scholarly journals An easy direct PCR method for detecting Pseudomonas syringae pv. theae in samples of bacterial shoot blight collected from tea plants (Camellia sinensis (L) O. Kuntze)

2016 ◽  
Vol 58 (0) ◽  
pp. 91-93
Author(s):  
Mizue Tsuji ◽  
Masayuki Togawa ◽  
Shunsuke Tomita ◽  
Yuichi Takikawa
Keyword(s):  
Pseudomonas Syringae ◽  
Camellia Sinensis ◽  
Direct Pcr ◽  
Shoot Blight ◽  
Pcr Method ◽  
Tea Plants

scholarly journals Biochemical characterization of specific Alanine Decarboxylase (AlaDC) and its ancestral enzyme Serine Decarboxylase (SDC) in tea plants (Camellia sinensis)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Peixian Bai ◽  
Liyuan Wang ◽  
Kang Wei ◽  
Li Ruan ◽  
Liyun Wu ◽  
...  
Keyword(s):  
Gene Duplication ◽  
Camellia Sinensis ◽  
Biochemical Characterization ◽  
Specific Activity ◽  
Protein Sequences ◽  
Biochemical Properties ◽  
High Similarity ◽  
New Gene ◽  
Tea Plants

Abstract Background Alanine decarboxylase (AlaDC), specifically present in tea plants, is crucial for theanine biosynthesis. Serine decarboxylase (SDC), found in many plants, is a protein most closely related to AlaDC. To investigate whether the new gene AlaDC originate from gene SDC and to determine the biochemical properties of the two proteins from Camellia sinensis, the sequences of CsAlaDC and CsSDC were analyzed and the two proteins were over-expressed, purified, and characterized. Results The results showed that exon-intron structures of AlaDC and SDC were quite similar and the protein sequences, encoded by the two genes, shared a high similarity of 85.1%, revealing that new gene AlaDC originated from SDC by gene duplication. CsAlaDC and CsSDC catalyzed the decarboxylation of alanine and serine, respectively. CsAlaDC and CsSDC exhibited the optimal activities at 45 °C (pH 8.0) and 40 °C (pH 7.0), respectively. CsAlaDC was stable under 30 °C (pH 7.0) and CsSDC was stable under 40 °C (pH 6.0–8.0). The activities of the two enzymes were greatly enhanced by the presence of pyridoxal-5′-phosphate. The specific activity of CsSDC (30,488 IU/mg) was 8.8-fold higher than that of CsAlaDC (3467 IU/mg). Conclusions Comparing to CsAlaDC, its ancestral enzyme CsSDC exhibited a higher specific activity and a better thermal and pH stability, indicating that CsSDC acquired the optimized function after a longer evolutionary period. The biochemical properties of CsAlaDC might offer reference for theanine industrial production.

Direct PCR-based genetic mapping of rice telomeric repeat associated sequences

Genome ◽  
1998 ◽  
Vol 41 (2) ◽  
pp. 193-198 ◽  
Author(s):  
Lishuang Shen ◽  
Lihuang Zhu
Keyword(s):  
Genetic Mapping ◽  
Oryza Sativa L ◽  
Telomeric Repeat ◽  
Doubled Haploid Population ◽  
Direct Pcr ◽  
Japonica Variety ◽  
Rapd Primer ◽  
Associated Sequences ◽  
Haploid Population ◽  
Pcr Method

Direct PCR-based genetic mapping of telomeric repeat associated sequences (TASs) was achieved using a RAPD primer mediated asymmetric PCR method. Twenty-two TAS loci were mapped in a rice doubled haploid population derived from a cross between an indica variety (Zhaiyeqing8) and a japonica variety (Jingxi17). Of these, 11 loci were mapped to the most distal position of seven chromosome arms and lengthened the linkage groups by 7.4-22.6 cM, five were mapped to the approximate positions of the centromeric regions, and six were mapped to other interstitial chromosomal regions.Key words: rice, Oryza sativa L., genetic mapping, telomeric repeat, telomeric repeat associated sequences, RAPD primer mediated PCR.

scholarly journals The Protective Effect of Exogenous Putrescine in the Response of Tea Plants (Camellia sinensis) to Salt Stress

HortScience ◽  
2018 ◽  
Vol 53 (11) ◽  
pp. 1640-1646 ◽  
Author(s):  
Fei Xiong ◽  
Jieren Liao ◽  
Yuanchun Ma ◽  
Yuhua Wang ◽  
Wanping Fang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Camellia Sinensis ◽  
Effective Means ◽  
Plant Performance ◽  
Photosynthetic Parameters ◽  
Activity Levels ◽  
Salinity Treatment ◽  
Ros Scavenging ◽  
Stress Levels ◽  
Tea Plants

Camellia sinensis cultivars were treated with 5 mm putrescine (Put) under a range of sodium chloride (NaCl) concentrations. Plant performance, as indicated by various indicators associated with plant growing condition such as photosynthetic parameters and polyamine (PA) contents, especially the content of Put, was improved by the treatment. The extent of both Na+ accumulation and K+ loss increased. The activity levels of the antioxidant enzymes related to salt stress, such as superoxide dismutase (SOD), peroxidase (POD), and catalase, were significantly altered with different salt stress levels and showed a decrease in the general trend. Besides, tea polyphenols, the tea quality indicator, increased during the salinity treatment but decreased when we applied Put. These findings suggest that treatment with Put might constitute an effective means for alleviating salinity stress–induced injury through its positive effect on photosynthetic efficiency and for controlling reactive oxygen species (ROS) scavenging ability under appropriate salt stress levels.

scholarly journals Molecular Identification and Characterization of Hydroxycinnamoyl Transferase in Tea Plants (Camellia sinensis L.)

2018 ◽  
Vol 19 (12) ◽  
pp. 3938 ◽  
Author(s):  
Chi-Hui Sun ◽  
Chin-Ying Yang ◽  
Jason Tzen
Keyword(s):  
Abiotic Stress ◽  
Amino Acid ◽  
Camellia Sinensis ◽  
Polymerase Chain Reaction Analysis ◽  
Coffea Canephora ◽  
Tea Plant ◽  
Amino Acid Sequences ◽  
Hibiscus Cannabinus ◽  
Expression Level ◽  
Tea Plants

Tea (Camellia sinensis L.) contains abundant secondary metabolites, which are regulated by numerous enzymes. Hydroxycinnamoyl transferase (HCT) is involved in the biosynthesis pathways of polyphenols and flavonoids, and it can catalyze the transfer of hydroxyconnamoyl coenzyme A to substrates such as quinate, flavanol glycoside, or anthocyanins, thus resulting in the production of chlorogenic acid or acylated flavonol glycoside. In this study, the CsHCT gene was cloned from the Chin-Shin Oolong tea plant, and its protein functions and characteristics were analyzed. The full-length cDNA of CsHCT contains 1311 base pairs and encodes 436 amino acid sequences. Amino acid sequence was highly conserved with other HCTs from Arabidopsis thaliana, Populus trichocarpa, Hibiscus cannabinus, and Coffea canephora. Quantitative real-time polymerase chain reaction analysis showed that CsHCT is highly expressed in the stem tissues of both tea plants and seedlings. The CsHCT expression level was relatively high at high altitudes. The abiotic stress experiment suggested that low temperature, drought, and high salinity induced CsHCT transcription. Furthermore, the results of hormone treatments indicated that abscisic acid (ABA) induced a considerable increase in the CsHCT expression level. This may be attributed to CsHCT involvement in abiotic stress and ABA signaling pathways.

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