Validated Method for the Simultaneous Determination of Gemifloxacin and H2-receptor Antagonists in Bulk, Pharmaceutical Formulations and Human Serum by RP-HPLC; In-vitro Applications

2011 ◽  
Vol 58 (5) ◽  
pp. 629-636 ◽  
Author(s):  
Najma Sultana ◽  
M. Saeed Arayne ◽  
Sana Shamim ◽  
Asia Naz
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Pharmaceutical Formulations ◽  
Receptor Antagonists ◽  
H2 Receptor Antagonists ◽  
H2 Receptor ◽  
Rp Hplc

Determination of histamine H2 receptor antagonists in pharmaceutical formulations by CE-MS

2014 ◽  
Vol 6 (6) ◽  
pp. 1714-1719 ◽  
Author(s):  
Juan José Berzas Nevado ◽  
Gregorio Castañeda Peñalvo ◽  
Rosa Ma Rodríguez Dorado ◽  
Virginia Rodríguez Robledo
Keyword(s):  
Pharmaceutical Formulations ◽  
Receptor Antagonists ◽  
H2 Receptor Antagonists ◽  
Histamine H2 Receptor ◽  
H2 Receptor ◽  
Qualitative And Quantitative ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Esi Mass Spectrometry

A simple, fast and new method for the qualitative and quantitative determination of histamine H2 receptor antagonists by using capillary zone electrophoresis (CZE) coupled to electrospray ionization (ESI) mass spectrometry (MS) has been developed.

Simultaneous Determination of Chloroquine and Pyrimethamine with Cetirizine in an Active Form and Human Serum by RP-HPLC

2021 ◽  
Author(s):  
Hina Shamshad ◽  
Ali Sayqal ◽  
Jahan Zeb ◽  
Agha Zeeshan Mirza
Keyword(s):  
Human Serum ◽  
Simultaneous Determination ◽  
Active Form ◽  
Internal Standard ◽  
Hplc Method ◽  
Serum Samples ◽  
Rp Hplc ◽  
Cetirizine Hydrochloride ◽  
Bulk Drug

Abstract A simple, accurate and precise RP-HPLC method was developed for the simultaneous determination of chloroquine, pyrimethamine and cetirizine hydrochloride concentrations in bulk drug and human serum. The assay was performed using a mobile phase of methanol: water (70:30) at pH of 2.8 ± 0.05 on the Purospher C-18 column with UV detection at 230 nm and rosuvastatin used as an internal standard. The retention times observed for chloroquine, pyrimethamine and cetirizine hydrochloride were 3.5, 2.5 and 5.5 minutes, respectively. The method was found to be specific for the assayed drugs showing a linear response in the concentration range of 1–100 μg mL−1 with coefficients of determination values of (r = 0.999). The method was developed and validated according to ICH guidelines. The method was used to monitor the serum samples and was found to be sensitive for therapeutic purposes, showing the potential to be a useful tool for routine analysis in laboratories.

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