Label-free monitoring of whole cell vitality

2013 ◽  
Author(s):  
D. Weiss ◽  
M. Brischwein ◽  
H. Grothe ◽  
B. Wolf ◽  
J. Wiest
Keyword(s):  
Label Free ◽  
Whole Cell ◽  
Cell Vitality

scholarly journals Label-Free Whole Cell Biosensing for High-Throughput Discovery of Activators and Inhibitors Targeting G Protein-Activated Inwardly Rectifying Potassium Channels

ACS Omega ◽  
2018 ◽  
Vol 3 (11) ◽  
pp. 14814-14823 ◽  
Author(s):  
Katrin M. Krebs ◽  
Eva M. Pfeil ◽  
Katharina Simon ◽  
Manuel Grundmann ◽  
Felix Häberlein ◽  
...  
Keyword(s):  
Potassium Channels ◽  
G Protein ◽  
High Throughput ◽  
Label Free ◽  
Whole Cell ◽  
Inwardly Rectifying Potassium Channels ◽  
Inwardly Rectifying

scholarly journals Cellular effects of gliotoxin: Evaluation of a proteomic, isotope-based method to detect reactive cysteines

2021 ◽  
Author(s):  
◽  
Sven Sondhauss
Keyword(s):  
Large Scale ◽  
Thiol Group ◽  
Disulfide Bridge ◽  
Reversed Phase ◽  
Cellular Damage ◽  
Full Potential ◽  
Label Free ◽  
Cysteine Modification ◽  
Affinity Tag ◽  
Cell Vitality

<p>Cysteinyl residues in proteins are important for many cellular processes and unregulated modification of the cysteine thiol group can have negative effects on cell vitality and viability. In this thesis, the potential for use of the isotope coded affinity tag (ICAT) method for detection of cysteine modification has been investigated. ICAT reagents label free cysteine thiols. The aim of this study was to use HL-60 cells treated with gliotoxin, a fungal metabolite with a reactive disulfide bridge, as a system to evaluate the performance of ICAT for identification of cysteine modification in a whole cell proteome. Gliotoxin has antimicrobial, antitumor, immunosuppressive and cytotoxic properties that have been related to cysteine modification in proteins. Cellular assays including viability using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, cell cycle analysis, and measurement of reactive oxygen species using dichlorofluorescin diacetate were used to establish conditions for measuring the effects of gliotoxin on HL-60 cells prior to large-scale cellular damage. Cells exposed to gliotoxin and control cells were then labeled with ICAT reagents and analysed by offline reversed phase liquid chromatography followed by matrix-assisted laser desorption/ionization tandem mass spectrometry. The pilot results identified tubulin, glyceraldehyde-3-phosphate dehydrogenase and peptidyl-prolyl cis-trans isomerase as putative targets of gliotoxin. Additionally, this study showed that ICAT can be used to detect modified cysteines from a highly complex sample, but further optimization is needed to unlock the full potential for detection of cysteine modification in complex samples.</p>

scholarly journals Constitutive activity of the metabotropic glutamate receptor 2 explored with a whole-cell label-free biosensor

2018 ◽  
Vol 152 ◽  
pp. 201-210 ◽  
Author(s):  
Maarten L.J. Doornbos ◽  
Ilse Van der Linden ◽  
Liesbeth Vereyken ◽  
Gary Tresadern ◽  
Adriaan P. IJzerman ◽  
...  
Keyword(s):  
Glutamate Receptor ◽  
Metabotropic Glutamate Receptor ◽  
Constitutive Activity ◽  
Label Free ◽  
Whole Cell ◽  
Metabotropic Glutamate

scholarly journals A label-free optical whole-cell Escherichia coli biosensor for the detection of pyrethroid insecticide exposure

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Pinpunya Riangrungroj ◽  
Candace Spier Bever ◽  
Bruce D. Hammock ◽  
Karen M. Polizzi
Keyword(s):  
Escherichia Coli ◽  
Pyrethroid Insecticide ◽  
Label Free ◽  
Whole Cell ◽  
Insecticide Exposure

Whole-cell based label-free capacitive biosensor for rapid nanosize-dependent toxicity detection

2015 ◽  
Vol 67 ◽  
pp. 100-106 ◽  
Author(s):  
Anjum Qureshi ◽  
Ashish Pandey ◽  
Raghuraj S. Chouhan ◽  
Yasar Gurbuz ◽  
Javed H. Niazi
Keyword(s):  
Label Free ◽  
Whole Cell ◽  
Capacitive Biosensor

scholarly journals MULTISENSORS FOR WHOLE-CELL ANALYTICS

2018 ◽  
Vol 8 (2) ◽  
pp. 39-41 ◽  
Author(s):  
Ingo Tobehn-Steinhäuser ◽  
Margarita Günther ◽  
Stefan Görlandt ◽  
Steffen Herbst ◽  
Heike Wünscher ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Yeast Cells ◽  
Signal Molecules ◽  
Fluorescence Signal ◽  
Specific Stimulus ◽  
Whole Cell ◽  
Biochemical Processes ◽  
Cell Vitality ◽  
On Line

Whole-cell biosensors, which can be used in the environmental protection and process measuring technology, in the biotechnology, food and pharmaceutical industry for the on-line control and monitoring of chemical and biochemical processes and for the fast detection of small amounts of solutes, are of great interest. These kind of biosensors comprise microorganisms like genetically modified yeast cells, which react on a specific stimulus, e.g. a residual amount of drugs in the measurement medium with a dependent fluorescence. The receptors of the cells detect specific signal molecules and stimulate the organism to produce a fluorescent protein. In this work, a combined impedimetric-optical read out was used, which can provide the reliable and long-term stable detection of a fluorescence signal accompanied by impedance measurements monitoring cell vitality and activity. A new challenge thereby is a special sensor design which enables the integration of excitation source, photodetector, interdigitated electrodes, and the fluidic system with an effective and long-term-stable packaging.

Label-free whole-cell assays: expanding the scope of GPCR screening

2010 ◽  
Vol 15 (17-18) ◽  
pp. 704-716 ◽  
Author(s):  
Clay W Scott ◽  
Matthew F. Peters
Keyword(s):  
Label Free ◽  
Whole Cell ◽  
Cell Assays
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