scholarly journals Aluminum toxicity and Ca depletion may enhance cell death of tobacco cells via similar syndrome

2008 ◽  
Vol 3 (5) ◽  
pp. 290-295 ◽  
Author(s):  
Refat Abdel-Basset ◽  
Hideaki Matsumoto
Keyword(s):  
Cell Death ◽  
Aluminum Toxicity ◽  
Tobacco Cells ◽  
Ca Depletion

scholarly journals Induction of Hypersensitive Cell Death by a Fungal Protein in Cultures of Tobacco Cells

1998 ◽  
Vol 11 (2) ◽  
pp. 115-123 ◽  
Author(s):  
Akira Yano ◽  
Kaoru Suzuki ◽  
Hirofumi Uchimiya ◽  
Hideaki Shinshi
Keyword(s):  
Cell Death ◽  
Specific Interaction ◽  
Trichoderma Viride ◽  
Defense Responses ◽  
Hypersensitive Reaction ◽  
Hypersensitive Cell Death ◽  
Tobacco Cells ◽  
Bright Yellow ◽  
Cell Death Program ◽  
Cellular Signal

Treatment of suspension-cultured tobacco (Nicotiana tabacum cv. Xanthi) cells (line XD6S) with fungal proteinaceous elicitors, namely, xylanase (EC 3.2.1.8) from Trichoderma viride (TvX) and xylanase from T. reesei (TrX), induced shrinkage of the cytoplasm, condensation of the nucleus, and, finally, cell death, which were accompanied by typical defense responses that included an oxidative burst and expression of defense genes. A Ca2+ channel blocker, Gd3+, inhibited the typical response of XD6S cells to TvX, which resembled the hypersensitive reaction (HR). These results suggested that the influx of Ca2+ ions plays an important role as a secondary signal. The HR was not observed in TvX-treated tobacco cells (line BY-2) derived from cv. Bright Yellow 2. This result suggests that key features of cultivar-specific interaction can be observed in cultures of tobacco cells. Xylanase from Bacillus circulans (BcX) and B. subtilis (BsX), which has enzymatic properties similar to those of TvX but an amino acid sequence different from that of TvX, did not induce the HR-like response in XD6S cells. These results suggest that the elicitor action of TvX is not due to its ability to hydrolyze cell walls but requires the TvX-specific recognition factors in plant cells. Thus, TvX-induced cell death was not due to some general toxic effect, but seems to be mediated by the activation of a specific cellular signal-transduction cascade that converges with a pathway that activates the intracellular cell death program.

scholarly journals Phenylpropanoids as a Protectant of Aluminum Toxicity in Cultured Tobacco Cells

1998 ◽  
Vol 39 (9) ◽  
pp. 950-957 ◽  
Author(s):  
Y. Yamamoto ◽  
A. Hachiya ◽  
H. Hamada ◽  
H. Matsumoto
Keyword(s):  
Aluminum Toxicity ◽  
Tobacco Cells

Heat-induced oxidative activity protects suspension-cultured plant cells from low temperature damage

2006 ◽  
Vol 33 (1) ◽  
pp. 67 ◽  
Author(s):  
Andrew C. Allan ◽  
Ratnasiri Maddumage ◽  
Joanne L. Simons ◽  
Samuel O. Neill ◽  
Ian B. Ferguson
Keyword(s):  
Heat Treatment ◽  
Cell Death ◽  
Low Temperature ◽  
Real Time ◽  
Peroxidase Activity ◽  
Low Temperatures ◽  
Temperature Tolerance ◽  
H2o2 Production ◽  
Tobacco Cells ◽  
Pre Treatment

A short heat pre-treatment (1 h at 38°C) was found to protect both suspension-cultured apple fruit cells and tobacco cells from cold-induced cell death. Tobacco cells were more sensitive to low temperatures than apple cells, with significant cell death after 48 h at 0 or –2°C. Real-time measurements of H2O2 levels during the heat pre-treatment revealed a substantial burst of this reactive oxygen species in both cell types. Real-time and longer-term measurements also showed a large burst of H2O2 production from tobacco cells, but not apple cells, when exposed to low temperatures. Lower temperatures reduced levels of peroxidase activity (both total and intracellular), with the heat pre-treatment preventing some of the cold-induced reduction of this activity in both apple and tobacco cells. The greater sensitivity to low temperature of the tobacco cells may be related to higher H2O2 production, with the heat treatment maintaining higher peroxidase activity. The lesser sensitivity of the apple cells may be due to the lack of a H2O2 burst and maintenance of peroxidase activity by the heat treatment. These results support a role for oxidative metabolism in the beneficial effects of heat in inducing low temperature tolerance.

Mitochondrial alterations related to programmed cell death in tobacco cells under aluminium stress

2008 ◽  
Vol 331 (8) ◽  
pp. 597-610 ◽  
Author(s):  
Sanjib Kumar Panda ◽  
Yoko Yamamoto ◽  
Hideki Kondo ◽  
Hideaki Matsumoto
Keyword(s):  
Cell Death ◽  
Programmed Cell Death ◽  
Tobacco Cells ◽  
Mitochondrial Alterations

Nuclear calcium controls the apoptotic-like cell death induced by d-erythro-sphinganine in tobacco cells

Cell Calcium ◽  
2010 ◽  
Vol 47 (1) ◽  
pp. 92-100 ◽  
Author(s):  
Christophe Lachaud ◽  
Daniel Da Silva ◽  
Valérie Cotelle ◽  
Patrice Thuleau ◽  
Tou Cheu Xiong ◽  
...  
Keyword(s):  
Cell Death ◽  
Tobacco Cells ◽  
Nuclear Calcium
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