scholarly journals Stem cell dynamics in mouse hair follicles: A story from cell division counting and single cell lineage tracing

Cell Cycle ◽  
2010 ◽  
Vol 9 (8) ◽  
pp. 1504-1510 ◽  
Author(s):  
Ying V. Zhang ◽  
Brian S. White ◽  
David I. Shalloway ◽  
Tudorita Tumbar
Keyword(s):  
Stem Cell ◽  
Cell Division ◽  
Single Cell ◽  
Cell Lineage ◽  
Hair Follicles ◽  
Lineage Tracing ◽  
Cell Dynamics

scholarly journals Fluctuating methylation clocks for cell lineage tracing at high temporal resolution in human tissues

2022 ◽  
Author(s):  
Calum Gabbutt ◽  
Ryan O. Schenck ◽  
Daniel J. Weisenberger ◽  
Christopher Kimberley ◽  
Alison Berner ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Lineage ◽  
Adult Stem Cell ◽  
Lineage Tracing ◽  
Rapid Expansion ◽  
High Temporal Resolution ◽  
Molecular Clocks ◽  
Cell Renewal ◽  
Cell Dynamics ◽  
Small Intestinal

AbstractMolecular clocks that record cell ancestry mutate too slowly to measure the short-timescale dynamics of cell renewal in adult tissues. Here, we show that fluctuating DNA methylation marks can be used as clocks in cells where ongoing methylation and demethylation cause repeated ‘flip–flops’ between methylated and unmethylated states. We identify endogenous fluctuating CpG (fCpG) sites using standard methylation arrays and develop a mathematical model to quantitatively measure human adult stem cell dynamics from these data. Small intestinal crypts were inferred to contain slightly more stem cells than the colon, with slower stem cell replacement in the small intestine. Germline APC mutation increased the number of replacements per crypt. In blood, we measured rapid expansion of acute leukemia and slower growth of chronic disease. Thus, the patterns of human somatic cell birth and death are measurable with fluctuating methylation clocks (FMCs).

scholarly journals Single-Cell Lineage Tracing Reveals that Oriented Cell Division Contributes to Trabecular Morphogenesis and Regional Specification

Cell Reports ◽  
2016 ◽  
Vol 15 (1) ◽  
pp. 158-170 ◽  
Author(s):  
Jingjing Li ◽  
Lianjie Miao ◽  
David Shieh ◽  
Ernest Spiotto ◽  
Jian Li ◽  
...  
Keyword(s):  
Cell Division ◽  
Single Cell ◽  
Cell Lineage ◽  
Lineage Tracing ◽  
Oriented Cell Division

scholarly journals Single-cell lineage tracing approaches in hematology research: technical considerations

2020 ◽  
Vol 89 ◽  
pp. 26-36 ◽  
Author(s):  
Joana Carrelha ◽  
Dawn S. Lin ◽  
Alejo E. Rodriguez-Fraticelli ◽  
Tiago C. Luis ◽  
Adam C. Wilkinson ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Lineage ◽  
Lineage Tracing ◽  
Technical Considerations

scholarly journals Stem Cell Pluripotency Genes Klf4 and Oct4 Regulate Complex SMC Phenotypic Changes Critical in Late-Stage Atherosclerotic Lesion Pathogenesis

Circulation ◽  
2020 ◽  
Vol 142 (21) ◽  
pp. 2045-2059 ◽  
Author(s):  
Gabriel F. Alencar ◽  
Katherine M. Owsiany ◽  
Santosh Karnewar ◽  
Katyayani Sukhavasi ◽  
Giuseppe Mocci ◽  
...  
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Rna Sequencing ◽  
Late Stage ◽  
Lineage Tracing ◽  
Stem Cell Marker ◽  
Cell Phenotype ◽  
Phenotypic Changes ◽  
Atherosclerotic Lesions ◽  
Single Cell Rna Sequencing

Background: Rupture and erosion of advanced atherosclerotic lesions with a resultant myocardial infarction or stroke are the leading worldwide cause of death. However, we have a limited understanding of the identity, origin, and function of many cells that make up late-stage atherosclerotic lesions, as well as the mechanisms by which they control plaque stability. Methods: We conducted a comprehensive single-cell RNA sequencing of advanced human carotid endarterectomy samples and compared these with single-cell RNA sequencing from murine microdissected advanced atherosclerotic lesions with smooth muscle cell (SMC) and endothelial lineage tracing to survey all plaque cell types and rigorously determine their origin. We further used chromatin immunoprecipitation sequencing (ChIP-seq), bulk RNA sequencing, and an innovative dual lineage tracing mouse to understand the mechanism by which SMC phenotypic transitions affect lesion pathogenesis. Results: We provide evidence that SMC-specific Klf4- versus Oct4-knockout showed virtually opposite genomic signatures, and their putative target genes play an important role regulating SMC phenotypic changes. Single-cell RNA sequencing revealed remarkable similarity of transcriptomic clusters between mouse and human lesions and extensive plasticity of SMC- and endothelial cell-derived cells including 7 distinct clusters, most negative for traditional markers. In particular, SMC contributed to a Myh11 - , Lgals3 + population with a chondrocyte-like gene signature that was markedly reduced with SMC- Klf4 knockout. We observed that SMCs that activate Lgals3 compose up to two thirds of all SMC in lesions. However, initial activation of Lgals3 in these cells does not represent conversion to a terminally differentiated state, but rather represents transition of these cells to a unique stem cell marker gene–positive, extracellular matrix-remodeling, “pioneer” cell phenotype that is the first to invest within lesions and subsequently gives rise to at least 3 other SMC phenotypes within advanced lesions, including Klf4-dependent osteogenic phenotypes likely to contribute to plaque calcification and plaque destabilization. Conclusions: Taken together, these results provide evidence that SMC-derived cells within advanced mouse and human atherosclerotic lesions exhibit far greater phenotypic plasticity than generally believed, with Klf4 regulating transition to multiple phenotypes including Lgals3 + osteogenic cells likely to be detrimental for late-stage atherosclerosis plaque pathogenesis.

Unravelling stem cell dynamics by lineage tracing

2013 ◽  
Vol 14 (8) ◽  
pp. 489-502 ◽  
Author(s):  
Cédric Blanpain ◽  
Benjamin D. Simons
Keyword(s):  
Stem Cell ◽  
Lineage Tracing ◽  
Cell Dynamics

scholarly journals Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Isabella Santi ◽  
Neeraj Dhar ◽  
Djenet Bousbaine ◽  
Yuichi Wakamoto ◽  
John D. McKinney
Keyword(s):  
Cell Division ◽  
Single Cell ◽  
Chromosome Replication ◽  
Cell Dynamics

Su1736 Live Imaging of Single Cell Reveals Single Stem Cell Dynamics in an Organoid Derived From Murine Small Intestine

2013 ◽  
Vol 144 (5) ◽  
pp. S-464
Author(s):  
Nobukatsu Horita ◽  
Kiichiro Tsuchiya ◽  
Shuji Hibiya ◽  
Keita Fukushima ◽  
Yoshihito Kano ◽  
...  
Keyword(s):  
Small Intestine ◽  
Stem Cell ◽  
Single Cell ◽  
Live Imaging ◽  
Cell Dynamics ◽  
Murine Small Intestine

scholarly journals Single-Cell Transcriptomic Analysis Defines Heterogeneity and Transcriptional Dynamics in the Adult Neural Stem Cell Lineage

Cell Reports ◽  
2017 ◽  
Vol 18 (3) ◽  
pp. 777-790 ◽  
Author(s):  
Ben W. Dulken ◽  
Dena S. Leeman ◽  
Stéphane C. Boutet ◽  
Katja Hebestreit ◽  
Anne Brunet
Keyword(s):  
Stem Cell ◽  
Single Cell ◽  
Neural Stem Cell ◽  
Cell Lineage ◽  
Transcriptomic Analysis ◽  
Adult Neural Stem Cell ◽  
Transcriptional Dynamics ◽  
Stem Cell Lineage
Sign in / Sign up

Export Citation Format

Share Document