Isolation and Identification of Fungi from the Animal Dung in Thamar - Yemen and Estimation of the Antagonistic Activity of Papulospora sp. against Fusarium Oxysporum

2014 ◽  
Vol 1 (2) ◽  
pp. 22-26
Author(s):  
M.A. Moghalles ◽  
S. M. Al-Bader
Keyword(s):  
Fusarium Oxysporum ◽  
Antagonistic Activity ◽  
Isolation And Identification ◽  
Animal Dung ◽  
Identification Of Fungi

scholarly journals ISOLASI DAN IDENTIFIKASI CENDAWAN TERBAWA BENIH KAKAO HIBRIDA

2020 ◽  
Vol 18 (1) ◽  
pp. 40
Author(s):  
BAHARUDIN BAHARUDIN ◽  
AGUS PURWANTARA ◽  
SATRIYAS ILYAS ◽  
MOHAMAD RAHMAD SUHARTANTO
Keyword(s):  
Fusarium Oxysporum ◽  
Theobroma Cacao ◽  
Potato Dextrose Agar ◽  
Aspergillus Ochraceus ◽  
Fusarium Spp ◽  
Isolation And Identification ◽  
Randomized Design ◽  
Seedborne Fungi ◽  
Identification Of Fungi ◽  
Fungi Infection

<p>ABSTRAK<br />Benih merupakan komponen dasar dalam menentukan produktivitas<br />tanaman kakao. Benih yang sehat dapat merupakan faktor penting dalam<br />menentukan keberhasilan produktivitas kakao. Benih kakao mempunyai<br />kadar air cukup tinggi sehingga berpotensi terinfeksi cendawan, yang<br />dapat menurunkan mutu benih dan produksi kakao. Penelitian bertujuan<br />untuk mengisolasi dan mengidentifikasi beberapa cendawan terbawa benih<br />pada kakao hibrida. Penelitian dilakukan di Kebun Induk Benih Pusat<br />Penelitian Kopi dan Kakao Indonesia, Jember, Laboratorium Mikro-<br />biologi, Balai Penelitian Bioteknologi Perkebunan Indonesia, dan<br />Laboratorium Pengendalian Hayati IPB Bogor pada bulan Juni sampai<br />Oktober 2008. Penelitian menggunakan benih kakao hibrida dari hasil<br />persilangan buatan antar TSH 858 dengan Sca 6, dan percobaan disusun<br />dengan rancangan acak lengkap dengan 3 ulangan. Benih ditumbuhkan<br />pada 3 media, yaitu water agar (WA), potato dextrose agar (PDA), dan<br />kertas saring (KS). Tingkat infeksi pada benih diamati setiap hari dan<br />dianalisis dengan analisis sidik ragam dan dilanjutkan dengan uji selang<br />berganda Duncan. Cendawan diisolasi, dibiakkan, dimurnikan, dan<br />diidentifikasi dengan menggunakan buku kunci identifikasi. Tingkat<br />infeksi cendawan terbawa benih kakao hibrida tertinggi terdapat pada hari<br />keempat (35,00%) dan kelima (51,67%) pada media PDA. Sebanyak 13<br />spesies cendawan terbawa benih kakao hibrida berhasil diidentifikasi<br />dengan menggunakan media WA dan PDA, serta 8 spesies cendawan<br />dengan media KS. Ke-13 cendawan terbawa benih yang ditemukan sangat<br />berpotensi menurunkan mutu fisiologis benih dan produktivitas kakao.<br />Cendawan tersebut perlu diuji lebih lanjut karena masing-masing memiliki<br />sifat-sifat patogenik, saprofitik, atau antagonistis terhadap cendawan lain<br />pada benih kakao. Cendawan terbawa benih kakao hibrida paling dominan<br />adalah Aspergillus spp., Penicillium chrysogenium, Coletotrichum<br />acutatum, Curvularia geniculata, dan Fusarium spp. Cendawan-cendawan<br />yang diduga berbahaya adalah Aspergillus spp., Coletotrichum acutatum,<br />Curvularia  geniculata,  Fusarium  spp.,  Phoma  glomerata,  dan<br />Macrophoma sp., dan yang diduga bersifat patogenik adalah Aspergillus<br />flavus, Aspergillus ochraceus, Cladosporium herbanum, Curvularia<br />geniculata, Fusarium oxysporum, Phoma glomerata, dan Macrophoma sp.</p><p>Kata kunci : Theobroma cacao, benih hibrida, infeksi cendawan, media<br />tanam</p><p>Isolation and Identification of Fungi on Hybrid Cacao Seeds</p><p>ABSTRACT<br />Seed is the basic component influencing the productivity of cacao<br />plantation. Healthy seed is the most important factor in determining the<br />success of cacao productivity. Moisture content of cacao seeds is quite<br />high potentially to cause fungi infection, which can further reduce seed<br />quality and cacao production. The research aimed at isolating and<br />identifying several seedborne fungi on hybrid cacao. The study was<br />conducted at main nursery of Indonesian Coffee and Cocoa Research<br />Institute Jember, Laboratory of Microbiology, Indonesian Biotechnology<br />Research Institute for Estate Crops, and the Laboratory for Biological<br />Control of IPB Bogor from June to October 2008. Research used hybrid<br />cacao seeds derived from crossing between TSH 858 x SCA 6, and the<br />experiment was arranged using completely randomized design with three<br />replicates. Cacao seeds were grown on three media, i.e. water agar (WA),<br />potato dextrose agar (PDA), and filter paper (KS). Infection rates on the<br />seedlings were observed every day and analyzed using ANOVA followed<br />by Duncan's multiple regression test (DMRT). Fungi were isolated,<br />cultured, purified, and identified using the identification keys. The highest<br />rate of seedborn fungal infection occured on fourth (35.00%) and fifth<br />(51.67%) days on PDA media. A total of 13 species of seedborn fungi on<br />hybrid cocoa were identified by using WA and PDA media, as well as 8<br />other species by using KS. The 13 seedborne fungi potentially reduce seed<br />physiological quality and cacao productivity. These fungi need to be<br />further tested because each has its own pathogenic, saprophytic, or<br />antagonistic properties towards other fungi on cacao seeds. Predominant<br />seedborn fungi on hybrid cacao were Aspergillus spp., Penicillium<br />chrysogenium, Coletotrichum acutatum, Curvularia geniculata, and<br />Fusarium spp. The fungi suspected harmful were Aspergillus spp.,<br />Coletotrichum acutatum, Curvularia geniculata, Fusarium spp., Phoma<br />glomerata, and Macrophoma sp., and those suspected pathogenic were<br />Aspergillus flavus, Aspergillus ochraceus, Cladosporium herbanum,<br />Curvularia geniculata, Fusarium oxysporum, Phoma glomerata, and<br />Macrophoma sp.<br />Key words : Theobroma cacao, fungi infection, hybrid seed, growing<br />media</p>

scholarly journals Antagonistic Activity of Pseudomonas Fluorescens Against Fusarium Oxysporum f. sp. Nievum Isolated from Soil Samples in Palestine

2017 ◽  
Vol 6 (2) ◽  
pp. 1 ◽  
Author(s):  
Mazen Salman ◽  
Nabil Shahin ◽  
Nawaf Abu-Khalaf ◽  
Mohammad Jawabrih ◽  
Basima Abu Rumaileh ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Pseudomonas Fluorescens ◽  
Field Experiments ◽  
Antagonistic Activity ◽  
Arab Countries ◽  
Local Market ◽  
Control Mechanisms ◽  
The Past ◽  
Normal Seedling ◽  
Severe Infections

Watermelon is an important summer crop in Palestine, for several decades filling the needs of local market and some Arab countries. The yield of watermelon decreased dramatically in recent years due to severe infections with the soil borne fungal pathogen Fusarium oxysporum f. sp. niveum (FON). Soil fumigation with methyl bromide was commonly applied by Palestinian farmers until it was recently legally banned. Different control mechanisms were not feasible to overcome problems caused by the disease resulting in decreased watermelon cultivation in Palestine for the past 30 years. In this work, we have experimentally shown that Pseudomonas fluorescens was efficient in controlling FON infection and allowing normal seedling growth of both the root and shoot systems. Field experiments are necessary to further confirm the efficacy of biocontrol application.

scholarly journals Selection, isolation, and identification of fungi for bioherbicide production

2017 ◽  
Vol 48 (1) ◽  
pp. 101-108 ◽  
Author(s):  
Angélica Rossana Castro de Souza ◽  
Daiana Bortoluzzi Baldoni ◽  
Jessica Lima ◽  
Vitória Porto ◽  
Camila Marcuz ◽  
...  
Keyword(s):  
Isolation And Identification ◽  
Identification Of Fungi

scholarly journals Isolation and identification of fungi associated with some Libyan foods

2015 ◽  
Vol 9 (7) ◽  
pp. 406-410 ◽  
Author(s):  
Alkenz S ◽  
A Sassi A ◽  
S Abugnah Y ◽  
B Alryani M
Keyword(s):  
Isolation And Identification ◽  
Identification Of Fungi

scholarly journals ISOLASI DAN IDENTIFIKASI PATOGEN GETAH KUNING MANGGIS MELALUI PENDEKATAN POSTULAT KOCH DAN ANALISIS SECARA MOLEKULER

2014 ◽  
Vol 14 (2) ◽  
pp. 142-151
Author(s):  
I Ketut Suada ◽  
Ni Wayan Suniti
Keyword(s):  
Fusarium Oxysporum ◽  
Molecular Analysis ◽  
Fruit Quality ◽  
Irrigation Treatment ◽  
Causal Agent ◽  
Isolation And Identification ◽  
Fungus Species ◽  
Cell Turgor ◽  
Bitter Fruit

Isolation and identification of mangosteen yellow latex pathogen through Koch’s Postulate application and molecular analysis. The yellow latex disease decreases fruit quality of mangosteen because exogenic latex causes dirty fruit and the endogenic latex makes the bitter fruit taste and does not deserve to be consumed. The causal agent of the disease caused phusilogical disorder so that cells break due to unstability of cell turgor, the drift irrigation treatment was able to decrease the disease of 35.22%, therefore the causal agent could be microbe that promote the yellow latex. This research was aimed to find the yellow latex pathogens with its characteristics. The Koch Postulate was apllied to isolate and proved the microbes associated to the yellow latex and molecular analysis was constructed subsequently to find the microbe species.  The result of the research proved that there were three fungus species as the biotic agent responsible to the disease. The fungus were Verticillium albo-atrum with the highest pathogenecity ( 74.87%), followed by Fusarium oxysporum ( 70.15%), and Pestalotia macrotricha (20.32%).

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