scholarly journals Emerging functions of c-kit and its ligand stem cell factor in dendritic cells

Cell Cycle ◽  
2008 ◽  
Vol 7 (18) ◽  
pp. 2826-2832 ◽  
Author(s):  
Prabir Ray ◽  
Nandini Krishnamoorthy ◽  
Anuradha Ray
Keyword(s):  
Dendritic Cells ◽  
Stem Cell ◽  
Stem Cell Factor

scholarly journals Cutting Edge: MMP-9 Inhibits IL-23p19 Expression in Dendritic Cells by Targeting Membrane Stem Cell Factor Affecting Lung IL-17 Response

2014 ◽  
Vol 192 (12) ◽  
pp. 5471-5475 ◽  
Author(s):  
Timothy B. Oriss ◽  
Nandini Krishnamoorthy ◽  
Mahesh Raundhal ◽  
Christina Morse ◽  
Krishnendu Chakraborty ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Stem Cell ◽  
Stem Cell Factor ◽  
Cutting Edge

Generation of functionally mature dendritic cells from the multipotential stem cell line FDCP-mix

2000 ◽  
Vol 111 (3) ◽  
pp. 890-897 ◽  
Author(s):  
Timm Schroeder ◽  
Claudia Lange ◽  
John Strehl ◽  
Ursula Just
Keyword(s):  
Dendritic Cells ◽  
Stem Cell ◽  
Cell Line ◽  
Stem Cell Line

Stem Cell Factor

2002 ◽  
Vol 14 (2) ◽  
pp. 0052-0059 ◽  
Author(s):  
Susan Smith ◽  
Adrian Piliponsky ◽  
Mor-Li Hartman ◽  
Francesca Levi-Schaffer
Keyword(s):  
Stem Cell ◽  
Stem Cell Factor

Downregulation of c-kit (Stem Cell Factor Receptor) in Transformed Hematopoietic Precursor Cells by Stroma Cells

Blood ◽  
1999 ◽  
Vol 93 (2) ◽  
pp. 554-563 ◽  
Author(s):  
Christoph Heberlein ◽  
Jutta Friel ◽  
Christine Laker ◽  
Dorothee von Laer ◽  
Ulla Bergholz ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Line ◽  
Stem Cell Factor ◽  
Progenitor Cell ◽  
Receptor Expression ◽  
General Mechanism ◽  
Ligand Interaction ◽  
Kit Ligand ◽  
Kit Receptor ◽  
Progenitor Cell Line

Abstract We show a dramatic downregulation of the stem cell factor (SCF) receptor in different hematopoietic cell lines by murine stroma. Growth of the human erythroid/macrophage progenitor cell line TF-1 is dependent on granulocyte-macrophage colony-stimulating factor (GM-CSF) or interleukin-3 (IL-3). However, TF-1 cells clone and proliferate equally well on stroma. Independent stroma-dependent TF-1 clones (TF-1S) were generated on MS-5 stroma. Growth of TF-1S and TF-1 cells on stroma still requires interaction between c-kit (SCF receptor) and its ligand SCF, because antibodies against c-kit inhibit growth to less than 2%. Surprisingly, c-kit receptor expression (RNA and protein) was downregulated by 2 to 3 orders of magnitude in TF-1S and TF-1 cells grown on stroma. This stroma-dependent regulation of the kit receptor in TF-1 was also observed on exposure to kit ligand-negative stroma, thus indicating the need for heterologous receptor ligand interaction. Removal of stroma induced upregulation by 2 to 4 orders of magnitude. Downregulation and upregulation of c-kit expression could also be shown for the megakaryocytic progenitor cell line M-07e and was comparable to that of TF-1, indicating that stroma-dependent regulation of c-kit is a general mechanism. Downregulation may be an economic way to compensate for the increased sensitivity of the c-kit/ligand interaction on stroma. The stroma-dependent c-kit regulation most likely occurs at the transcriptional level, because mechanisms, such as splicing, attenuation, differential promoter usage, or mRNA stability, could be excluded.

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