New perspectives in oral peptide and protein delivery: from nanocarrier design to in vivo effectiveness

2016 ◽  
pp. 46-64 ◽  
Author(s):  
María Alonso-Sande ◽  
Noemi Stefania Csaba ◽  
María José Alonso
Keyword(s):  
Protein Delivery

Bifunctional and Bioreducible Dendrimer Bearing a Fluoroalkyl Tail for Efficient Protein Delivery Both In Vitro and In Vivo

Nano Letters ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 8600-8607 ◽  
Author(s):  
Jia Lv ◽  
Changping Wang ◽  
Hongru Li ◽  
Zhan Li ◽  
Qianqian Fan ◽  
...  
Keyword(s):  
Protein Delivery

Multifunctional Click Hyaluronic Acid Nanogels for Targeted Protein Delivery and Effective Cancer Treatment in Vivo

2016 ◽  
Vol 28 (23) ◽  
pp. 8792-8799 ◽  
Author(s):  
Jing Chen ◽  
Yan Zou ◽  
Chao Deng ◽  
Fenghua Meng ◽  
Jian Zhang ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Cancer Treatment ◽  
Protein Delivery

scholarly journals Carboxylated branched poly(β-amino ester) nanoparticles enable robust cytosolic protein delivery and CRISPR-Cas9 gene editing

2019 ◽  
Vol 5 (12) ◽  
pp. eaay3255 ◽  
Author(s):  
Yuan Rui ◽  
David R. Wilson ◽  
John Choi ◽  
Mahita Varanasi ◽  
Katie Sanders ◽  
...  
Keyword(s):  
Protein Delivery ◽  
Gene Editing ◽  
Gene Knockout ◽  
Cell Types ◽  
Endosomal Escape ◽  
Biological Research ◽  
Amino Ester ◽  
Cytosolic Protein

Efficient cytosolic protein delivery is necessary to fully realize the potential of protein therapeutics. Current methods of protein delivery often suffer from low serum tolerance and limited in vivo efficacy. Here, we report the synthesis and validation of a previously unreported class of carboxylated branched poly(β-amino ester)s that can self-assemble into nanoparticles for efficient intracellular delivery of a variety of different proteins. In vitro, nanoparticles enabled rapid cellular uptake, efficient endosomal escape, and functional cytosolic protein release into cells in media containing 10% serum. Moreover, nanoparticles encapsulating CRISPR-Cas9 ribonucleoproteins (RNPs) induced robust levels of gene knock-in (4%) and gene knockout (>75%) in several cell types. A single intracranial administration of nanoparticles delivering a low RNP dose (3.5 pmol) induced robust gene editing in mice bearing engineered orthotopic murine glioma tumors. This self-assembled polymeric nanocarrier system enables a versatile protein delivery and gene editing platform for biological research and therapeutic applications.

scholarly journals A ligand-based system for receptor-specific delivery of proteins

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mariano Maffei ◽  
Chiara Morelli ◽  
Ellie Graham ◽  
Stefano Patriarca ◽  
Laura Donzelli ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Protein Delivery ◽  
Genetic Diseases ◽  
Cell Surface Receptor ◽  
Viral Gene ◽  
Promising Alternative ◽  
Large Proteins ◽  
Reporter Mice ◽  
Surface Receptor

AbstractGene delivery using vector or viral-based methods is often limited by technical and safety barriers. A promising alternative that circumvents these shortcomings is the direct delivery of proteins into cells. Here we introduce a non-viral, ligand-mediated protein delivery system capable of selectively targeting primary skin cells in-vivo. Using orthologous self-labelling tags and chemical cross-linkers, we conjugate large proteins to ligands that bind their natural receptors on the surface of keratinocytes. Targeted CRE-mediated recombination was achieved by delivery of ligand cross-linked CRE protein to the skin of transgenic reporter mice, but was absent in mice lacking the ligand’s cell surface receptor. We further show that ligands mediate the intracellular delivery of Cas9 allowing for CRISPR-mediated gene editing in the skin more efficiently than adeno-associated viral gene delivery. Thus, a ligand-based system enables the effective and receptor-specific delivery of large proteins and may be applied to the treatment of skin-related genetic diseases.

Fluoropolymers for intracellular and in vivo protein delivery

Biomaterials ◽  
2018 ◽  
Vol 182 ◽  
pp. 167-175 ◽  
Author(s):  
Jia Lv ◽  
Bingwei He ◽  
Jingwen Yu ◽  
Yitong Wang ◽  
Changping Wang ◽  
...  
Keyword(s):  
Protein Delivery

Synthesis, protein delivery, and in vitro and in vivo toxicity of a biodegradable poly(aminoester)

2013 ◽  
Vol 2 (6) ◽  
pp. 379 ◽  
Author(s):  
He Shen ◽  
Min Liu ◽  
Yu Chong ◽  
Jie Huang ◽  
Zhijun Zhang
Keyword(s):  
Protein Delivery ◽  
In Vivo Toxicity ◽  
Biodegradable Poly

Microencapsulated chitosan nanoparticles for pulmonary protein delivery: In vivo evaluation of insulin-loaded formulations

2012 ◽  
Vol 157 (3) ◽  
pp. 383-390 ◽  
Author(s):  
S. Al-Qadi ◽  
A. Grenha ◽  
D. Carrión-Recio ◽  
B. Seijo ◽  
C. Remuñán-López
Keyword(s):  
Protein Delivery ◽  
Chitosan Nanoparticles ◽  
In Vivo Evaluation
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