scholarly journals An Improved System for Shoot Regeneration from Stem Explants of Lombardy Poplar (Populus nigra L. var. italica Koehne)

2012 ◽  
Vol 03 (09) ◽  
pp. 1181-1186 ◽  
Author(s):  
Kamal Kanti Biswas ◽  
Takeshi Mohri ◽  
Satoshi Kogawara ◽  
Yoshihiro Hase ◽  
Yutaka Oono
Keyword(s):  
Shoot Regeneration ◽  
Populus Nigra ◽  
Stem Explants ◽  
Lombardy Poplar

scholarly journals Explants selection for in vitro propagation of Pachyrhizus erosus L.

2021 ◽  
Vol 13 (1) ◽  
pp. 10844
Author(s):  
Idowu A. OBISESAN ◽  
Ayobola M. A. SAKPERE ◽  
Bamidele J. AMUJOYEGBE ◽  
Michael S. AKINROPO
Keyword(s):  
Shoot Regeneration ◽  
In Vitro Propagation ◽  
Callus Formation ◽  
Leaf Explants ◽  
Stem Explants ◽  
Ms Medium ◽  
Friable Callus ◽  
Pachyrhizus Erosus ◽  
Regeneration Response

Pachyrhizus erosus tuber is rich in protein asides its agronomical value as a legume, but the seeds by which it is propagated have very low viability. This study established sterilization protocol and effect of various concentrations of auxins and cytokinins on callus production and shoot regeneration from explants of P. erosus. Explants and seeds were sterilized using sodiumhypochlorite (NaClO) solution (5, 10 and 15% v/v) for 5 and 10 mins. Nodal, stem and leaf explants from in vitro germinated P. erosus and tuber from field grown plant were sterilized and cultured on Murashige and Skoog (MS) medium (control) and MS combined with different concentrations of auxins (NAA and 2, 4-D) and cytokinin (BA and Kinetin) and the cultured explants were monitored in terms of degree of callus formation, morphology and colour of callus and also for shoot induction. The results showed that seeds of P. erosus sterilized with 10% NaClO solution for 10 mins and germinated in vitro is the best way of getting sterile nodal, stem and leaf explants for the in vitro propagation of the plant, while tuber explants could be sterilized with 15% NaClO for 10 minutes. Nodal explants inoculated in MS medium supplemented with 1.0 mg/L BA gave the highest shoot regeneration response, while stem explants inoculated on MS medium supplemented with 1.0 mg/L BA and 0.5 mg/L NAA also gave the highest amount of friable callus. The study concluded that in vitro germinated seeds were the best way of getting explant for P. erosus.

scholarly journals Molecular identification of a phytoplasma naturally infecting Populus nigra L. cv. Italica trees in Croatia

2002 ◽  
Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽  
pp. S28-S30
Author(s):  
M. Šeruga ◽  
D. Škorić ◽  
S. Botti ◽  
S. Paltrinieri ◽  
N. Juretić ◽  
...  
Keyword(s):  
16S Rdna ◽  
Nested Pcr ◽  
Late Summer ◽  
Populus Nigra ◽  
Aster Yellows ◽  
Leaf Yellowing ◽  
Pcr Assays ◽  
Nested Pcr Assays ◽  
Zagreb Area ◽  
Lombardy Poplar

Leaf and branch samples of 10 Populus nigra L. cv. Italica trees were collected from the urban Zagreb area in late summer/autumn 2001. One of the trees exhibited leaf yellowing, overall sparse foliage, stunting and decline. Phytoplasma 16S rDNA was amplified in direct and nested PCR assays using universal and specific phytoplasma primer pairs, from nucleic acids extracted by two different procedures. Strong amplification signals were observed in samples from symptomatic Lombardy poplar as well as in samples from 4 of the asymptomatic trees. RFLP analyses of amplicons showed patterns characteristic of the phytoplasmas belonging to the Aster yellows group (16SrI). This is the first report of a phytoplasma naturally infecting poplar in Croatia.

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