scholarly journals Proteomic profiling of Myc-associated proteins 

Cell Cycle ◽  
2010 ◽  
Vol 9 (24) ◽  
pp. 4908-4921 ◽  
Author(s):  
Pooja Agrawal ◽  
Kebing Yu ◽  
Arthur R. Salomon ◽  
John M. Sedivy
Keyword(s):  
Proteomic Profiling ◽  
Associated Proteins

Proteomic profiling of cell envelope-associated proteins fromStaphylococcus aureus

PROTEOMICS ◽  
2006 ◽  
Vol 6 (5) ◽  
pp. 1530-1549 ◽  
Author(s):  
Christine L. Gatlin ◽  
Rembert Pieper ◽  
Shih-Ting Huang ◽  
Emmanuel Mongodin ◽  
Elizabeth Gebregeorgis ◽  
...  
Keyword(s):  
Cell Envelope ◽  
Proteomic Profiling ◽  
Associated Proteins

scholarly journals Longitudinal proteomic profiling reveals increased early inflammation and sustained apoptosis proteins in severe COVID-19

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Liis Haljasmägi ◽  
Ahto Salumets ◽  
Anna Pauliina Rumm ◽  
Meeri Jürgenson ◽  
Ekaterina Krassohhina ◽  
...  
Keyword(s):  
Plasma Proteins ◽  
Proteomic Profiling ◽  
Apoptotic Pathway ◽  
Neutralization Capacity ◽  
Inflammatory Conditions ◽  
Associated Proteins ◽  
Life Threatening ◽  
Inflammatory Milieu ◽  
Apoptosis Proteins ◽  
Cardinal Feature

AbstractSARS-CoV-2 infection has a risk to develop into life-threatening COVID-19 disease. Whereas age, hypertension, and chronic inflammatory conditions are risk factors, underlying host factors and markers for disease severity, e.g. requiring intensive care unit (ICU) treatment, remain poorly defined. To this end, we longitudinally profiled blood inflammation markers, antibodies, and 101 plasma proteins of hospitalized COVID-19 patients who did or did not require ICU admission. While essentially all patients displayed SARS-CoV-2-specific antibodies and virus-neutralization capacity within 12–15 days, a rapid, mostly transient upregulation of selective inflammatory markers including IL-6, CXCL10, CXCL11, IFNγ, IL-10, and monocyte-attracting CCL2, CCL7 and CCL8, was particularly evident in ICU patients. In addition, there was consistent and sustained upregulation of apoptosis-associated proteins CASP8, TNFSF14, HGF, and TGFB1, with HGF discriminating between ICU and non-ICU cohorts. Thus, COVID-19 is associated with a selective inflammatory milieu within which the apoptotic pathway is a cardinal feature with potential to aid risk-based patient stratification.

scholarly journals Comparative Proteomic Profiling of Ehrlichia ruminantium Pathogenic Strain and Its High-Passaged Attenuated Strain Reveals Virulence and Attenuation-Associated Proteins

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0145328 ◽  
Author(s):  
Isabel Marcelino ◽  
Miguel Ventosa ◽  
Elisabete Pires ◽  
Markus Müller ◽  
Frédérique Lisacek ◽  
...  
Keyword(s):  
Pathogenic Strain ◽  
Proteomic Profiling ◽  
Ehrlichia Ruminantium ◽  
Associated Proteins

Novel localizations and interactions of intercellular bridge proteins revealed by proteomic profiling†

2020 ◽  
Vol 102 (5) ◽  
pp. 1134-1144 ◽  
Author(s):  
Tokuko Iwamori ◽  
Naoki Iwamori ◽  
Masaki Matsumoto ◽  
Hiroyuki Imai ◽  
Etsuro Ono
Keyword(s):  
Germ Cells ◽  
Small Rnas ◽  
Protein Complexes ◽  
Proteomic Profiling ◽  
Intercellular Bridge ◽  
Proteomics Analysis ◽  
Meiotic Chromosomes ◽  
Junction Protein ◽  
Associated Proteins ◽  
Ectoplasmic Specialization

Abstract Intercellular bridges (ICBs) connecting germ cells are essential for spermatogenesis, and their deletion causes male infertility. However, the functions and component factors of ICBs are still unknown. We previously identified novel ICB-associated proteins by proteomics analysis using ICB enrichment. Here, we performed immunoprecipitation–proteomics analyses using antibodies specific to known ICB proteins MKLP1, RBM44, and ectoplasmic specialization-associated protein KIAA1210 and predicted protein complexes in the ICB cores. KIAA1210, its binding protein topoisomerase2B (TOP2B), and tight junction protein ZO1 were identified as novel ICB proteins. On the other hand, as well as KIAA1210 and TOP2B, MKLP1 and RBM44, but not TEX14, were localized at the XY body of spermatocytes, suggesting that there is a relationship between ICB proteins and meiotic chromosomes. Moreover, small RNAs interacted with an ICB protein complex that included KIAA1210, RBM44, and MKLP1. These results indicate dynamic movements of ICB proteins and suggest that ICB proteins could be involved not only in the communication between germ cells but also in their epigenetic regulation. Our results provide a novel perspective on the function of ICBs and could be helpful in revealing the biological function of the ICB.

scholarly journals Multivariate proteomic profiling identifies novel accessory proteins of coated vesicles

2012 ◽  
Vol 197 (1) ◽  
pp. 141-160 ◽  
Author(s):  
Georg H.H. Borner ◽  
Robin Antrobus ◽  
Jennifer Hirst ◽  
Gary S. Bhumbra ◽  
Patrycja Kozik ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Isotope Labeling ◽  
Principal Component ◽  
Coated Vesicles ◽  
Coated Vesicle ◽  
Coat Proteins ◽  
Proteomic Profiling ◽  
Transport Vesicles ◽  
Associated Proteins ◽  
Sirna Knockdown

Despite recent advances in mass spectrometry, proteomic characterization of transport vesicles remains challenging. Here, we describe a multivariate proteomics approach to analyzing clathrin-coated vesicles (CCVs) from HeLa cells. siRNA knockdown of coat components and different fractionation protocols were used to obtain modified coated vesicle-enriched fractions, which were compared by stable isotope labeling of amino acids in cell culture (SILAC)-based quantitative mass spectrometry. 10 datasets were combined through principal component analysis into a “profiling” cluster analysis. Overall, 136 CCV-associated proteins were predicted, including 36 new proteins. The method identified >93% of established CCV coat proteins and assigned >91% correctly to intracellular or endocytic CCVs. Furthermore, the profiling analysis extends to less well characterized types of coated vesicles, and we identify and characterize the first AP-4 accessory protein, which we have named tepsin. Finally, our data explain how sequestration of TACC3 in cytosolic clathrin cages causes the severe mitotic defects observed in auxilin-depleted cells. The profiling approach can be adapted to address related cell and systems biological questions.

scholarly journals Chromatin proteomic profiling reveals novel proteins associated with histone-marked genomic regions

2015 ◽  
Vol 112 (12) ◽  
pp. 3841-3846 ◽  
Author(s):  
Xiong Ji ◽  
Daniel B. Dadon ◽  
Brian J. Abraham ◽  
Tong Ihn Lee ◽  
Rudolf Jaenisch ◽  
...  
Keyword(s):  
Es Cells ◽  
Cell Types ◽  
The Novel ◽  
Proteomic Profiling ◽  
Novel Proteins ◽  
Disease Mechanisms ◽  
Lysine Residues ◽  
Associated Proteins ◽  
And Function ◽  
Genomic Regions

More than a thousand proteins are thought to contribute to mammalian chromatin and its regulation, but our understanding of the genomic occupancy and function of most of these proteins is limited. Here we describe an approach, which we call “chromatin proteomic profiling,” to identify proteins associated with genomic regions marked by specifically modified histones. We used ChIP-MS to identify proteins associated with genomic regions marked by histones modified at specific lysine residues, including H3K27ac, H3K4me3, H3K79me2, H3K36me3, H3K9me3, and H4K20me3, in ES cells. We identified 332 known and 114 novel proteins associated with these histone-marked genomic segments. Many of the novel candidates have been implicated in various diseases, and their chromatin association may provide clues to disease mechanisms. More than 100 histone modifications have been described, so similar chromatin proteomic profiling studies should prove to be valuable for identifying many additional chromatin-associated proteins in a broad spectrum of cell types.

Proteomic profiling of intestinal prechylomicron transport vesicle (PCTV)-associated proteins in an animal model of insulin resistance (94 char)

2010 ◽  
Vol 73 (7) ◽  
pp. 1291-1305 ◽  
Author(s):  
Diana M. Wong ◽  
Jennifer P. Webb ◽  
Paul M. Malinowski ◽  
Elaine Xu ◽  
Joseph Macri ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Animal Model ◽  
Proteomic Profiling ◽  
Transport Vesicle ◽  
Associated Proteins
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