Novel transgenes for plant resistance to aphids from plant virus-aphid vector molecular interactions

2016 ◽  
Author(s):  
Bryony C. Bonning
Keyword(s):  
Molecular Interactions ◽  
Plant Resistance ◽  
Plant Virus ◽  
Aphid Vector

Toxin delivery by the coat protein of an aphid-vectored plant virus provides plant resistance to aphids

2013 ◽  
Vol 32 (1) ◽  
pp. 102-105 ◽  
Author(s):  
Bryony C Bonning ◽  
Narinder Pal ◽  
Sijun Liu ◽  
Zhaohui Wang ◽  
S Sivakumar ◽  
...  
Keyword(s):  
Coat Protein ◽  
Plant Resistance ◽  
Plant Virus

Molecular Interactions Between a Plant Virus Movement Protein and RNA: Force Spectroscopy Investigation

2004 ◽  
Vol 339 (5) ◽  
pp. 1041-1047 ◽  
Author(s):  
Igor A. Andreev ◽  
Sang Hyon Kim ◽  
Natalia O. Kalinina ◽  
Darya V. Rakitina ◽  
Alexander G. Fitzgerald ◽  
...  
Keyword(s):  
Molecular Interactions ◽  
Plant Virus ◽  
Movement Protein ◽  
Force Spectroscopy ◽  
Virus Movement

scholarly journals In VitroEvidence Supports Membrane Alanyl Aminopeptidase N as a Receptor for a Plant Virus in the Pea Aphid Vector

2015 ◽  
Vol 89 (22) ◽  
pp. 11203-11212 ◽  
Author(s):  
Lucas B. Linz ◽  
Sijun Liu ◽  
Nanasaheb P. Chougule ◽  
Bryony C. Bonning
Keyword(s):  
Plant Virus ◽  
Food Production ◽  
Virus Transmission ◽  
Plant Viruses ◽  
Aminopeptidase N ◽  
Pea Aphid ◽  
Aphid Vector ◽  
Alanyl Aminopeptidase

ABSTRACTInsect-borne plant viruses cause significant agricultural losses and jeopardize sustainable global food production. Although blocking plant virus transmission would allow for crop protection, virus receptors in insect vectors are unknown. Here we identify membrane alanyl aminopeptidase N (APN) as a receptor for pea enation mosaic virus (PEMV) coat protein (CP) in the gut of the pea aphid,Acyrthosiphon pisum, using a far-Western blot method. Pulldown and immunofluorescence binding assays and surface plasmon resonance were used to confirm and characterize CP-APN interaction. PEMV virions and a peptide comprised of PEMV CP fused to a proline-rich hinge (-P-) and green fluorescent protein (CP-P-GFP) specifically bound to APN. Recombinant APN expressed in Sf9 cells resulted in internalization of CP-P-GFP, which was visualized by confocal microscopy; such internalization is an expected hallmark of a functional gut receptor. Finally, in assays with aphid gut-derived brush border membrane vesicles, binding of CP-P-GFP competed with binding of GBP3.1, a peptide previously demonstrated to bind to APN in the aphid gut and to impede PEMV uptake into the hemocoel; this finding supports the hypothesis that GBP3.1 and PEMV bind to and compete for the same APN receptor. Thesein vitrodata combined with previously publishedin vivoexperiments (S. Liu, S. Sivakumar, W. O. Sparks, W. A. Miller, and B. C. Bonning, Virology 401:107–116, 2010,http://dx.doi.org/10.1016/j.virol.2010.02.009) support the identification of APN as the first receptor in a plant virus vector. Knowledge of this receptor will provide for technologies based on PEMV-APN interaction designed to block plant virus transmission and to suppress aphid populations.IMPORTANCEA significant proportion of global food production is lost to insect pests. Aphids, in addition to weakening plants by feeding on their sap, are responsible for transmitting about half of the plant viruses vectored by insects. Growers rely heavily on the application of chemical insecticides to manage both aphids and aphid-vectored plant viral disease. To increase our understanding of plant virus-aphid vector interaction, we providein vitroevidence supporting earlierin vivowork for identification of a receptor protein in the aphid gut called aminopeptidase N, which is responsible for entry of the plant virus pea enation mosaic virus into the pea aphid vector. Enrichment of proteins found on the surface of the aphid gut epithelium resulted in identification of this first aphid gut receptor for a plant virus. This discovery is particularly important since the disruption of plant virus binding to such a receptor may enable the development of a nonchemical strategy for controlling aphid-vectored plant viruses to maximize food production.

Responses to predation risk cues and alarm pheromones affect plant virus transmission by an aphid vector

Oecologia ◽  
2021 ◽  
Author(s):  
Benjamin W. Lee ◽  
Saumik Basu ◽  
Sayanta Bera ◽  
Clare L. Casteel ◽  
David W. Crowder
Keyword(s):  
Predation Risk ◽  
Plant Virus ◽  
Virus Transmission ◽  
Alarm Pheromones ◽  
Aphid Vector

scholarly journals Transcriptome of the Plant Virus Vector Graminella nigrifrons, and the Molecular Interactions of Maize fine streak rhabdovirus Transmission

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
pp. e40613 ◽  
Author(s):  
Yuting Chen ◽  
Bryan J. Cassone ◽  
Xiaodong Bai ◽  
Margaret G. Redinbaugh ◽  
Andrew P. Michel
Keyword(s):  
Molecular Interactions ◽  
Plant Virus ◽  
Virus Vector ◽  
Graminella Nigrifrons ◽  
Plant Virus Vector
Sign in / Sign up

Export Citation Format

Share Document