Multiplication of the endangered Indian pitcher plant (Nepenthes khasiana) through enhanced axillary branching in vitro

1994 ◽  
Vol 38 (1) ◽  
pp. 69-71 ◽  
Author(s):  
P. G. Latha ◽  
S. Seeni
Keyword(s):  
Pitcher Plant ◽  
Axillary Branching ◽  
Nepenthes Khasiana

scholarly journals In vitro Multiplication of the Pitcher Plant Sarracenia Purpurea

2018 ◽  
Vol 75 (2) ◽  
pp. 134
Author(s):  
Ileana MICLEA ◽  
Rita BERNAT
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Multiplication ◽  
Time Frame ◽  
Naphthaleneacetic Acid ◽  
Sarracenia Purpurea ◽  
Pitcher Plant ◽  
Frame Number

The aim of the current research was to find the best plant growth regulators for the multiplication of Sarracenia purpurea. Murashige and Skoog medium (MS) was prepared with macronutrients and micronutrients at 1/3 strength, full strength vitamins, supplemented with 30 g/l sucrose and 5 g/l phytagel and autoclaved. After cooling 0.5 mg\l α-naphthaleneacetic acid (NAA), 5 mg\l 6-benzyladenine (BA) or 0.5 mg\l NAA + 3 mg\l BA were added. Young S. purpurea plants were selected and transferred to media with or without plant growth regulators and cultured for 12 weeks. At the end of this time frame number of roots, root length (cm) and number of shoots were evaluated and differences were analysed by the analysis of variance and interpreted using the Tuckey test. The largest number of roots grew in medium supplemented with 0.5 mg\l NAA but the the absence of plant growth regulators increased their length. The best conditions for shoot multiplication were provided by supplementing 1/3MS with 5 mg\l BA.

In vitro propagation of an adult tree Wrightia tomentosa through enhanced axillary branching

Plant Science ◽  
1994 ◽  
Vol 103 (1) ◽  
pp. 67-72 ◽  
Author(s):  
S.D. Purohit ◽  
G. Kukda ◽  
P. Sharma ◽  
K. Tak
Keyword(s):  
In Vitro Propagation ◽  
Axillary Branching ◽  
Adult Tree

Occurrence and growth characteristics ofEscherichia coliand enterococci within the accumulated fluid of the northern pitcher plant (Sarracenia purpureaL.)

2005 ◽  
Vol 51 (12) ◽  
pp. 1027-1037 ◽  
Author(s):  
Richard L Whitman ◽  
Stacey E Byers ◽  
Dawn A Shively ◽  
Donna M Ferguson ◽  
Muruleedhara Byappanahalli
Keyword(s):  
Escherichia Coli ◽  
Growth Potential ◽  
Indicator Bacteria ◽  
Natural Conditions ◽  
Pitcher Plant ◽  
Fecal Indicator ◽  
E Coli ◽  
Fluid Concentration ◽  
The Individual

Sarracenia purpurea L., a carnivorous bog plant (also known as the pitcher plant), represents an excellent model of a well-defined, self-contained ecosystem; the individual pitchers of the plant serve as a microhabitat for a variety of micro- and macro-organisms. Previously, fecal indicator bacteria (Escherichia coli and enterococci) were shown as incidental contaminants in pitcher fluid; however, whether their occurrence in pitcher fluid is incidental or common has not been established. The purpose of this study was to investigate the occurrence, distribution, and growth potential of E. coli and enterococci in pitcher plant fluid from a protected bog in northwest Indiana. Escherichia coli and enterococci were recovered in pitcher fluids (n = 43 plants), with mean densities (log CFU mL–1) of 1.28 ± 0.23 and 1.97 ± 0.27, respectively. In vitro experiments showed that E. coli growth in fluid not containing insects or indigenous organisms was directly proportional to the fluid concentration (growth was 10-fold in 24 h in 100% fluid); however, in the presence of other indigenous organisms, E. coli and enterococci were only sustained for 5 days at 26 °C. Pulsed-field gel electrophoresis (PFGE) analysis showed that the plant Enterococcus faecalis isolates were genetically distinct from the human isolates; identical PFGE patterns were observed among plant isolates that fell into one of six clonal groups. These findings suggest that (i) E. coli and enterococci occurrence in pitcher plants is rather common in the bog studied, although their originating source is unclear, and (ii) the pitcher fluid contains adequate nutrients, especially carbon and energy sources, to promote the growth of indicator bacteria; however, under natural conditions, the biotic factors (e.g., competition for nutrients) may restrict their growth.Key words: indicator bacteria, environmental occurrence, microcosm, pitcher plant fluid, temperate bog.

scholarly journals IN VITRO PLANLET INDUCTION OF TROPICAL PITCHER PLANT (NEPENTHES AMPULLARIA JACK) BY VARIOUS THIAMIN AND BENZYL AMINO PURINE CONCENTRATE

Agrivet ◽  
2021 ◽  
Vol 25 (2) ◽  
pp. 78
Author(s):  
Arwinda Dinar Cryssanti ◽  
Ari Wijayani ◽  
Endah Wahyurini
Keyword(s):  
Plant Tissue ◽  
Design Method ◽  
Isolation Method ◽  
Pitcher Plant ◽  
Random Design ◽  
Tropical Plant ◽  
Benzyl Amino Purine

Nepenthes is one of Indonesian tropical plant as a biodiversity source that endangered from its extinction. One of the effort to prevent its extinction by using plant tissue isolation method on Nepenthes multiplication are needed. This research was aimed to determine the best interaction between Thiamin and benzyl amino purine concentration on in vitro Nepenthes planlet growth, Thiamin best concentration on in vitro Nepenthes planlet growth, and benzyl amino purine best concentration on in vitro Nepenthes planlet growth. The research was conducted in Agriculture Department Biotechnology Laboratory Universitas Pembangunan Nasional “Veteran” Yogyakarta on January – April 2018. Completed Random Design method with 2 factors was used in this research. The first factor was various concentration of Thiamin with 8 ppm, 10 ppm, and 12 ppm. The second factors was various concentration of benzyl amino purine with 0,5 ppm, 1 ppm, and 1,5 ppm. Every combination was repeated 3 times. The result of this research showed that no interaction was found in every Thiamin and benzyl amino purine various concentration on in vitro Nepenthes planlet. The usage of 10 ppm Thiamin concentration (T2) produce the best results on sprout’s amount and sprout’s height. On 1 ppm of benzyl amino purine concentration produce the best result on sprout’s amount, sptout’s height, and amount of leaves.Keyword: Nepenthes, Thiamin, Benzyl Amino Purine

scholarly journals Effect of cytokinin and cultivar on shoot formation of Gerbera jamesonii in vitro.

1982 ◽  
Vol 30 (4) ◽  
pp. 341-346 ◽  
Author(s):  
R.L.M. Pierik ◽  
H.H.M. Steegmans ◽  
J.A.M. Verhaegh ◽  
A.N. Wouters
Keyword(s):  
Clonal Propagation ◽  
Callus Formation ◽  
Shoot Formation ◽  
Optimum Level ◽  
Gerbera Jamesonii ◽  
Axillary Shoots ◽  
Cytokinin Level ◽  
Leaf Weight ◽  
Axillary Branching

The success of gerbera clonal propagation in vitro, using either capitulum explants or subcultured shoots in trials with up to 28 cvs, depended both on the cv. and on the cytokinin level in the medium. With capitulum explants, shoot formation was very low for some cvs regardless of the level of BA (5, 10 or 20 mg/l) whereas other cvs had individual optimum BA levels. Axillary branching of subcultured shoots differed between cvs and between levels of kinetin (1, 5 or 10 mg/l) in the medium. The optimum level for the highest quality shoots (highest leaf weight/shoot, no leaf malformation and no callus formation) was not always the same as the level producing the highest number of axillary shoots. Results are tabulated for each cv. (Abstract retrieved from CAB Abstracts by CABI’s permission)

PCR based molecular characterization of Nepenthes khasiana Hook. f.—pitcher plant

2009 ◽  
Vol 56 (8) ◽  
pp. 1183-1193 ◽  
Author(s):  
B. S. Bhau ◽  
K. Medhi ◽  
T. Sarkar ◽  
S. P. Saikia
Keyword(s):  
Molecular Characterization ◽  
Pitcher Plant ◽  
Nepenthes Khasiana

scholarly journals Dipteran larvae and microbes facilitate nutrient sequestration in the Nepenthes gracilis pitcher plant host

2017 ◽  
Vol 13 (3) ◽  
pp. 20160928 ◽  
Author(s):  
Weng Ngai Lam ◽  
Kwek Yan Chong ◽  
Ganesh S. Anand ◽  
Hugh Tiang Wah Tan
Keyword(s):  
Inorganic Nitrogen ◽  
Plant Host ◽  
Pitcher Plant ◽  
Prey Protein ◽  
Nitrogen Sequestration ◽  
The Relationship ◽  
Nutrient Sequestration ◽  
Nepenthes Gracilis

The fluid-containing traps of Nepenthes carnivorous pitcher plants (Nepenthaceae) are often inhabited by organisms known as inquilines. Dipteran larvae are key components of such communities and are thought to facilitate pitcher nitrogen sequestration by converting prey protein into inorganic nitrogen, although this has never been demonstrated in Nepenthes . Pitcher fluids are also inhabited by microbes, although the relationship(s) between these and the plant is still unclear. In this study, we examined the hypothesis of digestive mutualism between N. gracilis pitchers and both dipteran larvae and fluid microbes. Using dipteran larvae, prey and fluid volumes mimicking in situ pitcher conditions, we conducted in vitro experiments and measured changes in available fluid nitrogen in response to dipteran larvae and microbe presence. We showed that the presence of dipteran larvae resulted in significantly higher and faster releases of ammonium and soluble protein into fluids in artificial pitchers, and that the presence of fluid microbes did likewise for ammonium. We showed also that niche segregation occurs between phorid and culicid larvae, with the former fragmenting prey carcasses and the latter suppressing fluid microbe levels. These results clarify the relationships between several key pitcher-dwelling organisms, and show that pitcher communities facilitate nutrient sequestration in their host.

Sign in / Sign up

Export Citation Format

Share Document