tissue samples of rubber tree (Hevea brasiliensis) were collected from Ho Chi Minh City, Binh Phuoc province and Binh Duong province (Viet Nam). We isolated and screened endophytes that have potential application as agents for biocontrol of Corticium salmonicolor, the agent of Pink Disease in rubber trees. As a result, 21 strains of endophytic bacteria and 14 strains of endophytic fungi were isolated. Antagonistic activity of the endophytes towards C. salmonicolor was checked by using a dual culture. Testing results showed that: T9, T15 and T16 strains have inhibited C. salmonicolor. T9 and T16 strains showed result that 100% of inhibiting C. salmonicolor at the concentration of 1:1. In the test of ability to kill C. salmonicolor, T9 and T16 strains showed that they could kill C. salmonicolor after 3 sprays of bacterial filtrate. T9 and T6 strains, which were identified by biochemical methods, have similar characteristics to Bacillus thuringiensis.

scholarly journals ISOLASI DAN IDENTIFIKASI PATOGEN GETAH KUNING MANGGIS MELALUI PENDEKATAN POSTULAT KOCH DAN ANALISIS SECARA MOLEKULER

2014 ◽  
Vol 14 (2) ◽  
pp. 142-151
Author(s):  
I Ketut Suada ◽  
Ni Wayan Suniti
Keyword(s):  
Fusarium Oxysporum ◽  
Molecular Analysis ◽  
Fruit Quality ◽  
Irrigation Treatment ◽  
Causal Agent ◽  
Isolation And Identification ◽  
Fungus Species ◽  
Cell Turgor ◽  
Bitter Fruit

Isolation and identification of mangosteen yellow latex pathogen through Koch’s Postulate application and molecular analysis. The yellow latex disease decreases fruit quality of mangosteen because exogenic latex causes dirty fruit and the endogenic latex makes the bitter fruit taste and does not deserve to be consumed. The causal agent of the disease caused phusilogical disorder so that cells break due to unstability of cell turgor, the drift irrigation treatment was able to decrease the disease of 35.22%, therefore the causal agent could be microbe that promote the yellow latex. This research was aimed to find the yellow latex pathogens with its characteristics. The Koch Postulate was apllied to isolate and proved the microbes associated to the yellow latex and molecular analysis was constructed subsequently to find the microbe species.  The result of the research proved that there were three fungus species as the biotic agent responsible to the disease. The fungus were Verticillium albo-atrum with the highest pathogenecity ( 74.87%), followed by Fusarium oxysporum ( 70.15%), and Pestalotia macrotricha (20.32%).

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