Heat-induced oxidative activity protects suspension-cultured plant cells from low temperature damage

2006 ◽  
Vol 33 (1) ◽  
pp. 67 ◽  
Author(s):  
Andrew C. Allan ◽  
Ratnasiri Maddumage ◽  
Joanne L. Simons ◽  
Samuel O. Neill ◽  
Ian B. Ferguson
Keyword(s):  
Heat Treatment ◽  
Cell Death ◽  
Low Temperature ◽  
Real Time ◽  
Peroxidase Activity ◽  
Low Temperatures ◽  
Temperature Tolerance ◽  
H2o2 Production ◽  
Tobacco Cells ◽  
Pre Treatment

A short heat pre-treatment (1 h at 38°C) was found to protect both suspension-cultured apple fruit cells and tobacco cells from cold-induced cell death. Tobacco cells were more sensitive to low temperatures than apple cells, with significant cell death after 48 h at 0 or –2°C. Real-time measurements of H2O2 levels during the heat pre-treatment revealed a substantial burst of this reactive oxygen species in both cell types. Real-time and longer-term measurements also showed a large burst of H2O2 production from tobacco cells, but not apple cells, when exposed to low temperatures. Lower temperatures reduced levels of peroxidase activity (both total and intracellular), with the heat pre-treatment preventing some of the cold-induced reduction of this activity in both apple and tobacco cells. The greater sensitivity to low temperature of the tobacco cells may be related to higher H2O2 production, with the heat treatment maintaining higher peroxidase activity. The lesser sensitivity of the apple cells may be due to the lack of a H2O2 burst and maintenance of peroxidase activity by the heat treatment. These results support a role for oxidative metabolism in the beneficial effects of heat in inducing low temperature tolerance.

scholarly journals Concurrent effects of cold and hyperkalaemia cause insect chilling injury

2015 ◽  
Vol 282 (1817) ◽  
pp. 20151483 ◽  
Author(s):  
Heath A. MacMillan ◽  
Erik Baatrup ◽  
Johannes Overgaard
Keyword(s):  
Cell Death ◽  
Low Temperature ◽  
Cold Exposure ◽  
Low Temperatures ◽  
Locusta Migratoria ◽  
Chilling Injury ◽  
Distribution Patterns ◽  
Temperature Tolerance ◽  
Migratory Locust

Chilling injury and death are the ultimate consequence of low temperature exposure for chill susceptible insects, and low temperature tolerance is considered one of the most important factors determining insect distribution patterns. The physiological mechanisms that cause chilling injury are unknown, but chronic cold exposure that causes injury is consistently associated with elevated extracellular [K + ], and cold tolerant insects possess a greater capacity to maintain ion balance at low temperatures. Here, we use the muscle tissue of the migratory locust ( Locusta migratoria ) to examine whether chill injury occurs during cold exposure or following return to benign temperature and we specifically examine if elevated extracellular [K + ], low temperature, or a combination thereof causes cell death. We find that in vivo chill injury occurs during the cold exposure (when extracellular [K + ] is high) and that there is limited capacity for repair immediately following the cold stress. Further, we demonstrate that that high extracellular [K + ] causes cell death in situ , but only when experienced at low temperatures. These findings strongly suggest that that the ability to maintain ion (particularly K + ) balance is critical to insect low temperature survival, and highlight novel routes of study in the mechanisms regulating cell death in insects in the cold.

scholarly journals Characteristics of Black Ginseng (Panax ginseng C.A. Mayer) Production Using Ginseng Stored at Low Temperature after Harvest

Metabolites ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 98
Author(s):  
Hyo Bin Oh ◽  
Ji Won Lee ◽  
Da Eun Lee ◽  
Soo Chang Na ◽  
Da Eun Jeong ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Low Temperature ◽  
Panax Ginseng ◽  
Low Temperatures ◽  
Sugar Content ◽  
Base Material ◽  
Free Sugar ◽  
Drying Process ◽  
Heat Treated ◽  
Black Ginseng

Ginseng processing often involves multiple drying and heat treatments. Ginseng is typically processed within one week of harvesting or is stored at low temperatures to prevent spoilage. Black ginseng (BG) is manufactured by repeating the heat treatment and drying process of ginseng several times. We compared the suitability of low-temperature stored ginseng (SG) and harvested ginseng (HG) as the components for black ginseng production. SG and HG were processed into black ginseng and the appearance change, free sugar content, and benzo[a]pyrene (BAP) content were observed. Appearance observations showed the SG to be suitable in terms of quality when heat-treated at a temperature of 95 ℃ or higher. The BAP content of the SG increased significantly as the steaming process was repeated. A maximum BAP concentration of 5.31 ± 1.12 μg/kg was measured in SG steamed from 2 to 5 times, making it unsuitable for processing into BG. SG and HG showed similar trends in the content of sucrose, fructose, and glucose during steaming. This study aimed to facilitate the proper choice of base material to improve the safety of black ginseng by limiting BAP production during processing.

Effects of temperature and salinity on survival of young-of-the-year Hudson River striped bass (Morone saxatilis): implications for optimal overwintering habitats

2002 ◽  
Vol 59 (5) ◽  
pp. 787-795 ◽  
Author(s):  
Thomas P Hurst ◽  
David O Conover
Keyword(s):  
Body Size ◽  
Low Temperature ◽  
Striped Bass ◽  
Low Temperatures ◽  
Morone Saxatilis ◽  
Hudson River ◽  
Temperature Tolerance ◽  
Low Temperature Tolerance ◽  
Energetic State ◽  
Young Of The Year

We examined the role of salinity, body size, and energetic state in determining low temperature tolerance of young-of-the-year (YOY) striped bass (Morone saxatilis) and used this information to map optimal overwintering habitat in the Hudson River estuary. A long-term experiment compared survival at 15 ppt and 30 ppt. In additional experiments, winter-acclimated fish were exposed to temperature declines (2.3°C·day–1 to 1°C·week–1) at salinities from 0 ppt to 35 ppt. Highest survival at low temperatures was consistently observed at intermediate salinities. These results suggest that the observed distribution of overwintering striped bass is related to physiological constraints on osmo regulatory ability at low temperatures. Low temperature tolerance appeared unrelated to body size and energetic state. Salinity profiles were used to describe the location and extent of optimal wintering habitats under various hydrographic regimes. The location of optimal habitats was displaced by over 27 km along the river axis because of variation in salinity regime. Changes in the availability of optimal habitat may be responsible for variation in recruitment to the Hudson River population. These results demonstrate the need to consider a holistic approach encompassing all seasons of the year in assessing habitat requirements of fishes.

scholarly journals Joint Transcriptomic and Metabolomic Analysis Reveals the Mechanism of Low Temperature Tolerance in Hosta Ventricosa

2021 ◽  
Author(s):  
QianQian Zhuang ◽  
Shaopeng Chen ◽  
ZhiXin Jua ◽  
Yao Yue
Keyword(s):  
Signal Transduction ◽  
Low Temperature ◽  
Low Temperatures ◽  
Temperature Tolerance ◽  
Differentially Expressed ◽  
Metabolomic Analysis ◽  
Temperature Changes ◽  
Low Temperature Tolerance ◽  
Temperature Damage ◽  
Hosta Ventricosa

Abstract Background: Hosta ventricosa is a robust ornamental perennial plant that can tolerate low temperatures, and which is widely used in urban landscaping design in Northeast China. However, the mechanism of cold stress tolerance in this species is unclear. Methods:This study used a combination of transcriptomic and metabolomic analysis to explore the mechanism of low temperature tolerance in H. ventricosa.Results: A total of 12 059 differentially expressed genes (DEGs) and 131 differentially expressed metabolites were obtained, which were mainly concentrated in the signal transduction and phenylpropanoid metabolic pathways. In the process of low temperature signal transduction, H. ventricosa is mainly through the ion channels on the three cell membranes of COLD, CNGCs and CRLK to transmit Ca2+ inside and outside the cell to sense temperature changes, and stimulate SCRM to combine with DREB through the MAPK signal pathway and Ca2+ signal sensors such as CBL. Strengthen the low temperature resistance of H. ventricosa. The pathways of phenylpropanoid and flavonoid metabolism represent the main mechanism of low temperature tolerance in this species. The plant protects itself from low temperature damage by increasing its content of genistein, scopolentin and scopolin. It is speculated that H. ventricosa can also adjust the content ratio of sinapyl alcohol and coniferyl alcohol and thereby alter the morphological structure of its cell walls and so increase its resistance to low temperatures.Conclusions: In H. ventricosa that is subjected to low temperature stress, temperature changes are perceived through COLD, CNGCs and CRLK, and protection from low temperature damage is achieved by an increase in the levels of genistein, scopolentin and scopolin through the pathways of phenylpropanoid biosynthesis and flavonoid biosynthesis.

scholarly journals Induction of Low Temperature Tolerance in Wheat by Pre-Soaking and Parental Treatment with Melatonin

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1192
Author(s):  
Hua Zhang ◽  
Lei Liu ◽  
Zongshuai Wang ◽  
Guozhong Feng ◽  
Qiang Gao ◽  
...  
Keyword(s):  
Low Temperature ◽  
Low Temperatures ◽  
Germination Rate ◽  
Grain Filling ◽  
Temperature Tolerance ◽  
Starch Degradation ◽  
Wheat Seedlings ◽  
Low Temperature Tolerance ◽  
Metabolism Enzymes ◽  
Parental Treatment

Low temperatures seriously depress germination and seedling establishment in wheat and it is of great significance to explore approaches to improve wheat tolerance to low temperatures. In this study, the effects of seed pre-soaking and parental treatment with melatonin on seed germination and low temperature tolerance during the early growing stage in wheat were studied. The results showed that pre-soaking with melatonin increased the germination rate, improved antioxidant capacity and accelerated starch degradation under low temperature, which alleviated low temperature-induced damage to the chloroplasts in coleoptiles of wheat seedlings. Parental melatonin treatment during grain filling stage significantly decreased the grain weight. Seeds from parental melatonin-treated plants showed higher germination rates and higher antioxidant enzyme activity than the control seeds under low temperature. In addition, parental treatment with melatonin modulated the activities of carbohydrate metabolism enzymes, which contributes to enhanced low temperature tolerance in wheat offspring. It was suggested that both seed pre-soaking and parental treatment with melatonin could be the effective approaches for low temperature tolerance induction in wheat.

Peroxidase activity, lignification and promotion of cell death in tobacco cells exposed to static magnetic field

2007 ◽  
Vol 27 (4) ◽  
pp. 435-440 ◽  
Author(s):  
Parviz Abdolmaleki ◽  
Faezeh Ghanati ◽  
Hassan Sahebjamei ◽  
Amir Sabet Sarvestani
Keyword(s):  
Magnetic Field ◽  
Cell Death ◽  
Static Magnetic Field ◽  
Peroxidase Activity ◽  
Tobacco Cells

scholarly journals Light Intensity: The Role Player in Cucumber Response to Cold Stress

Agronomy ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 201
Author(s):  
Tahereh Ashrostaghi ◽  
Sasan Aliniaeifard ◽  
Aida Shomali ◽  
Shiva Azizinia ◽  
Jahangir Abbasi Koohpalekani ◽  
...  
Keyword(s):  
Electron Transport ◽  
Low Temperature ◽  
Light Intensity ◽  
Cold Stress ◽  
Ascorbate Peroxidase ◽  
Peroxidase Activity ◽  
Low Temperatures ◽  
Electron Transport Chain ◽  
Biochemical Traits ◽  
Transport Chain

Low temperatures are a substantial limitation in the geographic distribution of warm-season crops such as cucumber (Cucumis sativus L.). Tolerance to low temperatures varies among different plant species and genotypes when changes in environmental cues occur. Therefore, biochemical and biophysical events should be coordinated to form a physiological response and cope with low temperatures. We examined how light intensity influences the effects of low temperature on photosynthesis and some biochemical traits. We used chlorophyll fluorescence imaging and polyphasic fluorescence transient to analyze cold stress damage by 4 °C. Photosynthetic Photon Flux Densities (PPFDs) of 0, 300, and 600 μmol m−2 s−1, in four accessions of cucumber, were investigated. The results show that the negative effects of cold stress are PPFD-dependent. The adverse effect of cold stress on the electron transport chain is more pronounced in plants exposed to 600 μmol m−2 s−1 than the control and dark-exposed plants, indicated by a disturbance in the electron transport chain and higher energy dissipation. Moreover, biochemical traits, including the H2O2 content, ascorbate peroxidase activity, electrolyte leakage, and water-soluble carbohydrate, increased under low temperature by increasing the PPFD. In contrast, chlorophyll and carotenoid contents decreased under low temperature through PPFD elevation. Low temperature induced a H2O2 accumulation via suppressing ascorbate peroxidase activity in a PPFD-dependent manner. In conclusion, high PPFDs exacerbate the adverse effects of low temperature on the cucumber seedlings.

scholarly journals Dmc1 is a candidate for temperature tolerance during wheat meiosis

2019 ◽  
Vol 133 (3) ◽  
pp. 809-828 ◽  
Author(s):  
Tracie Draeger ◽  
Azahara C. Martin ◽  
Abdul Kader Alabdullah ◽  
Ali Pendle ◽  
María-Dolores Rey ◽  
...  
Keyword(s):  
Low Temperature ◽  
Chromosome Pairing ◽  
Meiotic Recombination ◽  
Low Temperatures ◽  
Chinese Spring ◽  
Deletion Mutant ◽  
Wheat Chromosome ◽  
Temperature Tolerance ◽  
Chromosome Synapsis ◽  
Recombination Gene

Abstract Key message The meiotic recombination gene Dmc1 on wheat chromosome 5D has been identified as a candidate for the maintenance of normal chromosome synapsis and crossover at low and possibly high temperatures. Abstract We initially assessed the effects of low temperature on meiotic chromosome synapsis and crossover formation in the hexaploid wheat (Triticum aestivum L.) variety ‘Chinese Spring’. At low temperatures, asynapsis and chromosome univalence have been observed before in Chinese Spring lines lacking the long arm of chromosome 5D (5DL), which led to the proposal that 5DL carries a gene (Ltp1) that stabilises wheat chromosome pairing at low temperatures. In the current study, Chinese Spring wild type and 5DL interstitial deletion mutant plants were exposed to low temperature in a controlled environment room during a period from premeiotic interphase to early meiosis I. A 5DL deletion mutant was identified whose meiotic chromosomes exhibit extremely high levels of asynapsis and chromosome univalence at metaphase I after 7 days at 13 °C, suggesting that Ltp1 is deleted in this mutant. Immunolocalisation of the meiotic proteins ASY1 and ZYP1 on ltp1 mutants showed that low temperature results in a failure to complete synapsis at pachytene. KASP genotyping revealed that the ltp1 mutant has a 4-Mb deletion in 5DL. Of 41 genes within this deletion region, the strongest candidate for the stabilisation of chromosome pairing at low temperatures is the meiotic recombination gene Dmc1. The ltp1 mutants were subsequently treated at 30 °C for 24 h during meiosis and exhibited a reduced number of crossovers and increased univalence, though to a lesser extent than at 13 °C. We therefore renamed our ltp1 mutant ‘ttmei1’ (temperature-tolerant meiosis 1) to reflect this additional loss of high temperature tolerance.

HAPLOID SELECTION FOR LOW TEMPERATURE TOLERANCE OF TOMATO POLLEN

Genetics ◽  
1982 ◽  
Vol 101 (1) ◽  
pp. 129-137 ◽  
Author(s):  
Daniel Zamir ◽  
Steven D Tanksley ◽  
Richard A Jones
Keyword(s):  
Low Temperature ◽  
Low Temperatures ◽  
Pollen Grains ◽  
Temperature Tolerance ◽  
F1 Hybrid ◽  
Peruvian Andes ◽  
Low Temperature Tolerance ◽  
Selection For ◽  
Chromosome Segments ◽  
Haploid Selection

ABSTRACT Pollen grains were harvested from an interspecific F1 hybrid between the cultivated tomato, Lycopersicon esculentum Mill., and its wild relative Lycopersicon hirsutum Humb. & Bonpl., a low temperature tolerant accession originating from an altitude of 3200 m in the Peruvian Andes. The two species differ for electrophoretically-detectable loci that mark six (possibly seven) of the 12 tomato chromosomes. Isozyme analysis of the BC1 populations derived from controlled pollinations at normal and low temperatures indicates a significant skewing of allelic frequencies favoring two independent chromosome segments of L. hirsutum at low temperatures. The results demonstrate that gametophytic selection for low temperature tolerance of tomato pollen is determined, at least in part, by genes expressed in the haploid pollen.

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