scholarly journals Optimization of the bioconversion of glycerol to ethanol using <i>Escherichia coli</i> by implementing a bi-level programming framework for proposing gene transcription control strategies based on genetic algorithms

2012 ◽  
Vol 03 (04) ◽  
pp. 336-343 ◽  
Author(s):  
Carol Milena Barreto-Rodriguez ◽  
Jessica Paola Ramirez-Angulo ◽  
Jorge Mario Gomez-Ramirez ◽  
Luke Achenie ◽  
Andres Fernando Gonzalez-Barrios
Keyword(s):  
Escherichia Coli ◽  
Genetic Algorithms ◽  
Gene Transcription ◽  
Control Strategies ◽  
Transcription Control ◽  
Programming Framework

scholarly journals Expression Characteristics of the Transfer-RelatedkilB Gene Product of Streptomyces Plasmid pIJ101: Implications for the Plasmid Spread Function

2001 ◽  
Vol 183 (4) ◽  
pp. 1339-1345 ◽  
Author(s):  
Gregg S. Pettis ◽  
Naomi Ward ◽  
Kevin L. Schully
Keyword(s):  
Escherichia Coli ◽  
Fusion Protein ◽  
Secondary Metabolism ◽  
Gene Product ◽  
Gene Transcription ◽  
Cellular Differentiation ◽  
Developmental Stages ◽  
Agar Medium ◽  
Spread Function ◽  
Aerial Hyphae

ABSTRACT Intermycelial transfer of Streptomyces plasmid pIJ101 occurs prior to cellular differentiation and is mediated by plasmid functions that are also required for production of zones of growth-inhibited recipient cells (i.e., pocks) that develop around individual donors during mating on agar medium. Several other pIJ101 functions, including that of the kilB gene, whose unregulated expression on pIJ101 is lethal, are required for normal pock size and so have been postulated to mediate intramycelial spread of the plasmid throughout recipient cells. Using antibodies raised against a KilB fusion protein expressed in Escherichia coli, native KilB protein was detected throughout development of pIJ101-containing Streptomyces lividans cells, with the concentration of KilB increasing dramatically and reaching a maximum during the final stages (i.e., sporulation and secondary metabolism) of cellular differentiation. Insertion of the kilB gene of pIJ101 into the S. lividans chromosome in cells lacking the pIJ101 KorB protein, which normally represses kilB gene transcription, resulted in elevated but still temporally increasing amounts of KilB. The increased expression or accumulation of the KilB spread protein throughout cellular differentiation of S. lividans, which leads to maximum KilB concentrations during developmental stages that occur far later than when intermycelial transfer of pIJ101 is mediated, supports the existence of a subsequent intramycelial component to the pIJ101 spread function. The results also suggest that intramycelial spread of pIJ101 molecules within the recipient extends beyond intercompartmental movements within the substrate mycelia and includes undetermined steps within the spore-yielding aerial hyphae as well.

Multi-Objective Optimization Design of Control Devices to Suppress Tall Buildings Vibrations against Earthquake Excitations Using Fuzzy Logic and Genetic Algorithms

2015 ◽  
pp. 787-817
Author(s):  
Saeid Pourzeynali ◽  
Shide Salimi
Keyword(s):  
Genetic Algorithms ◽  
Fuzzy Logic ◽  
Optimization Design ◽  
Base Isolation ◽  
Control Strategies ◽  
Optimal Solution ◽  
Base Shear ◽  
Viscous Dampers ◽  
Nsga Ii ◽  
Isolation Systems

The main objective of this chapter is to find the optimal values of the parameters of the base isolation systems and that of the semi-active viscous dampers using genetic algorithms (GAs) and fuzzy logic in order to simultaneously minimize the buildings' selected responses such as displacement of the top story, base shear, and so on. In this study, performance of base isolation systems, and semi-active viscous dampers are studied separately as different vibration control strategies. In order to simultaneously minimize the objective functions, a fast and elitist non-dominated sorting genetic algorithm (NSGA-II) approach is used to find a set of Pareto-optimal solution. To study the performance of semi-active viscous dampers, the torsional effects exist in the building due to irregularities, and unsymmetrical placement of the dampers is taken into account through 3D modeling of the building.

scholarly journals Molecular analysis of porin gene transcription in heterogenotypic multidrug-resistant Escherichia coli isolates from scouring calves

2010 ◽  
Vol 65 (9) ◽  
pp. 1926-1935 ◽  
Author(s):  
Heather M. Vinson ◽  
Ablesh Gautam ◽  
Susan Olet ◽  
Penelope S. Gibbs ◽  
Robert Barigye
Keyword(s):  
Escherichia Coli ◽  
Molecular Analysis ◽  
Gene Transcription ◽  
Multidrug Resistant

scholarly journals Risk Factors for Infection or Colonization with CTX-M Extended-Spectrum-β-Lactamase-Positive Escherichia coli

2012 ◽  
Vol 56 (11) ◽  
pp. 5575-5580 ◽  
Author(s):  
Jennifer H. Han ◽  
Kei Kasahara ◽  
Paul H. Edelstein ◽  
Warren B. Bilker ◽  
Ebbing Lautenbach
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Control Strategies ◽  
Content Type ◽  
E Coli ◽  
Extended Spectrum ◽  
Increased Risk ◽  
Urinary Culture ◽  
Multivariable Analyses ◽  
And Control

ABSTRACTThere has been a significant increase in the prevalence ofEnterobacteriaceaethat produce CTX-M-type extended-spectrum β-lactamases. The objective of this study was to evaluate risk factors for infection or colonization with CTX-M-positiveEscherichia coli. A case-control study was conducted within a university system from 1 January 2007 to 31 December 2008. All patients with clinical cultures withE. colidemonstrating resistance to extended-spectrum cephalosporins were included. Case patients were designated as those with cultures positive for CTX-M-positiveE. coli, and control patients were designated as those with non-CTX-M-producingE. coli. Multivariable logistic regression analyses were performed to evaluate risk factors for CTX-M-positive isolates. A total of 83 (56.8%) of a total of 146 patients had cultures with CTX-M-positiveE. coli. On multivariable analyses, there was a significant association between infection or colonization with CTX-M-type β-lactamase-positiveE. coliand receipt of piperacillin-tazobactam in the 30 days prior to the culture date (odds ratio [OR], 7.36; 95% confidence interval [CI], 1.61 to 33.8;P= 0.01) and a urinary culture source (OR, 0.36; 95% CI, 0.17 to 0.77;P= 0.008). The rates of resistance to fluoroquinolones were significantly higher in isolates from case patients than in isolates from control patients (90.4 and 50.8%, respectively;P< 0.001). We found that nonurinary sources of clinical cultures and the recent use of piperacillin-tazobactam conferred an increased risk of colonization or infection with CTX-M-positiveE. coli. Future studies will need to focus on outcomes associated with infections due to CTX-M-positiveE. coli, as well as infection control strategies to limit the spread of these increasingly common organisms.

scholarly journals Repression of btuB gene transcription in Escherichia coli by the GadX protein

2011 ◽  
Vol 11 (1) ◽  
pp. 33 ◽  
Author(s):  
Guang-Sheng Lei ◽  
Wan-Jr Syu ◽  
Po-Huang Liang ◽  
Kin-Fu Chak ◽  
Wensi S Hu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gene Transcription
Sign in / Sign up

Export Citation Format

Share Document