scholarly journals Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Isabella Santi ◽  
Neeraj Dhar ◽  
Djenet Bousbaine ◽  
Yuichi Wakamoto ◽  
John D. McKinney
Keyword(s):  
Cell Division ◽  
Single Cell ◽  
Chromosome Replication ◽  
Cell Dynamics

scholarly journals Erratum: Single-cell dynamics of the chromosome replication and cell division cycles in mycobacteria

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Isabella Santi ◽  
Neeraj Dhar ◽  
Djenet Bousbaine ◽  
Yuichi Wakamoto ◽  
John D. McKinney
Keyword(s):  
Cell Division ◽  
Single Cell ◽  
Chromosome Replication ◽  
Cell Dynamics

scholarly journals Stem cell dynamics in mouse hair follicles: A story from cell division counting and single cell lineage tracing

Cell Cycle ◽  
2010 ◽  
Vol 9 (8) ◽  
pp. 1504-1510 ◽  
Author(s):  
Ying V. Zhang ◽  
Brian S. White ◽  
David I. Shalloway ◽  
Tudorita Tumbar
Keyword(s):  
Stem Cell ◽  
Cell Division ◽  
Single Cell ◽  
Cell Lineage ◽  
Hair Follicles ◽  
Lineage Tracing ◽  
Cell Dynamics

scholarly journals Balanced transcription of cell division genes inBacillus subtilisas revealed by single cell analysis

2013 ◽  
Vol 15 (12) ◽  
pp. 3196-3209 ◽  
Author(s):  
Erik Nico Trip ◽  
Jan-Willem Veening ◽  
Eric J. Stewart ◽  
Jeff Errington ◽  
Dirk-Jan Scheffers
Keyword(s):  
Cell Division ◽  
Single Cell ◽  
Single Cell Analysis ◽  
Cell Analysis

Single-cell dynamics of T-cell priming

2007 ◽  
Vol 19 (3) ◽  
pp. 249-258 ◽  
Author(s):  
S HENRICKSON ◽  
U VONANDRIAN
Keyword(s):  
T Cell ◽  
Single Cell ◽  
Cell Dynamics ◽  
T Cell Priming

scholarly journals Extensive Regulation of Metabolism and Growth during the Cell Division Cycle

2014 ◽  
Author(s):  
Nikolai Slavov ◽  
David Botstein ◽  
Amy Caudy
Keyword(s):  
Gene Expression ◽  
Oxygen Consumption ◽  
Cell Division ◽  
Single Cell ◽  
Single Cells ◽  
Emergent Behavior ◽  
Yeast Cells ◽  
Physiological Data ◽  
Synchronized Cultures ◽  
Metabolic Cycle

Yeast cells grown in culture can spontaneously synchronize their respiration, metabolism, gene expression and cell division. Such metabolic oscillations in synchronized cultures reflect single-cell oscillations, but the relationship between the oscillations in single cells and synchronized cultures is poorly understood. To understand this relationship and the coordination between metabolism and cell division, we collected and analyzed DNA-content, gene-expression and physiological data, at hundreds of time-points, from cultures metabolically-synchronized at different growth rates, carbon sources and biomass densities. The data enabled us to extend and generalize our mechanistic model, based on ensemble average over phases (EAP), connecting the population-average gene-expression of asynchronous cultures to the gene-expression dynamics in the single-cells comprising the cultures. The extended model explains the carbon-source specific growth-rate responses of hundreds of genes. Our physiological data demonstrate that the frequency of metabolic cycling in synchronized cultures increases with the biomass density, suggesting that this cycling is an emergent behavior, resulting from the entraining of the single-cell metabolic cycle by a quorum-sensing mechanism, and thus underscoring the difference between metabolic cycling in single cells and in synchronized cultures. Measurements of constant levels of residual glucose across metabolically synchronized cultures indicate that storage carbohydrates are required to fuel not only the G1/S transition of the division cycle but also the metabolic cycle. Despite the large variation in profiled conditions and in the scale of their dynamics, most genes preserve invariant dynamics of coordination with each other and with the rate of oxygen consumption. Similarly, the G1/S transition always occurs at the beginning, middle or end of the high oxygen consumption phases, analogous to observations in human and drosophila cells. These results highlight evolutionary conserved coordination among metabolism, cell growth and division.

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