scholarly journals Real-time dynamics of methyl-CpG-binding domain protein 3 and its role in DNA demethylation by fluorescence correlation spectroscopy

Epigenetics ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. 1089-1100 ◽  
Author(s):  
Yi Cui ◽  
Il-Hoon Cho ◽  
Basudev Chowdhury ◽  
Joseph Irudayaraj
Keyword(s):  
Real Time ◽  
Fluorescence Correlation Spectroscopy ◽  
Dna Demethylation ◽  
Binding Domain ◽  
Correlation Spectroscopy ◽  
Time Dynamics ◽  
Fluorescence Correlation ◽  
Domain Protein

Unusual denaturation trajectory of bovine gamma globulin studied by fluorescence correlation spectroscopy

2015 ◽  
Vol 17 (29) ◽  
pp. 19139-19148 ◽  
Author(s):  
Moupriya Nag ◽  
Debajyoti Das ◽  
Diptankar Bandyopadhyay ◽  
Soumen Basak
Keyword(s):  
Electrostatic Interaction ◽  
Fluorescence Correlation Spectroscopy ◽  
Gamma Globulin ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Domain Protein

FCS study of GdnHCl-induced denaturation of the multi-domain protein, gamma globulin, showed unexpected features such as initial compactification followed by electrostatic interaction-driven expansion before disintegration into small fragments.

scholarly journals Live Cell Fluorescence Correlation Spectroscopy with Real Time Photoactivation Feedback

2013 ◽  
Vol 104 (2) ◽  
pp. 573a
Author(s):  
Matthew D. Weitzman ◽  
Chandran R. Sabanayagam ◽  
Kenneth L. van Golen
Keyword(s):  
Real Time ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Live Cell ◽  
Fluorescence Correlation

scholarly journals Single molecule binding of a ligand to a G-protein-coupled receptor in real time using fluorescence correlation spectroscopy, rendered possible by nano-encapsulation in styrene maleic acid lipid particles

Nanoscale ◽  
2020 ◽  
Vol 12 (21) ◽  
pp. 11518-11525 ◽  
Author(s):  
Rachael L. Grime ◽  
Joelle Goulding ◽  
Romez Uddin ◽  
Leigh A. Stoddart ◽  
Stephen J. Hill ◽  
...  
Keyword(s):  
Real Time ◽  
G Protein ◽  
Single Molecule ◽  
Maleic Acid ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
G Protein Coupled Receptor ◽  
Fluorescence Correlation ◽  
Lipid Particles ◽  
G Protein Coupled

Combining the technologies of encapsulation of GPCRs in SMA lipid particles with fluorescence correlation spectroscopy provides a versatile characterisation platform.

scholarly journals Real-Time Monitoring of Alzheimer’s-Related Amyloid Aggregation via Probe Enhancement–Fluorescence Correlation Spectroscopy

2015 ◽  
Vol 6 (9) ◽  
pp. 1503-1508 ◽  
Author(s):  
Yinghua Guan ◽  
Kevin J. Cao ◽  
Adam Cantlon ◽  
Kristyna Elbel ◽  
Emmanuel A. Theodorakis ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Amyloid Aggregation ◽  
Real Time Monitoring ◽  
Fluorescence Correlation

scholarly journals The design of fluorescence correlation spectroscopy for single molecule techniques

2019 ◽  
Author(s):  
◽  
Zenia Norman
Keyword(s):  
Protein Synthesis ◽  
Real Time ◽  
Single Molecule ◽  
Fluorescence Correlation Spectroscopy ◽  
Ribosomal Proteins ◽  
High Speed ◽  
Correlation Spectroscopy ◽  
Experimental Conditions ◽  
High Count ◽  
Fluorescence Correlation

The ribosome is responsible for protein synthesis--a critical process in creating essential proteins for cell survival. Though bulk techniques yield valuable results about the structure of the ribosome, bulk techniques are not ideal in examining ribosomal dynamics and understanding kinetics involved in protein synthesis. On the contrary, the single molecule spectroscopy techniques are ideal in investigating the mechanisms of ribosome dynamics in real-time under equilibrium conditions. The latest advances in single molecule biophysics have opened numerous opportunities in the biological world to study the dynamics of molecules in real-time. Single molecule techniques such as Fluorescence Correlation Spectroscopy (FCS) have opened opportunities to study the ribosome as well as other ribosomal proteins. FCS is used to investigate diffusion coefficients, concentration, and kinetics of biological samples. In this thesis, we address the process of assembling an FCS system, as well as the design of a high-speed correlator. The high-speed correlator allows the software to handle the high data counts that can occur in single molecule experiments. A field-programmable gate arrays (FPGA) dual correlator and a multi-tau correlator are designed to handle the noise and high count rates that can dominate the signal. Also addressed in this thesis are ideal experimental conditions for successfully obtaining and troubleshooting results received from FCS curves.

Sign in / Sign up

Export Citation Format

Share Document