scholarly journals Caveolin-1-Deficient Mice Have An Increased Mammary Stem Cell Population with Upregulation of Wnt/?-Catenin Signaling

Cell Cycle ◽  
2005 ◽  
Vol 4 (12) ◽  
pp. 1808-1816 ◽  
Author(s):  
Federica Sotgia ◽  
Terence M. Williams ◽  
Alex W. Cohen ◽  
Carlo Minetti ◽  
Richard G. Pestell ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Population ◽  
Mammary Stem Cell ◽  
Stem Cell Population ◽  
Caveolin 1 ◽  
Mammary Stem ◽  
Deficient Mice

scholarly journals 3D Differentiation Of Mammosphere Derived Macaca fascicularis’s Mammary Stem Cells

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Silmi Mariya
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Mammary Gland ◽  
Cell Population ◽  
Mammary Stem Cell ◽  
Stem Cell Population ◽  
Stem Cell Markers ◽  
Mammary Stem Cells ◽  
Surgical Biopsy ◽  
Mammary Stem

The mammary gland contains adult stem cells that are capable of self-renewal.  This population plays an important role in the development of mammary gland and breast cancer pathogenesis. The studies of mammary stem cells are limited due to the difficulty to acquire and expand adult stem cell population in an undifferentiated state. In this study, we developed mammosphere cultures of nulliparous cynomolgus monkeys (Macaca fascicularis; Mf) as a culture system to enrich mammary stem cells. This species has similarity of mammary gland structure as humans including anatomy, developmental stages, and lobule profile of mammary gland. The use of stem cells from primate animals is essential to bridge the knowledge gaps resulting from stem cell research using rodents for clinical trials in human. Small samples of mammary tissues were collected by surgical biopsy; cells were cultured as monolayer and cryopreserved. Cryopreserved cells were cultured into mammospheres, and the expression of markers for mammary stem cells was evaluated using qPCR. Cells were further differentiated with 3D approaches to evaluate morphology and organoid budding. The study showed that mammosphere culture resulted in an increase in the expression of mammary stem cell markers with each passage. The 3D differentiation in matrigel allowed for organoid formation. Mammary gland stem cells have been successfully differentiated which characterized by CSN2 marker expression and differentiation regulators marker STAT5 and GATA3. The results indicate that mammospheres can be successfully developed derived from breast tissue of nulliparous Mf collected via surgical biopsy. As the mammosphere allows for enrichment of mammary stem cell population, the findings also suggest that a 3-dimensional system is efficient as in-vitro model to study mammary stem cells and a useful system to study mammary differentiation in regards to cancer prevention.

scholarly journals A Mammary Stem Cell Population Identified and Characterized in Late Embryogenesis Reveals Similarities to Human Breast Cancer

2012 ◽  
Vol 10 (2) ◽  
pp. 183-197 ◽  
Author(s):  
Benjamin T. Spike ◽  
Dannielle D. Engle ◽  
Jennifer C. Lin ◽  
Samantha K. Cheung ◽  
Justin La ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cell ◽  
Cell Population ◽  
Human Breast Cancer ◽  
Mammary Stem Cell ◽  
Stem Cell Population ◽  
Human Breast ◽  
Late Embryogenesis ◽  
Mammary Stem

CD24 and CD49f expressions of E14.5 mouse mammary anlagen cells define putative distribution of earlier embryonic mammary stem cell activities

2018 ◽  
Vol 96 (5) ◽  
pp. 539-547 ◽  
Author(s):  
Jiazhe Song ◽  
Fangrong Ding ◽  
Song Li ◽  
Wenzhe Li ◽  
Ning Li ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mammary Gland ◽  
Cell Population ◽  
Cell Biology ◽  
Cell Activity ◽  
Mammary Stem Cell ◽  
Flow Cytometry Analysis ◽  
Large Numbers ◽  
Stem Cell Activity ◽  
Mammary Stem

Stem cell biology offers promise for understanding the origins of the mammary gland. However, the distribution of mammary stem cell (MaSC) activities at earlier embryonic stages has not been fully identified. The markers for sorting adult MaSC, CD24, CD29, and CD49f have been applied to analyze fetal MaSCs. Here we explored mammary anlagen MaSCs by investigating the expression of CD24 and CD49f. According to the comparative analysis between adult mammary gland and fetal mammary anlagen, we found that fetal mouse mammary anlagen may possess a high percentage of potential MaSCs. Flow cytometry analysis revealed 2 distinct mammary anlagen populations: Lin–CD24med and Lin–CD24high. Sphere-forming and mammary repopulating assays confirmed that the stem cell activity of E14.5 mouse mammary anlagen was restricted to the Lin–CD24med cell population. Furthermore, CD24med mammary anlagen cells were separated into Lin–CD24medCD49f+ and Lin–CD24medCD49f– populations and identified, respectively. The results proved that the mammary anlagen Lin–CD24medCD49f+ cell population possesses more stem cell activities than the Lin–CD24medCD49f– cell population. However, a limited numbers of stem cells and large numbers of stromal cells were identified in mammary anlagen in the Lin–CD24med cell population.

Stem cells in mammary health and milk production

2021 ◽  
Vol 16 ◽  
Author(s):  
Ratan K Choudhary ◽  
Fenq-Qi Zhao
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cell Population ◽  
Adult Stem Cells ◽  
Stem Cell Population ◽  
Mammary Stem ◽  
In Vitro Expansion

: Adult stem cells like mammary and mesenchymal stem cells have received significant attention because these stem cells (SCs) possess therapeutic potential in treating many animal diseases. These cells can be administered in an autologous or allogenic fashion, either freshly isolated from the donor tissue or previously cultured and expanded in vitro. Expansion of adult stem cells is a prerequisite before therapeutic application because sufficient numbers are required in dosage calculation. Stem cells directly and indirectly (by secreting various growth factors and angiogenic factors called secretome) act to repair and regenerate injured tissues. Recent studies on mammary stem cells showed in vivo and in vitro expansion ability by removing the blockage of asymmetrical cell division. Compounds like purine analogs (xanthosine, xanthine, and inosine) or hormones (progesterone and bST) help increase stem cell population by promoting cell division. Such methodology of enhancing stem cells number, either in vivo or in vitro, may help in preclinical studies for translational research like treating diseases like mastitis. The application of mesenchymal stem cells has also been shown to benefit mammary gland health due to the ‘homing’ property of stem cells. In addition to that, the multiple positive effects of stem cell secretome are on mammary tissue healing and killing bacteria is novel in the production of quality milk. This systematic review discusses some of the studies on stem cells that have been useful in increasing the stem cell population and increasing mammary stem/progenitor cells. Finally, we provide insights into how enhancing mammary stem cell population could potentially increase terminally differentiated cells, ultimately leading to more milk production.

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