scholarly journals Quantitative Analysis of ATP Sulfurylase and Selenocysteine Methyltransferase Gene Expression in Different Organs of Tea Plant (<i>Camellia sinensis</i>)

2012 ◽  
Vol 03 (01) ◽  
pp. 51-59 ◽  
Author(s):  
Shaoqiang Tao ◽  
Juan Li ◽  
Xungang Gu ◽  
Yanan Wang ◽  
Qiang Xia ◽  
...  
Keyword(s):  
Gene Expression ◽  
Quantitative Analysis ◽  
Camellia Sinensis ◽  
Tea Plant ◽  
Atp Sulfurylase ◽  
Methyltransferase Gene ◽  
Selenocysteine Methyltransferase

scholarly journals Effect of fluoride treatment on gene expression in tea plant (Camellia sinensis)

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Qing-Sheng Li ◽  
Xiao-Ming Lin ◽  
Ru-Ying Qiao ◽  
Xin-Qiang Zheng ◽  
Jian-Liang Lu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Camellia Sinensis ◽  
Tea Plant ◽  
Fluoride Treatment

scholarly journals Selenium induced selenocysteine methyltransferase gene expression and antioxidant enzyme activities in Astragalus chrysochlorus

2016 ◽  
Vol 75 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Özgür Çakir ◽  
Neslihan Turgut-Kara ◽  
Şule Ari
Keyword(s):  
Gene Expression ◽  
Biological Activities ◽  
Folk Medicine ◽  
Transcript Level ◽  
Antioxidant Enzyme Activities ◽  
Gene Transcript ◽  
Methyltransferase Gene ◽  
Selenocysteine Methyltransferase ◽  
Callus Tissues ◽  
High Level

Abstract Astragalus sp. are used in folk medicine because of their biological activities and are known for the ability to accumulate high levels of selenium (Se). The purpose of this study was to explore gene expression of selenocysteine methyltransferase (SMT), responsible for forming MeSeCys, and activities of ascorbate peroxidase (APX), peroxidase (POX), catalase (CAT) and glutathione reductase (GR) enzymes in callus tissues of Astragalus chrysochlorus growing in different Se-containing media. Quantitative real-time polymerase chain reaction assay was done for quantification of SMT gene transcript and it was normalized to actin gene. It was found that transcript level of callus tissues grown at 5.2 μM and 26.4 μM Se-enriched media was lower than that of the control callus. In contrast, a high level of Se (132.3 μM) in the medium caused an approximately 4.26 times higher level of SMT transcript in callus than the control. APX, POX, CAT and GR enzymes were all effected by different Se concentrations. While POX and APX activities were higher then control, CAT and GR activities decreased. These results show that an increase of SMT gene expression led to a rise in APX and POX, but a suppression of CAT and GR enzymes activities in Astragalus chrysochlorus. This suggests that Se could be involved in the antioxidant metabolism in Astragalus chrysochlorus.

scholarly journals Evaluation and Selection of Suitable qRT-PCR Reference Genes for Light Responses in Tea Plant (Camellia Sinensis)

2020 ◽  
Author(s):  
Jiaxin Wang ◽  
Qianhui Tang ◽  
Kang Sun ◽  
Liang Zeng ◽  
Zhijun Wu
Keyword(s):  
Gene Expression ◽  
Light Intensity ◽  
Camellia Sinensis ◽  
Reference Genes ◽  
Light Quality ◽  
Family Members ◽  
Tea Plant ◽  
Regulation Mechanism ◽  
Qrt Pcr ◽  
Selection Of

Abstract Background: Tea plant (Camellia sinensis) is an important woody economic crop used for processing leaf-type beverages. Tea has been proved to be beneficial to human health because it is rich in tea polyphenols and other active ingredients. Numerous studies have shown that light is a necessary environmental condition to control the growth and metabolism of C. sinensis. Gene expression experiments are always performed to explore the transcriptional regulation mechanism of plants widely based on the technique of quantitative real time polymerase chain reaction (qRT-PCR). The screening and application of reference genes are necessary for the normalization of gene expression under specific conditions. However, the reference genes for systematic analysis of light-induced transcription mechanisms are still not available in C. sinensis.Results: In this research, we identified actin family genes that are always used as reference genes with high frequency and without distinction for various expression experiments in C. sinensis. Six pairs of distinctive primers (corresponding to CsACT1, CsACT2, CsACT(3-4), CsACT(5-6), CsACT(7-8), and CsACT(9-10) genes) were designed to evaluate their expression stability in response to light quality (LQ), light intensity (LI), and photoperiod (PD). Simultaneously, six other family members (CsUBC1, CsCLATHRIN1, CsGAPDH, CsTBP, CsTIP41, and CseIF-4α) of C. sinensis commonly used as reference genes were also investigated. The stability rankings of gene expression were calculated by the statistical algorithms of geNorm, BestKeeper, NormFinder, and RefFinder softwares. Conclusions: CsACT(5-6), CsTIP41, and CsACT(3-4) were the most stable genes for light quality (LQ), light intensity (LI), and photoperiod (PD) treatments, respectively. This study provides a basis for the selection of reference genes for future research on the transcription mechanism of light response in C. sinensis. Moreover, the analysis of actin family members of C. sinensis will help to understand the individual transcription mechanism of housekeeping family.

Expression for Caffeine Biosynthesis and Related Enzymes in Camellia sinensis

2010 ◽  
Vol 65 (3-4) ◽  
pp. 245-256 ◽  
Author(s):  
Misako Kato ◽  
Naoko Kitao ◽  
Mariko Ishida ◽  
Hanayo Morimoto ◽  
Fumi Irino ◽  
...  
Keyword(s):  
Gene Expression ◽  
Camellia Sinensis ◽  
Heavy Metal Stress ◽  
Tea Plant ◽  
Purine Base ◽  
Caffeine Synthase ◽  
Purine Alkaloid ◽  
Young Leaves ◽  
Caffeine Biosynthesis ◽  
High Concentrations

Caffeine (1,3,7-trimethylxanthine) is a purine alkaloid that is present in high concentrations in the tea plant Camellia sinensis. Caffeine synthase (CS, EC 2.1.1.160) catalyzes the S-adenosyl-L-methionine-dependent N-3- and N-1-methylation of the purine base to form caffeine, the last step in the purine alkaloid biosynthetic pathway. We studied the expression profile of the tea caffeine synthase (TCS) gene in developing leaves and flowers by means of northern blot analysis, and compared it with those of phenylalanine ammonia lyase (PAL, EC 4.3.1.5), chalcone synthase (CHS, EC 2.3.1.74), and S-adenosyl-L-methionine synthase (SAMS, EC 2.5.1.6). The amount of TCS transcripts was highest in young leaves and declined markedly during leaf development, whereas it remained constant throughout the development of the flower. Environmental stresses other than heavy metal stress and plant hormone treatments had no effect on the expression of TCS genes, unlike the other three genes. Drought stress suppressed TCS gene expression in leaves, and the expression pattern mirrored that of the dehydrin gene. The amounts of TCS transcripts increased slightly on supply of a nitrogen source. We discuss the regulation of TCS gene expression

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