scholarly journals Alternative splice isoforms of small conductance calcium-activated SK2 channels differ in molecular interactions and surface levels

Channels ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 62-75 ◽  
Author(s):  
Elizabeth Storer Scholl ◽  
Antonella Pirone ◽  
Daniel H Cox ◽  
R Keith Duncan ◽  
Michele H Jacob
Keyword(s):  
Alternative Splice ◽  
Molecular Interactions ◽  
Splice Isoforms ◽  
Alternative Splice Isoforms ◽  
Small Conductance

scholarly journals APPRIS: annotation of principal and alternative splice isoforms

2012 ◽  
Vol 41 (D1) ◽  
pp. D110-D117 ◽  
Author(s):  
Jose Manuel Rodriguez ◽  
Paolo Maietta ◽  
Iakes Ezkurdia ◽  
Alessandro Pietrelli ◽  
Jan-Jaap Wesselink ◽  
...  
Keyword(s):  
Alternative Splice ◽  
Splice Isoforms ◽  
Alternative Splice Isoforms

scholarly journals Identification of Novel Alternative Splice Isoforms of Circulating Proteins in a Mouse Model of Human Pancreatic Cancer

2008 ◽  
Vol 69 (1) ◽  
pp. 300-309 ◽  
Author(s):  
Rajasree Menon ◽  
Qing Zhang ◽  
Yan Zhang ◽  
Damian Fermin ◽  
Nabeel Bardeesy ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Mouse Model ◽  
Alternative Splice ◽  
Human Pancreatic Cancer ◽  
Splice Isoforms ◽  
Alternative Splice Isoforms

scholarly journals hnRNP A/B Proteins: An Encyclopedic Assessment of Their Roles in Homeostasis and Disease

Biology ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 712
Author(s):  
Patricia A. Thibault ◽  
Aravindhan Ganesan ◽  
Subha Kalyaanamoorthy ◽  
Joseph-Patrick W. E. Clarke ◽  
Hannah E. Salapa ◽  
...  
Keyword(s):  
Alternative Splice ◽  
Rna Metabolism ◽  
Protein Family ◽  
Hnrnp A1 ◽  
Cell Type ◽  
Cell Homeostasis ◽  
Splice Isoforms ◽  
Alternative Splice Isoforms ◽  
Functional Differences ◽  
Cell Type Specific

The hnRNP A/B family of proteins is canonically central to cellular RNA metabolism, but due to their highly conserved nature, the functional differences between hnRNP A1, A2/B1, A0, and A3 are often overlooked. In this review, we explore and identify the shared and disparate homeostatic and disease-related functions of the hnRNP A/B family proteins, highlighting areas where the proteins have not been clearly differentiated. Herein, we provide a comprehensive assembly of the literature on these proteins. We find that there are critical gaps in our grasp of A/B proteins’ alternative splice isoforms, structures, regulation, and tissue and cell-type-specific functions, and propose that future mechanistic research integrating multiple A/B proteins will significantly improve our understanding of how this essential protein family contributes to cell homeostasis and disease.

scholarly journals Computer-Aided Discovery of Small Molecules Targeting the RNA Splicing Activity of hnRNP A1 in Castration-Resistant Prostate Cancer

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 763 ◽  
Author(s):  
Lavinia Carabet ◽  
Eric Leblanc ◽  
Nada Lallous ◽  
Helene Morin ◽  
Fariba Ghaidi ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Alternative Splice ◽  
Small Molecule ◽  
Rna Binding ◽  
Critical Role ◽  
Hnrnp A1 ◽  
Castration Resistant Prostate Cancer ◽  
Splice Isoforms ◽  
Castration Resistant ◽  
Computer Aided

The heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a versatile RNA-binding protein playing a critical role in alternative pre-mRNA splicing regulation in cancer. Emerging data have implicated hnRNP A1 as a central player in a splicing regulatory circuit involving its direct transcriptional control by c-Myc oncoprotein and the production of the constitutively active ligand-independent alternative splice variant of androgen receptor, AR-V7, which promotes castration-resistant prostate cancer (CRPC). As there is an urgent need for effective CRPC drugs, targeting hnRNP A1 could, therefore, serve a dual purpose of preventing AR-V7 generation as well as reducing c-Myc transcriptional output. Herein, we report compound VPC-80051 as the first small molecule inhibitor of hnRNP A1 splicing activity discovered to date by using a computer-aided drug discovery approach. The inhibitor was developed to target the RNA-binding domain (RBD) of hnRNP A1. Further experimental evaluation demonstrated that VPC-80051 interacts directly with hnRNP A1 RBD and reduces AR-V7 messenger levels in 22Rv1 CRPC cell line. This study lays the groundwork for future structure-based development of more potent and selective small molecule inhibitors of hnRNP A1–RNA interactions aimed at altering the production of cancer-specific alternative splice isoforms.

Rotary Beveling for In Situ Thin-Section Microscopy of Cell Cultures

1981 ◽  
Vol 39 ◽  
pp. 550-551
Author(s):  
Dean A. Handley ◽  
Jack T. Alexander ◽  
Shu Chien
Keyword(s):  
Cell Growth ◽  
Cell Cultures ◽  
Molecular Interactions ◽  
Convenient Method ◽  
Petri Dish ◽  
Simple Method ◽  
Thin Section Microscopy ◽  
Thin Sectioning ◽  
Organic Fluids

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.

Fluorescence of BODIPY Dyes in Gas Phase at near Ambient Conditions

2020 ◽  
Author(s):  
Kseniya A. Mariewskaya ◽  
Denis Larkin ◽  
Yuri Samoilichenko ◽  
Vladimir Korshun ◽  
Alex Ustinov
Keyword(s):  
Gas Phase ◽  
Molecular Interactions ◽  
Atmospheric Pressure ◽  
Fluorescence Spectra ◽  
Visible Range ◽  
Intrinsic Properties ◽  
Ambient Conditions ◽  
Ideal Model ◽  
Molecular Fluorescence ◽  
Tesla Coil

Molecular fluorescence is a phenomenon that is usually observed in condensed phase. It is strongly affected by molecular interactions. The study of fluorescence spectra in the gas phase can provide a nearly-ideal model for the evaluation of intrinsic properties of the fluorophores. Unfortunately, most conventional fluorophores are not volatile enough to allow study of their fluorescence in the gas phase. Here we report very bright gas phase fluorescence of simple BODIPY dyes that can be readily observed at atmospheric pressure using conventional fluorescence instrumentation. To our knowledge, this is the first example of visible range gas phase fluorescence at near ambient conditions. Evaporation of the dye in vacuum allowed us to demonstrate organic molecular electroluminescence in gas discharge excited by electric field produced by a Tesla coil.

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