scholarly journals RP-HPLC Method for the Simultaneous Determination of Lisinopril and NSAIDs in API, Pharmaceutical Formulations and Human Serum

2012 ◽  
Vol 03 (02) ◽  
pp. 147-152 ◽  
Author(s):  
Najma Sultana ◽  
M. Saeed Arayne ◽  
Rubina Siddiqui ◽  
Safila Naveed
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Rp Hplc

Simultaneous Determination of Chloroquine and Pyrimethamine with Cetirizine in an Active Form and Human Serum by RP-HPLC

2021 ◽  
Author(s):  
Hina Shamshad ◽  
Ali Sayqal ◽  
Jahan Zeb ◽  
Agha Zeeshan Mirza
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Active Form ◽  
Internal Standard ◽  
Hplc Method ◽  
Serum Samples ◽  
Rp Hplc ◽  
Cetirizine Hydrochloride ◽  
Bulk Drug

Abstract A simple, accurate and precise RP-HPLC method was developed for the simultaneous determination of chloroquine, pyrimethamine and cetirizine hydrochloride concentrations in bulk drug and human serum. The assay was performed using a mobile phase of methanol: water (70:30) at pH of 2.8 ± 0.05 on the Purospher C-18 column with UV detection at 230 nm and rosuvastatin used as an internal standard. The retention times observed for chloroquine, pyrimethamine and cetirizine hydrochloride were 3.5, 2.5 and 5.5 minutes, respectively. The method was found to be specific for the assayed drugs showing a linear response in the concentration range of 1–100 μg mL−1 with coefficients of determination values of (r = 0.999). The method was developed and validated according to ICH guidelines. The method was used to monitor the serum samples and was found to be sensitive for therapeutic purposes, showing the potential to be a useful tool for routine analysis in laboratories.

scholarly journals Application of RP-HPLC method for the simultaneous determination of cetirizine in the presence of quinolones

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Hina Shamshad ◽  
Agha Zeeshan Mirza
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Flow Rate ◽  
Mobile Phase ◽  
Diclofenac Sodium ◽  
Internal Standard ◽  
Hplc Method ◽  
Rp Hplc ◽  
Ich Guidelines

Abstract Background Present work describes a fast, simple, and sensitive procedure for the simultaneous determination of cetirizine in the presence of quinolones using diclofenac sodium as an internal standard. The present work was designed to analyze these compounds in pharmaceutical and clinical labs being economical for use. Results The mobile phase consisted of the simple composition of methanol, acetonitrile, and water in a ratio of 50:20:30 with a pH adjusted to 3.1 at a flow rate of 1 mL min−1. The UV detection was performed at 225 nm. The linearity was assessed over the range of 2.5–50 μg mL−1 for all drugs. The parameters such as accuracy, precision, linearity (>0.999), and sensitivity were satisfactory. Conclusion The method was equally applicable for formulation and human serum with recovery values between 95 and 105%. The results of the method were validated statistically according to ICH guidelines.

scholarly journals A RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF DILTIAZEM AND QUINOLONES IN BULK, FORMULATIONS AND HUMAN SERUM

2009 ◽  
Vol 54 (4) ◽  
Author(s):  
NAJMA SULTANA ◽  
M. SAEED ARAYNE ◽  
NIGHAT SHAFI ◽  
ASIA NAZ ◽  
SHABANA NAZ ◽  
...  
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Hplc Method ◽  
Rp Hplc

scholarly journals Development and Validation of Stability-Indicating HPLC Method for the Simultaneous Determination of Paracetamol, Tizanidine, and Diclofenac in Pharmaceuticals and Human Serum

2011 ◽  
Vol 94 (1) ◽  
pp. 150-158 ◽  
Author(s):  
Farhan Ahmed Siddiqui ◽  
M Saeed Arayne ◽  
Najma Sultana ◽  
Faiza Qureshi
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Ammonium Carbonate ◽  
Linear Regression Analysis ◽  
Pharmaceutical Formulations ◽  
Analysis Data ◽  
Hplc Method ◽  
Hplc Separation ◽  
Development And Validation

Abstract A method is described for the simultaneous determination of paracetamol, tizanidine, and diclofenac in mixtures. The method was based on HPLC separation of the three drugs followed by UV detection at 254 nm. The separation was carried out on a Hypersil ODS, C18 (250 × 4.6 mm id, 10 μm particle size) column using the mobile phase aqueous 0.2% ammonium carbonate–methanol (60 + 40, v/v) at a flow rate of 1 mL/min. The linear regression analysis data were used for the regression curve in the range of 170–10,000 ng/mL for paracetamol, 120–10,000 ng/mL for tizanidine, and 20–10,000 ng/mL for diclofenac. No chromatographic interference from tablet excipients was found. In order to check the selectivity of the proposed method, degradation studies were carried out using hydrolysis (acid, basic, and neutral), thermolysis, and oxidation. The developed method, after being validated in terms of precision, robustness, recovery, LOD, and LOQ, was successively applied to the analysis of pharmaceutical formulations and human serum.

Simultaneous determination of piperacillin and tazobactam in pharmaceutical formulations by RP-HPLC method

2013 ◽  
Vol 7 (1) ◽  
pp. 127-131 ◽  
Author(s):  
P. Rama Krishna Veni ◽  
N. Sharmila ◽  
K.J.P. Narayana ◽  
B. Hari Babu ◽  
P.V.V. Satyanarayana
Keyword(s):  
Simultaneous Determination ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Rp Hplc
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