Cord Blood CD34+Cells Cultured with FLT3L, Stem Cell Factor, Interleukin-6, and IL-3 Produce CD11c+CD1a−/c−Myeloid Dendritic Cells

2007 ◽  
Vol 16 (5) ◽  
pp. 849-856 ◽  
Author(s):  
S.A.W. Fadilah ◽  
S. Vuckovic ◽  
D. Khalil ◽  
D.N.J. Hart
Keyword(s):  
Dendritic Cells ◽  
Stem Cell ◽  
Cord Blood ◽  
Interleukin 6 ◽  
Stem Cell Factor ◽  
Myeloid Dendritic Cells ◽  
Cd34 Cells ◽  
Cord Blood Cd34 ◽  
Cells Cultured

scholarly journals Residues 39-56 of Stem Cell Factor Protein Sequence Are Capable of Stimulating the Expansion of Cord Blood CD34+ Cells

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0141485 ◽  
Author(s):  
Bin Shen ◽  
Wenhong Jiang ◽  
Jie Fan ◽  
Wei Dai ◽  
Xinxin Ding ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cord Blood ◽  
Protein Sequence ◽  
Stem Cell Factor ◽  
Cd34 Cells ◽  
Cord Blood Cd34

Decrease in Apoptosis and Increase in Polyploidization of Megakaryocytes by Stem Cell Factor During Ex Vivo Expansion of Human Cord Blood CD34+Cells Using Thrombopoietin

Stem Cells ◽  
2002 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
Jeong-Hae Kie ◽  
Woo-Ick Yang ◽  
Mi-Kyung Lee ◽  
Tae-Jung Kwon ◽  
Yoo-Hong Min ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cord Blood ◽  
Stem Cell Factor ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Human Cord Blood ◽  
Cd34 Cells ◽  
Cord Blood Cd34

scholarly journals Stem cell factor is essential for preserving NOD/SCID reconstitution capacity ofex vivoexpanded cord blood CD34+cells

2015 ◽  
Vol 48 (3) ◽  
pp. 293-300 ◽  
Author(s):  
Zheng Du ◽  
Ziyan Wang ◽  
Weiwei Zhang ◽  
Haibo Cai ◽  
Wen-Song Tan
Keyword(s):  
Stem Cell ◽  
Cord Blood ◽  
Stem Cell Factor ◽  
Cd34 Cells ◽  
Cord Blood Cd34

Clinical-Scale Cultures of Cord Blood CD34+Cells to Amplify Committed Progenitors and Maintain Stem Cell Activity

2011 ◽  
Vol 20 (9) ◽  
pp. 1453-1464 ◽  
Author(s):  
Zoran Ivanovic ◽  
Pascale Duchez ◽  
Jean Chevaleyre ◽  
Marija Vlaski ◽  
Xavier Lafarge ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cord Blood ◽  
Cell Activity ◽  
Clinical Scale ◽  
Cd34 Cells ◽  
Stem Cell Activity ◽  
Cord Blood Cd34

Recombinant Tpo Stimulates Maturation of Megakaryocytes Derived from Adult Peripheral Blood- but Not Cord Blood-CD34+ Cells.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 1191-1191
Author(s):  
Karen M. Pastos ◽  
William B. Slayton ◽  
Lisa M. Rimsza ◽  
Martha C. Sola
Keyword(s):  
Cord Blood ◽  
Peripheral Blood ◽  
Developmental Differences ◽  
Ploidy Levels ◽  
Cd34 Cells ◽  
Cord Blood Cd34 ◽  
Adult Bone ◽  
Adult Peripheral Blood ◽  
Cells Cultured

Abstract Umbilical cord blood (CB) is a valuable source of stem cells for transplantation. However, platelet engraftment is slow, taking approximately 70 days for CB transplants versus 20 days for mobilized adult peripheral blood (PB) transplants. This time is not significantly shortened by the administration of recombinant thrombopoietin (rTpo). The cause for the delay in platelet engraftment following CB transplant is unknown. We hypothesized that developmental differences in size and ploidy of neonatal versus adult megakaryocytes (MKs) contribute to this delay. To mimic these two types of transplant in vitro, we compared CB to PB CD34+ cells cultured in adult bone marrow stromal-conditioned media (CM) or unconditioned media (UCM) for 14 days. Increasing doses of rTpo were added to the CM, and the resulting MK maturation was compared with that of UCM with maximal rTpo concentration. MK number and ploidy were determined by flow cytometry using CD41-FITC and propidium iodide, respectively. Increased ploidy levels were expressed as percentage of MKs with a ploidy ≥ 8N. Results represent an average of three independent experiments. Figure Figure As seen in the figure, PB-derived MKs reached highest ploidy levels in the presence of UCM + 100 ng/ml rTpo. When cultured in CM, they exhibited lower ploidy levels, regardless of Tpo concentration. In contrast, CB-derived MKs exhibited higher ploidy levels in response to CM with either 0 or 0.1 ng/ml (physiologic concentration) of rTpo, as compared to higher rTpo concentrations or UCM + 100 ng/ml rTpo. MK numbers increased in response to rTpo in a dose-response manner, regardless of the source of the MKs (data not shown). These results indicate that intrinsic differences between CB- and PB-derived megakaryocytes exist, and that maturation is regulated differently in neonatal versus adult MKs. While Tpo is a potent stimulator of MK maturation in PB-derived MKs, it appears to inhibit this process in CB-derived MKs. These differences may be relevant to understanding the delayed platelet engraftment following CB transplants.

CD13/N-aminopeptidase is involved in the development of dendritic cells and macrophages from cord blood CD34+ cells

Blood ◽  
2000 ◽  
Vol 95 (2) ◽  
pp. 453-460 ◽  
Author(s):  
Michelle Rosenzwajg ◽  
Ludovic Tailleux ◽  
Jean Claude Gluckman
Keyword(s):  
Dendritic Cells ◽  
Cell Growth ◽  
Cord Blood ◽  
Cell Activation ◽  
Aminopeptidase Activity ◽  
Cd34 Cells ◽  
M Phase ◽  
Cord Blood Cd34 ◽  
Macrophage Colony ◽  
Blocking Antibodies

Expression of CD13/N-aminopeptidase may reflect cell activation and growth. We examined its role regarding cell growth in cultures of cord blood CD34+ cells with stem cell factor/Flt-3 ligand/granulocyte-macrophage colony-stimulating factor/tumor necrosis factor-. Indeed, 82% ± 6% of cells from culture day 5 were CD13hi, 25% ± 8% of which were still Lin−. About 50% of CD13hiLin− cells, which comprise progenitors of dendritic cells (DC), monocytes/macrophages and granulocytes, and 30% of CD13loLin− cells were CD34+. Sorted CD34+CD13hiLin− cells, cultured further for 7 days with the same cytokines, expanded 31-fold and CD34-CD13hiLin− cells 7-fold, but CD34+CD13loLin− and CD34−CD13loLin− cells did not grow. Thus, cell growth correlated with CD13 expression, all the more so that cells were CD34+. Actinonin, the most potent N-aminopeptidase inhibitor, was used to engage CD13 on sorted CD13hiLin− cells and on culture day-7 bulk cells. In both cases, this resulted in reversible cell growth arrest, with 30% to 60% fewer cells in the G2/S-M phase than in controls. Interestingly, similar effects were noted with CD13 monoclonal antibody TÜK1, which does not inhibit N-aminopeptidase activity, but not with N-aminopeptidase-blocking antibodies WM15 and F23. All cycling cells appeared susceptible to actinonin, which induced cell apoptosis at the same time as Bcl-2 was downregulated and caspase-3 activity increased, but finally percentages and yields of DC and macrophage precursors were affected more than those of granulocytic cells. Thus, through engagement of N-aminopeptidase enzymatic site but possibly also of an independent determinant, CD13 plays a role in the growth of DC/macrophage progenitors and precursors.

Interleukin-6 Directly Modulates Stem Cell Factor-Dependent Development of Human Mast Cells Derived From CD34+Cord Blood Cells

Blood ◽  
1999 ◽  
Vol 94 (2) ◽  
pp. 496-508 ◽  
Author(s):  
Tatsuya Kinoshita ◽  
Nobukuni Sawai ◽  
Eiko Hidaka ◽  
Tetsuji Yamashita ◽  
Kenichi Koike
Keyword(s):  
Mast Cell ◽  
Stem Cell ◽  
Mast Cells ◽  
Cord Blood ◽  
Interleukin 6 ◽  
Blood Cells ◽  
Stem Cell Factor ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Cord Blood Cells

In the present study, we attempted to clarify the effects of interleukin-6 (IL-6) on the growth and properties of human mast cells using cultured mast cells selectively generated by stem cell factor (SCF) from CD34+ cord blood cells. The addition of IL-6 to cultures containing mast cells resulted in a substantial reduction of the number of progenies grown by SCF in the liquid culture. This IL-6–mediated inhibition of mast cell growth may be due in part to the suppression at the precursor level, according to the results of a clonal cell culture assay. Moreover, a flow cytometric analysis showed that the cultured mast cells grown in the presence of SCF+IL-6 had decreased c-kit expression. The exposure of cultured mast cells to SCF+IL-6 also caused substantial increases in the cell size, frequency of chymase-positive cells, and intracellular histamine level compared with the values obtained with SCF alone. The flow cytometric analysis showed low but significant levels of expression of IL-6 receptor (IL-6R) and gp130 on the cultured mast cells grown with SCF. The addition of either anti–IL-6R antibody or anti-gp130 antibody abrogated the biological functions of IL-6. Although IL-4 exerted an effect similar to that of IL-6 on the cultured mast cells under stimulation with SCF, the results of comparative experiments suggest that the two cytokines use different regulatory mechanisms. Taken together, the present findings suggest that IL-6 modulates SCF-dependent human mast cell development directly via an IL-6R-gp130 system.

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