scholarly journals Determination of <i>α</i>-1,3-Linked Mannose Residue in the Cell Wall Mannan of <i>Candida tropicalis</i> NBRC 1400 Strain

2020 ◽  
Vol 10 (01) ◽  
pp. 14-26
Author(s):  
Takuya Kuraoka ◽  
Takayoshi Yamada ◽  
Akito Ishiyama ◽  
Hiroko Oyamada ◽  
Yukiko Ogawa ◽  
...  
Keyword(s):  
Cell Wall ◽  
Candida Tropicalis ◽  
Mannose Residue ◽  
Cell Wall Mannan

scholarly journals Structural characteristics of plant cell wall elucidated by solution-state 2D NMR spectroscopy with an optimized procedure

2020 ◽  
Vol 9 (1) ◽  
pp. 650-663
Author(s):  
Wanwan Wang ◽  
Jibao Cai ◽  
Zhenyu Xu ◽  
Yi Zhang ◽  
Fanchao Niu ◽  
...  
Keyword(s):  
Cell Wall ◽  
Quantum Coherence ◽  
Nmr Spectra ◽  
Plant Cell Wall ◽  
Milling Time ◽  
Structural Characteristics ◽  
2D Nmr ◽  
2D Nmr Spectroscopy ◽  
Complete Representation

AbstractA method was developed for rapid qualitative determination of lignocellulose in the tobacco cell wall by utilizing 2D heteronuclear single quantum coherence NMR spectra (2D HSQC NMR). Traditional methods for analyzing the structure of lignocellulose involve many steps of separation and extraction, which is labor-intensive. In this work, the whole cell wall was milled and dissolved in deuterium solvent. The solvent dimethylsulfoxide (DMSO-d6) containing hexamethylphosphoramide (HMPA-d18) enhanced swelling of the sample and gave high-resolution spectra. The tobacco samples are ball milled at different ball milling times, and the state of the particles is observed through an electron microscope, and then the probability of the particles being less than 5 µm is counted. Through the comparison of the abundance and integration of the peak signals in the spectra under different transmittances, it was determined that when the milling time was 6 h, the quality of the NMR spectra was the best. The optimum conditions of characterizing tobacco structure were DMSO-d6/HMPA-d18 solution and 6 h milling time. Under these conditions, complete representation of the structure of lignocellulose and simplified process could be achieved.

Influence of oxidative and osmotic stresses on the structure of the cell wall mannan of Candida albicans serotype A

2009 ◽  
Vol 344 (16) ◽  
pp. 2195-2200 ◽  
Author(s):  
Takashi Koyama ◽  
Mayumi Makita ◽  
Nobuyuki Shibata ◽  
Yoshio Okawa
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Serotype A ◽  
Cell Wall Mannan

scholarly journals Localisation and characterisation of cell wall mannan polysaccharides in Arabidopsis thaliana

Planta ◽  
2003 ◽  
Vol 218 (1) ◽  
pp. 27-36 ◽  
Author(s):  
Michael G. Handford ◽  
Timothy C. Baldwin ◽  
Florence Goubet ◽  
Tracy A. Prime ◽  
Joanne Miles ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Cell Wall Mannan

scholarly journals Biosynthesis of cell wall mannan in the conidium and the mycelium ofAspergillusfumigatus

2016 ◽  
Vol 18 (12) ◽  
pp. 1881-1891 ◽  
Author(s):  
Christine Henry ◽  
Thierry Fontaine ◽  
Christoph Heddergott ◽  
Pauline Robinet ◽  
Vishukumar Aimanianda ◽  
...  
Keyword(s):  
Cell Wall ◽  
Cell Wall Mannan

Pharmacognostic Studies of the Stem Bark of Enantia chlorantha Oliver (Annonaceae)

2015 ◽  
Vol 19 ◽  
pp. 122-125
Author(s):  
TL Ohemu ◽  
A Agunu ◽  
DG Dafam ◽  
PN Olotu
Keyword(s):  
Cell Wall ◽  
Calcium Oxalate ◽  
Stem Bark ◽  
Standard Methods ◽  
Antiulcer Agents ◽  
Physiochemical Parameters ◽  
Microscopic Studies ◽  
Wall Materials ◽  
Cell Wall Materials

Enantia chlorantha Oliver (Annonaceae) is commonly known as African yellow wood used as hepatoprotective, antiviral, antimalarial, antibacterial and antiulcer agents. The study was aimed to investigate the pharmacognostic and physiochemical parameters of E. chlorantha stem bark. The macroscopy, microscopy and chemomicroscopy of E. chlorantha were carried out using standard methods. Cell wall materials, cell inclusions and other diagnostic characters, which can aid in the easy and proper identification of the plant, were identified. The microscopic studies revealed the presence of sclereids, fibres, medullary ray, and calcium oxalate prisms. The physiochemical evaluation of was done, in order to ascertain quality and purity. This study provides additional useful information needed for determination of its identity and quality that can be added as enrichment of the pharmacopoeia of the plant.Keywords: Pharmacognostic, Stem Bark, Enantia chlorantha

scholarly journals Properties of Arabinogalactan Proteins (AGPs) in Apple (Malus × Domestica) Fruit at Different Stages of Ripening

Biology ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 225
Author(s):  
Agata Leszczuk ◽  
Justyna Cybulska ◽  
Tomasz Skrzypek ◽  
Artur Zdunek
Keyword(s):  
Cell Wall ◽  
Malus Domestica ◽  
Arabinogalactan Proteins ◽  
Ex Situ ◽  
Morphological Properties ◽  
Mature Stage ◽  
Fruit Storage ◽  
Cell Wall Assembly ◽  
Wall Assembly

Arabinogalactan proteins (AGPs) are constituents of the cell wall–plasma membrane continuum in fruit tissue. The aim of the study was to characterise AGPs contained in fruit by determination of their chemical structure and morphological properties. The results were obtained from in and ex situ investigations and a comparative analysis of AGPs present in Malus × domestica fruit at different stages of ripening from green fruit through the mature stage to over-ripening during fruit storage. The HPLC and colorimetric methods were used for analyses of the composition of monosaccharides and proteins in AGPs extracted from fruit. We have found that AGPs from fruit mainly consists of carbohydrate chains composed predominantly of arabinose, galactose, glucose, galacturonic acid, and xylose. The protein moiety accounts for 3.15–4.58%, which depends on the various phases of ripening. Taken together, our results show that the structural and morphological properties of AGPs and calcium concentration in AGPs are related to the progress of ripening, which is correlated with proper fruit cell wall assembly. In line with the existing knowledge, our data confirmed the typical carbohydrate composition of AGPs and may be the basis for studies regarding their presumed properties of binding calcium ions.

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