Conjugation of monoclonal antibodies to increase drug delivery efficiency and its specificity

2015 ◽  
pp. 84-105
Author(s):  
Chetan Yewale ◽  
Manisha Lalan ◽  
Ambikanandan Misra
Keyword(s):  
Drug Delivery ◽  
Monoclonal Antibodies ◽  
Delivery Efficiency

Influence of Divalent Cation on Morphology and Drug Delivery Efficiency of Mixed Polymer Nanoparticles

2018 ◽  
Vol 15 (5) ◽  
pp. 652-657 ◽  
Author(s):  
Ramachandran Deepika ◽  
Koyeli Girigoswami ◽  
Ramachandran Murugesan ◽  
Agnishwar Girigoswami
Keyword(s):  
Drug Delivery ◽  
Divalent Cation ◽  
Polymer Nanoparticles ◽  
Delivery Efficiency

scholarly journals Comparison of Salbutamol Delivery Efficiency for Jet versus Mesh Nebulizer Using Mice

Pharmaceutics ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 192 ◽  
Author(s):  
Chang ◽  
Moon ◽  
Oh ◽  
Yoon ◽  
Gu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Aerosol Particles ◽  
Clinical Validation ◽  
Output Rate ◽  
Medication Delivery ◽  
Jet Nebulizer ◽  
Jet Nebulizers ◽  
Effective Medication ◽  
Delivery Efficiency ◽  
Better Than

Recent reports using a breathing simulator system have suggested that mesh nebulizers provide more effective medication delivery than jet nebulizers. In this study, the performances of jet and mesh nebulizers were evaluated by comparing their aerosol drug delivery efficiencies in mice. We compared four home nebulizers: two jet nebulizers (PARI BOY SX with red and blue nozzles), a static mesh nebulizer (NE-U22), and a vibrating mesh nebulizer (NE-SM1). After mice were exposed to salbutamol aerosol, the levels of salbutamol in serum and lung were estimated by ELISA. The residual volume of salbutamol was the largest at 34.6% in PARI BOY SX, while the values for NE-U22 and NE-SM1 mesh nebulizers were each less than 1%. The salbutamol delivery efficiencies of NE-U22 and NE-SM1 were higher than that of PARI BOY SX, as the total delivered amounts of lung and serum were 39.9% and 141.7% as compared to PARI BOY SX, respectively. The delivery efficiency of the mesh nebulizer was better than that of the jet nebulizer. Although the jet nebulizer can generate smaller aerosol particles than the mesh nebulizer used in this study, the output rate of the jet nebulizer is low, resulting in lower salbutamol delivery efficiency. Therefore, clinical validation of the drug delivery efficiency according to nebulizer type is necessary to avoid overdose and reduced drug wastage.

In vivo drug delivery efficiency of albumin-encapsulated liposomes as hydrophobic drug carriers

2018 ◽  
Vol 47 ◽  
pp. 62-66 ◽  
Author(s):  
Yuko Okamoto ◽  
Kazuaki Taguchi ◽  
Mina Sakuragi ◽  
Shuhei Imoto ◽  
Keishi Yamasaki ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Carriers ◽  
Hydrophobic Drug ◽  
Delivery Efficiency

scholarly journals Monoclonal Antibodies Generated against Glycoconjugates Recognize Chemical Linkers

Antibodies ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 48
Author(s):  
Jessica Ramadhin ◽  
Vanessa Silva-Moraes ◽  
Thomas Norberg ◽  
Donald Harn
Keyword(s):  
Drug Delivery ◽  
Flow Cytometry ◽  
Monoclonal Antibodies ◽  
Structure And Function ◽  
Enzyme Linked Immunosorbent Assay ◽  
Incomplete Freund’S Adjuvant ◽  
Delivery Platform ◽  
Igg1 Mabs ◽  
Human Milk Oligosaccharide ◽  
And Function

Monoclonal antibodies (mAbs) that recognize glycans are useful tools to assess carbohydrates’ structure and function. We sought to produce IgG mAbs to the human milk oligosaccharide (HMO), lacto-N-fucopentaose III (LNFPIII). LNFPIII contains the Lewisx antigen, which is found on the surface of schistosome parasites. mAbs binding the Lewisx antigen are well-reported in the literature, but mAbs recognizing HMO structures are rare. To generate mAbs, mice were immunized with LNFPIII-DEX (P3DEX) plus CpGs in VacSIM®, a novel vaccine/drug delivery platform. Mice were boosted with LNFPIII-HSA (P3HSA) plus CpGs in Incomplete Freund’s Adjuvant (IFA). Splenocytes from immunized mice were used to generate hybridomas and were screened against LNFPIII conjugates via enzyme-linked immunosorbent assay (ELISA). Three positive hybridomas were expanded, and one hybridoma, producing IgG and IgM antibodies, was cloned via flow cytometry. Clone F1P2H4D8D5 was selected because it produced IgG1 mAbs, but rescreening unexpectedly showed binding to both LNFPIII and lacto-N-neotetraose (LNnT) conjugates. To further assess the specificity of the mAb, we screened it on two glycan microarrays and found no significant binding. This finding suggests that the mAb binds to the acetylphenylenediamine (APD) linker-spacer structure of the conjugate. We present the results herein, suggesting that our new mAb could be a useful probe for conjugates using similar linker spacer structures.

Biocompatibility and drug delivery efficiency of PEG-b-PCL/hydroxyapatite bilayer coatings on Nitinol superelastic alloy

2020 ◽  
Vol 46 (8) ◽  
pp. 12711-12717
Author(s):  
M.R. Etminanfar ◽  
S.O.R. Sheykholeslami ◽  
V. Khalili ◽  
S. Mahdavi
Keyword(s):  
Drug Delivery ◽  
Delivery Efficiency ◽  
Bilayer Coatings

scholarly journals Therapeutic use of monoclonal antibodies: general aspects and challenges for drug delivery

2017 ◽  
pp. 807-833 ◽  
Author(s):  
Daniela A. Quinteros ◽  
José M. Bermúdez ◽  
Soledad Ravetti ◽  
Alicia Cid ◽  
Daniel A. Allemandi ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Monoclonal Antibodies ◽  
Therapeutic Use ◽  
General Aspects
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