scholarly journals PUB16 gene expression under abiotic stress and their putative role as an ARM repeat protein in <i>Arabidopsis thaliana</i> self-pollination pathway

2012 ◽  
Vol 03 (05) ◽  
pp. 609-619 ◽  
Author(s):  
María Gabriela Acosta ◽  
Miguel Ángel Ahumada ◽  
Sergio Luis Lassaga ◽  
Víctor Hugo Casco
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Repeat Protein ◽  
Putative Role ◽  
Self Pollination

scholarly journals Profiling the Abiotic Stress Responsive microRNA Landscape of Arabidopsis thaliana

Plants ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 58 ◽  
Author(s):  
Joseph Pegler ◽  
Jackson Oultram ◽  
Christopher Grof ◽  
Andrew Eamens
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Mirna Target ◽  
Target Gene ◽  
Negative Impact ◽  
Mirna Target Gene ◽  
Target Gene Expression ◽  
Response Phenotype

It is well established among interdisciplinary researchers that there is an urgent need to address the negative impacts that accompany climate change. One such negative impact is the increased prevalence of unfavorable environmental conditions that significantly contribute to reduced agricultural yield. Plant microRNAs (miRNAs) are key gene expression regulators that control development, defense against invading pathogens and adaptation to abiotic stress. Arabidopsis thaliana (Arabidopsis) can be readily molecularly manipulated, therefore offering an excellent experimental system to alter the profile of abiotic stress responsive miRNA/target gene expression modules to determine whether such modification enables Arabidopsis to express an altered abiotic stress response phenotype. Towards this goal, high throughput sequencing was used to profile the miRNA landscape of Arabidopsis whole seedlings exposed to heat, drought and salt stress, and identified 121, 123 and 118 miRNAs with a greater than 2-fold altered abundance, respectively. Quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) was next employed to experimentally validate miRNA abundance fold changes, and to document reciprocal expression trends for the target genes of miRNAs determined abiotic stress responsive. RT-qPCR also demonstrated that each miRNA/target gene expression module determined to be abiotic stress responsive in Arabidopsis whole seedlings was reflective of altered miRNA/target gene abundance in Arabidopsis root and shoot tissues post salt stress exposure. Taken together, the data presented here offers an excellent starting platform to identify the miRNA/target gene expression modules for future molecular manipulation to generate plant lines that display an altered response phenotype to abiotic stress.

scholarly journals Genome Identification and CtCP1 Gene expression of Cysteine Protease in Carthamus Tinctorius under Abiotic Stress

2021 ◽  
Author(s):  
Yingqi Hong ◽  
Lv Yanxi ◽  
Zhang Jianyi ◽  
Nguyen Quoc Viet Hoang ◽  
Li Youbao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Low Temperature ◽  
Gene Structure ◽  
Cysteine Protease ◽  
Carthamus Tinctorius ◽  
Whole Genome ◽  
Qrt Pcr ◽  
Transgenic Lines

Abstract BackgroundCysteine protease (CP) plays an important role in plant senescence. In this study, the whole genome evolution information of CPs was analyzed by using safflower, and the function of CtCP1 under l abiotic stress was analyzed.Results25 CPs members were identified in the safflower genome and divided into 9 subfamilies. Gene structure analysis showed that the possible evolutionary conservatism and functional similarity of the same family members. qRT-PCR at different florescence showed that the expression of CPs gene was the highest in the decline period, and CtCP1 gene changed significantly under abiotic stress. We cloned the qRT-PCR of CtCP1, at different florescence and stress, which showed that the expression of CtCP1 was the highest in the decline stage and low temperature. In order to study the function of CtCP1 gene, we obtained the overexpression CtCP1 line (OE) and the inhibition CtCP1 expression line (Anti) in Arabidopsis thaliana. The results of transgenic lines under low temperature stress showed that inhibition of CtCP1 expression enhanced the resistance of Carthamus tinctorius to low temperature, and overexpression of CtCP1 weakened the resistance of Carthamus tinctorius to low temperature.ConclusionWe have identified the cysteine protease genome of safflower and CtCP1 gene expression under abiotic stress

scholarly journals The Plant-Growth-Promoting Rhizobacterium Paenibacillus polymyxa Induces Changes in Arabidopsis thaliana Gene Expression: A Possible Connection Between Biotic and Abiotic Stress Responses

1999 ◽  
Vol 12 (11) ◽  
pp. 951-959 ◽  
Author(s):  
Salme Timmusk ◽  
E. Gerhart H. Wagner
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Plant Growth ◽  
Stress Responses ◽  
Biotic Stress ◽  
Paenibacillus Polymyxa ◽  
Mrna Levels ◽  
Plant Growth Promoting ◽  
Growth Promoting

This paper addresses changes in plant gene expression induced by inoculation with plant-growth-promoting rhizobacteria (PGPR). A gnotobiotic system was established with Arabidopsis thaliana as model plant, and isolates of Paenibacillus polymyxa as PGPR. Subsequent challenge by either the pathogen Erwinia carotovora (biotic stress) or induction of drought (abiotic stress) indicated that inoculated plants were more resistant than control plants. With RNA differential display on parallel RNA preparations from P. polymyxa- treated or untreated plants, changes in gene expression were investigated. From a small number of candidate sequences obtained by this approach, one mRNA segment showed a strong inoculation-dependent increase in abundance. The corresponding gene was identified as ERD15, previously identified to be drought stress responsive. Quantification of mRNA levels of several stress-responsive genes indicated that P. polymyxa induced mild biotic stress. This suggests that genes and/or gene classes associated with plant defenses against abiotic and biotic stress may be co-regulated. Implications of the effects of PGPR on the induction of plant defense pathways are discussed.

scholarly journals Natural Variation in Abiotic Stress Responsive Gene Expression and Local Adaptation to Climate in Arabidopsis thaliana

2014 ◽  
Vol 31 (9) ◽  
pp. 2283-2296 ◽  
Author(s):  
Jesse R. Lasky ◽  
David L. Des Marais ◽  
David B. Lowry ◽  
Inna Povolotskaya ◽  
John K. McKay ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Local Adaptation ◽  
Natural Variation ◽  
Responsive Gene
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