scholarly journals The Association Among Gene Expression Responses to Nine Abiotic Stress Treatments in Arabidopsis thaliana

Genetics ◽  
2006 ◽  
Vol 174 (4) ◽  
pp. 1811-1824 ◽  
Author(s):  
William R. Swindell
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Abiotic Stress

scholarly journals Profiling the Abiotic Stress Responsive microRNA Landscape of Arabidopsis thaliana

Plants ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 58 ◽  
Author(s):  
Joseph Pegler ◽  
Jackson Oultram ◽  
Christopher Grof ◽  
Andrew Eamens
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Salt Stress ◽  
Abiotic Stress ◽  
Mirna Target ◽  
Target Gene ◽  
Negative Impact ◽  
Mirna Target Gene ◽  
Target Gene Expression ◽  
Response Phenotype

It is well established among interdisciplinary researchers that there is an urgent need to address the negative impacts that accompany climate change. One such negative impact is the increased prevalence of unfavorable environmental conditions that significantly contribute to reduced agricultural yield. Plant microRNAs (miRNAs) are key gene expression regulators that control development, defense against invading pathogens and adaptation to abiotic stress. Arabidopsis thaliana (Arabidopsis) can be readily molecularly manipulated, therefore offering an excellent experimental system to alter the profile of abiotic stress responsive miRNA/target gene expression modules to determine whether such modification enables Arabidopsis to express an altered abiotic stress response phenotype. Towards this goal, high throughput sequencing was used to profile the miRNA landscape of Arabidopsis whole seedlings exposed to heat, drought and salt stress, and identified 121, 123 and 118 miRNAs with a greater than 2-fold altered abundance, respectively. Quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) was next employed to experimentally validate miRNA abundance fold changes, and to document reciprocal expression trends for the target genes of miRNAs determined abiotic stress responsive. RT-qPCR also demonstrated that each miRNA/target gene expression module determined to be abiotic stress responsive in Arabidopsis whole seedlings was reflective of altered miRNA/target gene abundance in Arabidopsis root and shoot tissues post salt stress exposure. Taken together, the data presented here offers an excellent starting platform to identify the miRNA/target gene expression modules for future molecular manipulation to generate plant lines that display an altered response phenotype to abiotic stress.

scholarly journals Genome Identification and CtCP1 Gene expression of Cysteine Protease in Carthamus Tinctorius under Abiotic Stress

2021 ◽  
Author(s):  
Yingqi Hong ◽  
Lv Yanxi ◽  
Zhang Jianyi ◽  
Nguyen Quoc Viet Hoang ◽  
Li Youbao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Low Temperature ◽  
Gene Structure ◽  
Cysteine Protease ◽  
Carthamus Tinctorius ◽  
Whole Genome ◽  
Qrt Pcr ◽  
Transgenic Lines

Abstract BackgroundCysteine protease (CP) plays an important role in plant senescence. In this study, the whole genome evolution information of CPs was analyzed by using safflower, and the function of CtCP1 under l abiotic stress was analyzed.Results25 CPs members were identified in the safflower genome and divided into 9 subfamilies. Gene structure analysis showed that the possible evolutionary conservatism and functional similarity of the same family members. qRT-PCR at different florescence showed that the expression of CPs gene was the highest in the decline period, and CtCP1 gene changed significantly under abiotic stress. We cloned the qRT-PCR of CtCP1, at different florescence and stress, which showed that the expression of CtCP1 was the highest in the decline stage and low temperature. In order to study the function of CtCP1 gene, we obtained the overexpression CtCP1 line (OE) and the inhibition CtCP1 expression line (Anti) in Arabidopsis thaliana. The results of transgenic lines under low temperature stress showed that inhibition of CtCP1 expression enhanced the resistance of Carthamus tinctorius to low temperature, and overexpression of CtCP1 weakened the resistance of Carthamus tinctorius to low temperature.ConclusionWe have identified the cysteine protease genome of safflower and CtCP1 gene expression under abiotic stress

scholarly journals The Plant-Growth-Promoting Rhizobacterium Paenibacillus polymyxa Induces Changes in Arabidopsis thaliana Gene Expression: A Possible Connection Between Biotic and Abiotic Stress Responses

1999 ◽  
Vol 12 (11) ◽  
pp. 951-959 ◽  
Author(s):  
Salme Timmusk ◽  
E. Gerhart H. Wagner
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Plant Growth ◽  
Stress Responses ◽  
Biotic Stress ◽  
Paenibacillus Polymyxa ◽  
Mrna Levels ◽  
Plant Growth Promoting ◽  
Growth Promoting

This paper addresses changes in plant gene expression induced by inoculation with plant-growth-promoting rhizobacteria (PGPR). A gnotobiotic system was established with Arabidopsis thaliana as model plant, and isolates of Paenibacillus polymyxa as PGPR. Subsequent challenge by either the pathogen Erwinia carotovora (biotic stress) or induction of drought (abiotic stress) indicated that inoculated plants were more resistant than control plants. With RNA differential display on parallel RNA preparations from P. polymyxa- treated or untreated plants, changes in gene expression were investigated. From a small number of candidate sequences obtained by this approach, one mRNA segment showed a strong inoculation-dependent increase in abundance. The corresponding gene was identified as ERD15, previously identified to be drought stress responsive. Quantification of mRNA levels of several stress-responsive genes indicated that P. polymyxa induced mild biotic stress. This suggests that genes and/or gene classes associated with plant defenses against abiotic and biotic stress may be co-regulated. Implications of the effects of PGPR on the induction of plant defense pathways are discussed.

scholarly journals Natural Variation in Abiotic Stress Responsive Gene Expression and Local Adaptation to Climate in Arabidopsis thaliana

2014 ◽  
Vol 31 (9) ◽  
pp. 2283-2296 ◽  
Author(s):  
Jesse R. Lasky ◽  
David L. Des Marais ◽  
David B. Lowry ◽  
Inna Povolotskaya ◽  
John K. McKay ◽  
...  
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Abiotic Stress ◽  
Local Adaptation ◽  
Natural Variation ◽  
Responsive Gene

scholarly journals Alternative splicing landscapes in Arabidopsis thaliana across tissues and stress conditions highlight major functional differences with animals

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Guiomar Martín ◽  
Yamile Márquez ◽  
Federica Mantica ◽  
Paula Duque ◽  
Manuel Irimia
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Alternative Splicing ◽  
Stress Responses ◽  
Target Genes ◽  
Intron Retention ◽  
Single Gene ◽  
Exon Skipping ◽  
Environmental Response ◽  
Biotic Stresses

Abstract Background Alternative splicing (AS) is a widespread regulatory mechanism in multicellular organisms. Numerous transcriptomic and single-gene studies in plants have investigated AS in response to specific conditions, especially environmental stress, unveiling substantial amounts of intron retention that modulate gene expression. However, a comprehensive study contrasting stress-response and tissue-specific AS patterns and directly comparing them with those of animal models is still missing. Results We generate a massive resource for Arabidopsis thaliana, PastDB, comprising AS and gene expression quantifications across tissues, development and environmental conditions, including abiotic and biotic stresses. Harmonized analysis of these datasets reveals that A. thaliana shows high levels of AS, similar to fruitflies, and that, compared to animals, disproportionately uses AS for stress responses. We identify core sets of genes regulated specifically by either AS or transcription upon stresses or among tissues, a regulatory specialization that is tightly mirrored by the genomic features of these genes. Unexpectedly, non-intron retention events, including exon skipping, are overrepresented across regulated AS sets in A. thaliana, being also largely involved in modulating gene expression through NMD and uORF inclusion. Conclusions Non-intron retention events have likely been functionally underrated in plants. AS constitutes a distinct regulatory layer controlling gene expression upon internal and external stimuli whose target genes and master regulators are hardwired at the genomic level to specifically undergo post-transcriptional regulation. Given the higher relevance of AS in the response to different stresses when compared to animals, this molecular hardwiring is likely required for a proper environmental response in A. thaliana.

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