The role of high-throughput mini-bioreactors in process development and process optimization for mammalian cell culture

2015 ◽  
Vol 3 (6) ◽  
pp. 397-410 ◽  
Author(s):  
Yao-ming Huang ◽  
Chris Kwiatkowski
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Process Optimization ◽  
High Throughput ◽  
Process Development ◽  
Mammalian Cell Culture

Miniature Bioreactors for Rapid Bioprocess Development of Mammalian Cell Culture

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Mohd Helmi Sani ◽  
Frank Baganz
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Process Development ◽  
Mammalian Cell Culture ◽  
Small Scale ◽  
Cell Culture Process ◽  
Deep Wells ◽  
Working Volume ◽  
Culture Process ◽  
And Control

At present, there are a number of commercial small scale shaken systems available on the market with instrumented controllable microbioreactors such as Micro–24 Microreactor System (Pall Corporation, Port Washington, NY) and M2P Biolector, (M2P Labs GmbH, Aachen, Germany). The Micro–24 system is basically an orbital shaken 24–well plate that operates at working volume 3 – 7 mL with 24 independent reactors (deep wells, shaken and sparged) running simultaneously. Each reactor is designed as single use reactor that has the ability to continuously monitor and control the pH, DO and temperature. The reactor aeration is supplied by sparging air from gas feeds that can be controlled individually. Furthermore, pH can be controlled by gas sparging using either dilute ammonia or carbon dioxide directly into the culture medium through a membrane at the bottom of each reactor. Chen et al., (2009) evaluated the Micro–24 system for the mammalian cell culture process development and found the Micro–24 system is suitable as scaledown tool for cell culture application. The result showed that intra-well reproducibility, cell growth, metabolites profiles and protein titres were scalable with 2 L bioreactors.

scholarly journals Criteria for bioreactor comparison and operation standardisation during process development for mammalian cell culture

2011 ◽  
Vol 5 (Suppl 8) ◽  
pp. P47 ◽  
Author(s):  
Oscar Platas Barradas ◽  
Uwe Jandt ◽  
Linh Da Minh Phan ◽  
Mario Villanueva ◽  
Alexander Rath ◽  
...  
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Process Development ◽  
Mammalian Cell Culture

A novel high aspect ratio microfluidic design to provide a stable and uniform microenvironment for cell growth in a high throughput mammalian cell culture array

Lab on a Chip ◽  
2005 ◽  
Vol 5 (1) ◽  
pp. 44 ◽  
Author(s):  
Paul J. Hung ◽  
Philip J. Lee ◽  
Poorya Sabounchi ◽  
Nima Aghdam ◽  
Robert Lin ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Aspect Ratio ◽  
Mammalian Cell ◽  
High Throughput ◽  
High Aspect Ratio ◽  
Mammalian Cell Culture

scholarly journals Protein clustering for cell polarity: Par-3 as a paradigm

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1620 ◽  
Author(s):  
Tony J. C. Harris
Keyword(s):  
Cell Culture ◽  
Caenorhabditis Elegans ◽  
Cell Polarity ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Scaffold Protein ◽  
Opposite Pole ◽  
Biochemical Studies ◽  
Cytoskeletal Networks

The scaffold protein Par-3 (Drosophila Bazooka) is a central organizer of cell polarity across animals. This review focuses on how the clustering of Par-3 contributes to cell polarity. It begins with the Par-3 homo-oligomerization mechanism and its regulation by Par-1 phosphorylation. The role of polarized cytoskeletal networks in distributing Par-3 clusters to one end of the cell is then discussed, as is the subsequent maintenance of polarized Par-3 clusters through hindered mobility and inhibition from the opposite pole. Finally, specific roles of Par-3 clusters are reviewed, including the bundling of microtubules, the cortical docking of centrosomes, the growth and positioning of cadherin–catenin clusters, and the inhibition of the Par-6–aPKC kinase cassette. Examples are drawn from Drosophila, Caenorhabditis elegans, mammalian cell culture, and biochemical studies.

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