scholarly journals A Mammary Stem Cell Population Identified and Characterized in Late Embryogenesis Reveals Similarities to Human Breast Cancer

2012 ◽  
Vol 10 (2) ◽  
pp. 183-197 ◽  
Author(s):  
Benjamin T. Spike ◽  
Dannielle D. Engle ◽  
Jennifer C. Lin ◽  
Samantha K. Cheung ◽  
Justin La ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cell ◽  
Cell Population ◽  
Human Breast Cancer ◽  
Mammary Stem Cell ◽  
Stem Cell Population ◽  
Human Breast ◽  
Late Embryogenesis ◽  
Mammary Stem

scholarly journals 3D Differentiation Of Mammosphere Derived Macaca fascicularis’s Mammary Stem Cells

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Silmi Mariya
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Mammary Gland ◽  
Cell Population ◽  
Mammary Stem Cell ◽  
Stem Cell Population ◽  
Stem Cell Markers ◽  
Mammary Stem Cells ◽  
Surgical Biopsy ◽  
Mammary Stem

The mammary gland contains adult stem cells that are capable of self-renewal.  This population plays an important role in the development of mammary gland and breast cancer pathogenesis. The studies of mammary stem cells are limited due to the difficulty to acquire and expand adult stem cell population in an undifferentiated state. In this study, we developed mammosphere cultures of nulliparous cynomolgus monkeys (Macaca fascicularis; Mf) as a culture system to enrich mammary stem cells. This species has similarity of mammary gland structure as humans including anatomy, developmental stages, and lobule profile of mammary gland. The use of stem cells from primate animals is essential to bridge the knowledge gaps resulting from stem cell research using rodents for clinical trials in human. Small samples of mammary tissues were collected by surgical biopsy; cells were cultured as monolayer and cryopreserved. Cryopreserved cells were cultured into mammospheres, and the expression of markers for mammary stem cells was evaluated using qPCR. Cells were further differentiated with 3D approaches to evaluate morphology and organoid budding. The study showed that mammosphere culture resulted in an increase in the expression of mammary stem cell markers with each passage. The 3D differentiation in matrigel allowed for organoid formation. Mammary gland stem cells have been successfully differentiated which characterized by CSN2 marker expression and differentiation regulators marker STAT5 and GATA3. The results indicate that mammospheres can be successfully developed derived from breast tissue of nulliparous Mf collected via surgical biopsy. As the mammosphere allows for enrichment of mammary stem cell population, the findings also suggest that a 3-dimensional system is efficient as in-vitro model to study mammary stem cells and a useful system to study mammary differentiation in regards to cancer prevention.

scholarly journals Caveolin-1-Deficient Mice Have An Increased Mammary Stem Cell Population with Upregulation of Wnt/?-Catenin Signaling

Cell Cycle ◽  
2005 ◽  
Vol 4 (12) ◽  
pp. 1808-1816 ◽  
Author(s):  
Federica Sotgia ◽  
Terence M. Williams ◽  
Alex W. Cohen ◽  
Carlo Minetti ◽  
Richard G. Pestell ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Population ◽  
Mammary Stem Cell ◽  
Stem Cell Population ◽  
Caveolin 1 ◽  
Mammary Stem ◽  
Deficient Mice

Adipose-derived stem cells enhance human breast cancer growth and cancer stem cell-like properties through adipsin

Oncogene ◽  
2018 ◽  
Vol 38 (6) ◽  
pp. 767-779 ◽  
Author(s):  
Hideaki Goto ◽  
Yohei Shimono ◽  
Yohei Funakoshi ◽  
Yoshinori Imamura ◽  
Masanori Toyoda ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Human Breast Cancer ◽  
Cancer Growth ◽  
Adipose Derived Stem Cells ◽  
Human Breast ◽  
Breast Cancer Growth

scholarly journals Multiple Lineages of Human Breast Cancer Stem/Progenitor Cells Identified by Profiling with Stem Cell Markers

PLoS ONE ◽  
2009 ◽  
Vol 4 (12) ◽  
pp. e8377 ◽  
Author(s):  
Wendy W. Hwang-Verslues ◽  
Wen-Hung Kuo ◽  
Po-Hao Chang ◽  
Chi-Chun Pan ◽  
Hsing-Hui Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cell ◽  
Progenitor Cells ◽  
Human Breast Cancer ◽  
Stem Cell Markers ◽  
Human Breast ◽  
Cell Markers
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