Auxins Stimulated the Axillary Branching of Atriplex nummularia L. In Vitro

1997 ◽  
Vol 8 (1) ◽  
pp. 61-65
Author(s):  
ABDELRAHMAN AL-WASEL
Keyword(s):  
Atriplex Nummularia ◽  
Axillary Branching

In vitro propagation of an adult tree Wrightia tomentosa through enhanced axillary branching

Plant Science ◽  
1994 ◽  
Vol 103 (1) ◽  
pp. 67-72 ◽  
Author(s):  
S.D. Purohit ◽  
G. Kukda ◽  
P. Sharma ◽  
K. Tak
Keyword(s):  
In Vitro Propagation ◽  
Axillary Branching ◽  
Adult Tree

scholarly journals Effect of cytokinin and cultivar on shoot formation of Gerbera jamesonii in vitro.

1982 ◽  
Vol 30 (4) ◽  
pp. 341-346 ◽  
Author(s):  
R.L.M. Pierik ◽  
H.H.M. Steegmans ◽  
J.A.M. Verhaegh ◽  
A.N. Wouters
Keyword(s):  
Clonal Propagation ◽  
Callus Formation ◽  
Shoot Formation ◽  
Optimum Level ◽  
Gerbera Jamesonii ◽  
Axillary Shoots ◽  
Cytokinin Level ◽  
Leaf Weight ◽  
Axillary Branching

The success of gerbera clonal propagation in vitro, using either capitulum explants or subcultured shoots in trials with up to 28 cvs, depended both on the cv. and on the cytokinin level in the medium. With capitulum explants, shoot formation was very low for some cvs regardless of the level of BA (5, 10 or 20 mg/l) whereas other cvs had individual optimum BA levels. Axillary branching of subcultured shoots differed between cvs and between levels of kinetin (1, 5 or 10 mg/l) in the medium. The optimum level for the highest quality shoots (highest leaf weight/shoot, no leaf malformation and no callus formation) was not always the same as the level producing the highest number of axillary shoots. Results are tabulated for each cv. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals Micropropagation of Mountain Mulberry (Morus bombycis Koidz.) ‘Kenmochi’ on Cytokinin-Free Medium

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1533
Author(s):  
Wojciech Litwińczuk ◽  
Beata Jacek
Keyword(s):  
Good Alternative ◽  
In Vitro Cultures ◽  
Axillary Shoot ◽  
Free Medium ◽  
Single Node ◽  
Potted Plants ◽  
Adult Trees ◽  
Axillary Branching

The aim of the study was to compare two methods of micropropagation of mulberry: single-node culture (“SNC”), and axillary-branching (“AxB”). The experiments were carried out on in vitro cultures for 6 successive passages. The “AxB” cultures were propagated on modified MS medium (+ 25% Ca2+ and Mg2+), supplemented with WPM vitamins, sucrose (30 g L−1), and BA (1.5 mg l-1). The “SNC” cultures were grown on cytokinin-free 1/2 MS (macro- and micronutrients) medium supplemented with WPM vitamins, IBA (0.05 mg l-1), and sucrose (15 g l-1). Both media (pH 5.8) were solidified with agar (7.0 g l-1). Initiation of in vitro cultures from explants taken from adult trees and young, potted plants was feasible on both media. Cultures were established from about 1 cm long nodal explants. Generally “SNC” cultures formed one well rooted, significantly longer axillary shoot with bigger leaves than “AxB” cultures, which developed significantly more shoots and big callus at the explant base. All shoots collected from “SNC” and “AxB” cultures rooted in vivo in peat mixture and developed into similar plantlets. The single-node method based on application of cytokinin-free medium is a good alternative for the axillary-branching method for micropropagation of mountain mulberry (Morus bombycis) ‘Kenmochi”’.

scholarly journals In vitro clonal propagation of bael (Aegle marmelos Corr.) CV. CISH-B1 through enhanced axillary branching

2008 ◽  
Vol 14 (4) ◽  
pp. 337-346 ◽  
Author(s):  
Rajesh Pati ◽  
Ramesh Chandra ◽  
Ugam Kumari Chauhan ◽  
Maneesh Mishra ◽  
Navin Srivastava
Keyword(s):  
Clonal Propagation ◽  
Aegle Marmelos ◽  
Axillary Branching ◽  
In Vitro Clonal Propagation

Ultrastructural Investigations of the Contractile Filaments of Amoebae

1974 ◽  
Vol 32 ◽  
pp. 188-189
Author(s):  
P.L. Moore
Keyword(s):  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Amoeboid Movement ◽  
Different Types ◽  
Chemical Conditions ◽  
Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

1974 ◽  
Vol 32 ◽  
pp. 132-133
Author(s):  
John J. Wolosewick ◽  
John H. D. Bryan
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Nuclear Ring ◽  
Rough Er ◽  
Distal Direction ◽  
In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

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