Direct PCR-based genetic mapping of rice telomeric repeat associated sequences

Genome ◽  
1998 ◽  
Vol 41 (2) ◽  
pp. 193-198 ◽  
Author(s):  
Lishuang Shen ◽  
Lihuang Zhu
Keyword(s):  
Genetic Mapping ◽  
Oryza Sativa L ◽  
Telomeric Repeat ◽  
Doubled Haploid Population ◽  
Direct Pcr ◽  
Japonica Variety ◽  
Rapd Primer ◽  
Associated Sequences ◽  
Haploid Population ◽  
Pcr Method

Direct PCR-based genetic mapping of telomeric repeat associated sequences (TASs) was achieved using a RAPD primer mediated asymmetric PCR method. Twenty-two TAS loci were mapped in a rice doubled haploid population derived from a cross between an indica variety (Zhaiyeqing8) and a japonica variety (Jingxi17). Of these, 11 loci were mapped to the most distal position of seven chromosome arms and lengthened the linkage groups by 7.4-22.6 cM, five were mapped to the approximate positions of the centromeric regions, and six were mapped to other interstitial chromosomal regions.Key words: rice, Oryza sativa L., genetic mapping, telomeric repeat, telomeric repeat associated sequences, RAPD primer mediated PCR.

scholarly journals Genetic Analysis of Agronomic Traits and Grain Iron and Zinc Concentrations in a Doubled Haploid Population of Rice (Oryza sativa L.)

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mark Ian C. Calayugan ◽  
Andrea Kariza Formantes ◽  
Amery Amparado ◽  
Gwen Iris Descalsota-Empleo ◽  
Chau Thanh Nha ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Genetic Analysis ◽  
Doubled Haploid ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Doubled Haploid Population ◽  
Iron And Zinc ◽  
Zinc Concentrations ◽  
Haploid Population

Phenotypic Characterization and Genetic Mapping of an Increased Stigma Mutant in Rice (Oryza sativa L.)

2011 ◽  
Vol 37 (10) ◽  
pp. 1779-1784
Author(s):  
Ming-Jing ZHOU ◽  
Yong WEN ◽  
Shuang-Cheng LI ◽  
Cheng-Bo LI ◽  
Man-Hua ZHANG ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Genetic Mapping ◽  
Oryza Sativa L ◽  
Phenotypic Characterization

scholarly journals Novel QTL Associated with Shoot Branching Identified in Doubled Haploid Rice (Oryza sativa L.) under Low Nitrogen Cultivation

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 745
Author(s):  
Youngho Kwon ◽  
Nkulu Rolly Kabange ◽  
Ji-Yun Lee ◽  
So-Myeong Lee ◽  
Jin-Kyung Cha ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Phenotypic Variation ◽  
Growth And Development ◽  
Oryza Sativa L ◽  
Tiller Number ◽  
Yield Potential ◽  
Doubled Haploid Population ◽  
Shoot Branching ◽  
Normal Nitrogen ◽  
Low Nitrogen

Shoot branching is considered as an important trait for the architecture of plants and contributes to their growth and productivity. In cereal crops, such as rice, shoot branching is controlled by many factors, including phytohormones signaling networks, operating either in synergy or antagonizing each other. In rice, shoot branching indicates the ability to produce more tillers that are essential for achieving high productivity and yield potential. In the present study, we evaluated the growth and development, and yield components of a doubled haploid population derived from a cross between 93-11 (P1, indica) and Milyang352 (P2, japonica), grown under normal nitrogen and low nitrogen cultivation open field conditions. The results of the phenotypic evaluation indicated that parental lines 93-11 (P1, a high tillering indica cultivar) and Milyang352 (P2, a low tillering japonica cultivar) showed distinctive phenotypic responses, also reflected in their derived population. In addition, the linkage mapping and quantitative trait locus (QTL) analysis detected three QTLs associated with tiller number on chromosome 2 (qTNN2-1, 130 cM, logarithm of the odds (LOD) 4.14, PVE 14.5%; and qTNL2-1, 134 cM, LOD: 6.05, PVE: 20.5%) and chromosome 4 (qTN4-1, 134 cM, LOD 3.92, PVE 14.5%), with qTNL2-1 having the highest phenotypic variation explained, and the only QTL associated with tiller number under low nitrogen cultivation conditions, using Kompetitive Allele-Specific PCR (KASP) and Fluidigm markers. The additive effect (1.81) of qTNL2-1 indicates that the allele from 93-11 (P1) contributed to the observed phenotypic variation for tiller number under low nitrogen cultivation. The breakthrough is that the majority of the candidate genes harbored by the QTLs qTNL2-1 and qTNN4-1 (here associated with the control of shoot branching under low and normal nitrogen cultivation, respectively), were also proposed to be involved in plant stress signaling or response mechanisms, with regard to their annotations and previous reports. Therefore, put together, these results would suggest that a possible crosstalk exists between the control of plant growth and development and the stress response in rice.

Mapping QTL controlling agronomic traits in a doubled haploid population of winter oilseed rape (Brassica napus L.)

2018 ◽  
Vol 97 (5) ◽  
pp. 1389-1406 ◽  
Author(s):  
Farshad Fattahi ◽  
Barat Ali Fakheri ◽  
Mahmood Solouki ◽  
Christian Möllers ◽  
Abbas Rezaizad
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Agronomic Traits ◽  
Doubled Haploid Population ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
Mapping Qtl ◽  
Haploid Population

scholarly journals Genetic effects of F1 pollen sterility genes S-b, S-d and S-e in rice (Oryza sativa L.)

2019 ◽  
Vol 55 (No. 2) ◽  
pp. 55-60
Author(s):  
Mingsong Jiang ◽  
Jiandi Xu ◽  
Feng Chen ◽  
Wenyin Zhu
Keyword(s):  
Target Genes ◽  
Oryza Sativa L ◽  
Genetic Effect ◽  
Pollen Sterility ◽  
Experimental Population ◽  
Substitution Lines ◽  
Japonica Variety ◽  
Chromosome Segment Substitution Lines ◽  
Selection For ◽  
Sterility Genes

An experimental population commonly used in genetic analyses of gene or quantitative trait loci (QTLs) in rice is chromosome segment substitution lines (CSSLs). In the present study, with the typical indica variety Guangluai 4 as a donor and japonica variety Taichung 65 as a recipient, seven CSSLs carrying F<sub>1 </sub>pollen sterility genes S-b, S-d, S-e, S-b/S-d, S-b/S-e, S-d/S-e, and S-b/S-d/S-e were obtained by specific selection for the target genes, non-specific selection for the genome of the recurrent parents in four backcross populations (BC<sub>1</sub>F<sub>2</sub>, BC<sub>2</sub>F<sub>2</sub>, BC<sub>3</sub>F<sub>2</sub> and BC<sub>3</sub>F<sub>3</sub>). We evaluated the genetic effect of the F<sub>1</sub> pollen sterility genes using 35 F<sub>1 </sub>hybrid individuals in crosses derived from CSSLs and Taichung 65. Pollen fertility of F<sub>1</sub> hybrid plants was observed and the results indicated that the single genes S-b, S-d and S-e can cause 67.7%, 14.6% and 53.2% of pollen sterility, respectively. Multiple genes S-b/S-d, S-b/S-e, S-d/S-e, and S-b/S-d/S-e can cause 76.6%, 85%, 68.7%, and 93% of pollen sterility, respectively.  

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