Label-free whole-cell phenotypic diagnostics for cancer

2013 ◽  
pp. 54-67
Author(s):  
Ye Fang
Keyword(s):  
Label Free ◽  
Whole Cell

scholarly journals Label-Free Whole Cell Biosensing for High-Throughput Discovery of Activators and Inhibitors Targeting G Protein-Activated Inwardly Rectifying Potassium Channels

ACS Omega ◽  
2018 ◽  
Vol 3 (11) ◽  
pp. 14814-14823 ◽  
Author(s):  
Katrin M. Krebs ◽  
Eva M. Pfeil ◽  
Katharina Simon ◽  
Manuel Grundmann ◽  
Felix Häberlein ◽  
...  
Keyword(s):  
Potassium Channels ◽  
G Protein ◽  
High Throughput ◽  
Label Free ◽  
Whole Cell ◽  
Inwardly Rectifying Potassium Channels ◽  
Inwardly Rectifying

scholarly journals Constitutive activity of the metabotropic glutamate receptor 2 explored with a whole-cell label-free biosensor

2018 ◽  
Vol 152 ◽  
pp. 201-210 ◽  
Author(s):  
Maarten L.J. Doornbos ◽  
Ilse Van der Linden ◽  
Liesbeth Vereyken ◽  
Gary Tresadern ◽  
Adriaan P. IJzerman ◽  
...  
Keyword(s):  
Glutamate Receptor ◽  
Metabotropic Glutamate Receptor ◽  
Constitutive Activity ◽  
Label Free ◽  
Whole Cell ◽  
Metabotropic Glutamate

scholarly journals A label-free optical whole-cell Escherichia coli biosensor for the detection of pyrethroid insecticide exposure

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Pinpunya Riangrungroj ◽  
Candace Spier Bever ◽  
Bruce D. Hammock ◽  
Karen M. Polizzi
Keyword(s):  
Escherichia Coli ◽  
Pyrethroid Insecticide ◽  
Label Free ◽  
Whole Cell ◽  
Insecticide Exposure

Whole-cell based label-free capacitive biosensor for rapid nanosize-dependent toxicity detection

2015 ◽  
Vol 67 ◽  
pp. 100-106 ◽  
Author(s):  
Anjum Qureshi ◽  
Ashish Pandey ◽  
Raghuraj S. Chouhan ◽  
Yasar Gurbuz ◽  
Javed H. Niazi
Keyword(s):  
Label Free ◽  
Whole Cell ◽  
Capacitive Biosensor

Label-free whole-cell assays: expanding the scope of GPCR screening

2010 ◽  
Vol 15 (17-18) ◽  
pp. 704-716 ◽  
Author(s):  
Clay W Scott ◽  
Matthew F. Peters
Keyword(s):  
Label Free ◽  
Whole Cell ◽  
Cell Assays

scholarly journals Dielectrophoresis as a Tool to Reveal the Potential Role of Ion Channels and Early Electrophysiological Changes in Osteoarthritis

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 949
Author(s):  
Rula Abdallat ◽  
Emily Kruchek ◽  
Csaba Matta ◽  
Rebecca Lewis ◽  
Fatima H. Labeed
Keyword(s):  
Rapid Assessment ◽  
Disease Model ◽  
Membrane Conductance ◽  
Label Free ◽  
Dielectric Parameters ◽  
Whole Cell ◽  
Membrane Conductivity ◽  
Cell Capacitance

Diseases such as osteoarthritis (OA) are commonly characterized at the molecular scale by gene expression and subsequent protein production; likewise, the effects of pharmaceutical interventions are typically characterized by the effects of molecular interactions. However, these phenomena are usually preceded by numerous precursor steps, many of which involve significant ion influx or efflux. As a consequence, rapid assessment of cell electrophysiology could play a significant role in unravelling the mechanisms underlying drug interactions and progression of diseases, such as OA. In this study, we used dielectrophoresis (DEP), a technique that allows rapid, label-free determination of the dielectric parameters to assess the role of potassium ions on the dielectric characteristics of chondrocytes, and to investigate the electrophysiological differences between healthy chondrocytes and those from an in vitro arthritic disease model. Our results showed that DEP was able to detect a significant decrease in membrane conductance (6191 ± 738 vs. 8571 ± 1010 S/m2), membrane capacitance (10.3 ± 1.47 vs. 14.5 ± 0.01 mF/m2), and whole cell capacitance (5.4 ± 0.7 vs. 7.5 ± 0.3 pF) following inhibition of potassium channels using 10 mM tetraethyl ammonium, compared to untreated healthy chondrocytes. Moreover, cells from the OA model had a different response to DEP force in comparison to healthy cells; this was seen in terms of both a decreased membrane conductivity (782 S/m2 vs. 1139 S/m2) and a higher whole cell capacitance (9.58 ± 3.4 vs. 3.7 ± 1.3 pF). The results show that DEP offers a high throughput method, capable of detecting changes in membrane electrophysiological properties and differences between disease states.

scholarly journals Whole cell, label free protein quantitation with data independent acquisition: Quantitation at the MS2 level

PROTEOMICS ◽  
2014 ◽  
Vol 15 (1) ◽  
pp. 16-24 ◽  
Author(s):  
Peter McQueen ◽  
Vic Spicer ◽  
John Schellenberg ◽  
Oleg Krokhin ◽  
Richard Sparling ◽  
...  
Keyword(s):  
Label Free ◽  
Whole Cell ◽  
Free Protein ◽  
Data Independent Acquisition ◽  
Protein Quantitation
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