scholarly journals Escherichia coliNissle 1917-derived factors reduce cell death and late apoptosis and increase transepithelial electrical resistance in a model of 5-fluorouracil-induced intestinal epithelial cell damage

2014 ◽  
Vol 15 (5) ◽  
pp. 560-569 ◽  
Author(s):  
Hanru Wang ◽  
Susan EP Bastian ◽  
Ker Y Cheah ◽  
Andrew Lawrence ◽  
Gordon S Howarth
Keyword(s):  
Cell Death ◽  
Epithelial Cell ◽  
Electrical Resistance ◽  
Intestinal Epithelial Cell ◽  
Cell Damage ◽  
Transepithelial Electrical Resistance ◽  
Intestinal Epithelial ◽  
Late Apoptosis

Mechanisms of Bile Acid-Induced Intestinal Epithelial Cell Death

2014 ◽  
Vol 219 (3) ◽  
pp. S14-S15
Author(s):  
Anne S. Roberts ◽  
Stephanie C. Papillon ◽  
Avafia Y. Roberts ◽  
Mark R. Frey ◽  
Henri R. Ford ◽  
...  
Keyword(s):  
Cell Death ◽  
Bile Acid ◽  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Epithelial Cell Death

scholarly journals Different Sources of Copper Effect on Intestinal Epithelial Cell: Toxicity, Oxidative Stress, and Metabolism

Metabolites ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 11
Author(s):  
Runxian Li ◽  
Yang Wen ◽  
Gang Lin ◽  
Chengzhen Meng ◽  
Pingli He ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Epithelial Cell ◽  
Protein Expression ◽  
Intestinal Epithelial Cell ◽  
Cell Damage ◽  
Intestinal Epithelial ◽  
Protein Expression Level ◽  
Treatment Groups ◽  
All Treatment

Copper (Cu) is widely used in the swine industry to improve the growth performance of pigs. However, high doses of copper will induce cell damage and toxicity. The aim of this study was to evaluate toxicity, bioavailability, and effects on metabolic processes of varying copper sources using porcine intestinal epithelial cells (IPEC-J2) as a model. The IPEC-J2 were treated with two doses (30 and 120 μM) of CuSO4, Cu Glycine (Cu-Gly), and Cu proteinate (Cu-Pro) for 10 h, respectively. Cell damage and cellular copper metabolism were measured by the changes in cell viability, copper uptake, oxidative stress biomarkers, and gene/protein expression levels. The results showed that cell viability and ratio of reduced and oxidized glutathione (GSH/GSSG) decreased significantly in all treatment groups; intracellular copper content increased significantly in all treatment groups; total superoxide dismutase (SOD) activity increased significantly in the 120 μM exposed groups; SOD1 protein expression levels were significantly upregulated in 30 μM Cu-Pro, 120 μM Cu-Gly, and 120 μM Cu-Pro treatment groups; intracellular reactive oxygen species (ROS) generation and malondialdehyde (MDA) content increased significantly in 30 μM treatment groups and 120 μM CuSO4 treatment group. CTR1 and ATP7A gene expression were significantly downregulated in the 120 μM exposed groups. While upregulation of ATOX1 expression was observed in the presence of 120 μM Cu-Gly and Cu-Pro. ASCT2 gene expression was significantly upregulated after 120 μM Cu-Glycine and CuSO4 exposure, and PepT1 gene expression was significantly upregulated after Cu-Pro exposure. In addition, CTR1 protein expression level decreased after 120 μM CuSO4 and Cu-Gly exposure. PepT1 protein expression level was only upregulated after 120 μM Cu-Pro exposure. These findings indicated that extra copper supplementation can induce intestinal epithelial cell injury, and different forms of copper may have differing effects on cell metabolism.

scholarly journals The Protective Effect of Interleukin-11 on the Cell Death Induced by X-ray Irradiation in Cultured Intestinal Epithelial Cell

2007 ◽  
Vol 48 (2) ◽  
pp. 171-177 ◽  
Author(s):  
Takashi UEMURA ◽  
Toshiyuki NAKAYAMA ◽  
Takafumi KUSABA ◽  
Yuichi YAKATA ◽  
Kazuyuki YAMAZUMI ◽  
...  
Keyword(s):  
Cell Death ◽  
Epithelial Cell ◽  
Protective Effect ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
X Ray ◽  
Interleukin 11

scholarly journals Total Intermittent Pringle Maneuver during Liver Resection Can Induce Intestinal Epithelial Cell Damage and Endotoxemia

PLoS ONE ◽  
2012 ◽  
Vol 7 (1) ◽  
pp. e30539 ◽  
Author(s):  
Simon A. W. G. Dello ◽  
Kostan W. Reisinger ◽  
Ronald M. van Dam ◽  
Marc H. A. Bemelmans ◽  
Toin H. van Kuppevelt ◽  
...  
Keyword(s):  
Liver Resection ◽  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Cell Damage ◽  
Pringle Maneuver ◽  
Intestinal Epithelial

scholarly journals Tauroursodeoxycholic acid inhibits experimental colitis by preventing early intestinal epithelial cell death

2014 ◽  
Vol 94 (12) ◽  
pp. 1419-1430 ◽  
Author(s):  
Debby Laukens ◽  
Lindsey Devisscher ◽  
Lien Van den Bossche ◽  
Pieter Hindryckx ◽  
Roosmarijn E Vandenbroucke ◽  
...  
Keyword(s):  
Cell Death ◽  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Experimental Colitis ◽  
Intestinal Epithelial ◽  
Tauroursodeoxycholic Acid ◽  
Epithelial Cell Death

scholarly journals Application of Measurements of Transepithelial Electrical Resistance of Intestinal Epithelial Cell Monolayers To Evaluate Probiotic Activity

2005 ◽  
Vol 71 (11) ◽  
pp. 7528-7530 ◽  
Author(s):  
Trine Danø Klingberg ◽  
Maja Herold Pedersen ◽  
Avrelija Cencic ◽  
Birgitte Bjørn Budde
Keyword(s):  
Electrical Resistance ◽  
Intestinal Epithelial Cell ◽  
Transepithelial Electrical Resistance ◽  
Intestinal Epithelial ◽  
Lactobacillus Salivarius ◽  
Cell Monolayers ◽  
Epithelial Cell Monolayers ◽  
Probiotic Lactobacilli ◽  
Dose Dependent ◽  
Probiotic Activity

ABSTRACT Among five potentially probiotic lactobacilli investigated, Lactobacillus plantarum MF1298 and Lactobacillus salivarius DC5 showed the highest increase in the transepithelial electrical resistance (TER) of polarized monolayers of Caco-2 cells, and this increase was shown to be dose dependent. Furthermore, preincubation with MF1298 attenuated a decrease in TER induced by Listeria monocytogenes.

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