Matrix interference from Fc–Fc interactions in immunoassays for detecting human IgG4 therapeutics

Bioanalysis ◽  
2015 ◽  
Vol 7 (20) ◽  
pp. 2701-2712 ◽  
Author(s):  
Michael A Partridge ◽  
Elif Kabuloglu Karayusuf ◽  
Gangadhar Dhulipala ◽  
Robert Dreyer ◽  
Thomas Daly ◽  
...  
Keyword(s):  
Matrix Interference

scholarly journals High-Efficiency, Matrix Interference-Free, General Applicable Probes for Bile Acids Extraction and Detection

2018 ◽  
Vol 5 (12) ◽  
pp. 1800774 ◽  
Author(s):  
Shuyao Huang ◽  
Jiating Zheng ◽  
Qian Yang ◽  
Guosheng Chen ◽  
Jianqiao Xu ◽  
...  
Keyword(s):  
Bile Acids ◽  
High Efficiency ◽  
Matrix Interference

scholarly journals Fate of selected pharmaceuticals and personal care products after secondary wastewater treatment processes in Taiwan

2010 ◽  
Vol 62 (10) ◽  
pp. 2450-2458 ◽  
Author(s):  
Angela Yu-Chen Lin ◽  
Cheng-Fan Lin ◽  
Yu-Ting Tsai ◽  
Hank Hui-Hsiang Lin ◽  
Jie Chen ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
High Performance ◽  
Wastewater Treatment Plants ◽  
Solid Phase ◽  
Personal Care Products ◽  
Personal Care ◽  
Matrix Interference ◽  
Treatment Processes ◽  
Liquid Chromatography Tandem Mass ◽  
Removal Efficiencies

Pharmaceuticals and personal care products (PPCPs) constitute a class of chemicals of emerging concern due to the potential risks they pose to organisms and the environment, even at low concentrations (ng/L). Recent studies have found that PPCPs are not efficiently removed in secondary wastewater treatment plants (WWTPs). This study has: (1) simultaneously investigated the occurrence of sixty-one PPCPs using solid phase extraction and high-performance liquid chromatography-tandem mass spectrometry, (2) evaluated removal efficiencies of target PPCPs in six WWTPs that discharge effluents into major Taiwanese rivers, and lastly (3) examined matrix interference during analysis of target PPCPs in water samples. The twenty target PPCPs were chosen for their high detection frequencies, high influent concentrations, and stability during wastewater treatment processes. Caffeine and acetaminophen were detected at the highest concentrations (as high as 24,467 and 33,400 ng/L) and were effectively removed (both >96%); other PPCPs were detected in the high ng/L range but were not effectively removed. Matrix interference (by ion suppression or enhancement) during the analysis resulted in underestimation of the removal efficiencies of erythromycin-H2O, cefazolin, clarithromycin, ibuprofen, diclofenac, clofibric acid and gemfibrozil.

Trace element determination in high-salinity water using an online ion imprinted helical tube demineralizer combined with ICP-MS

2020 ◽  
Vol 35 (7) ◽  
pp. 1306-1315
Author(s):  
Hongyan Li ◽  
Bei Zheng ◽  
Chunfu Li ◽  
Jiale Zheng ◽  
Yanfen Zhang
Keyword(s):  
High Salinity ◽  
Matrix Interference ◽  
Element Determination ◽  
High Salinity Water ◽  
Icp Ms ◽  
Ion Imprinted ◽  
Helical Tube ◽  
Trace Elements In Water ◽  
Salinity Water ◽  
P Matrix

Matrix interference is a common problem in the measurement of trace elements in water via ICP-MS.

scholarly journals Identification and elimination of target-related matrix interference in a neutralizing anti-drug antibody assay

2014 ◽  
Vol 403 (1-2) ◽  
pp. 52-61 ◽  
Author(s):  
Martin Schwickart ◽  
Freshta Mehrzai ◽  
Jennifer Pearson ◽  
Nabila Shaghasi ◽  
Carlos Chavez ◽  
...  
Keyword(s):  
Matrix Interference ◽  
Antibody Assay

Surrogate-Assisted Determination of 2,3,7,8-Tetrachlorodibenzo-p-Dioxin in Fish by Electron Capture Capillary Gas Chromatography

1986 ◽  
Vol 69 (6) ◽  
pp. 976-980
Author(s):  
Richard A Niemann
Keyword(s):  
Gas Chromatography ◽  
Electron Capture ◽  
Capillary Gas Chromatography ◽  
Capillary Column ◽  
Electron Capture Detection ◽  
Matrix Interference ◽  
Relative Standard ◽  
Tetrachlorodibenzo P Dioxin ◽  
Liquid Chromatographic

Abstract Surrogate spiking the sample with 1000 parts per trillion (pptr) 1,3,7,8-tetrachlorodibenzo-p-dioxin (1378-TCDD) has doubled analytical throughput in determining toxic 2378-TCDD (analyte) at the low partper- trillion level in fish, using multicolumn high resolution liquid chromatographic cleanup before quantitation by capillary gas chromatography with electron capture detection. The 1378- and 2378-TCDD were recovered equally and were well separated by the capillary column so that the earlier-eluting surrogate did not interfere with the quantitation of levels of analyte many-fold lower. Matrix interference contributed <1 % bias in surrogate quantitation. Using surrogate recovery to correct for analyte losses during analysis, accuracy averaged (n = 7) 105% in determining 18 or 45 pptr 2378-TCDD added to fish without detectable bioincurred analyte. Analyses of selected fish with bioincurred 2378-TCDD gave results comparable to earlier work where recovery correction required a second analysis of sample fortified with analyte. With surrogate fortification, repeatability of determination (n = 3 or 4) improved markedly to <5% relative standard deviation at 37-46 pptr.

Detection of trace amounts of abamectin used as an antiparasitic agent in fallow deer tissues

2009 ◽  
Vol 57 (1) ◽  
pp. 99-107 ◽  
Author(s):  
Diana Žele ◽  
Silvestra Kobal ◽  
Gorazd Vengušt ◽  
Andrej Bidovec ◽  
Anton Vengušt ◽  
...  
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Fallow Deer ◽  
Reversed Phase ◽  
Fat Tissue ◽  
Matrix Interference ◽  
Solid Phase Extraction Cartridge ◽  
Tissue Matrix

A sensitive and reliable method for the determination of trace amounts of abamectin in muscles, kidneys and fat tissue of fallow deer is presented. Abamectin was extracted from the tissues with acetonitrile and the extract was cleaned up on a C8 solid-phase extraction cartridge. Abamectin residue was derivatised with trifluoroacetic acid anhydride and 1-methylimidazole, and determined using reversed- phase high-performance liquid chromatography under isocratic conditions and fluorescence detection. The recoveries of the method were high and consistent, ranging from 78% to 90%. The limit of detection of the method was below 1 μg/kg when analysing muscle, kidney and fat tissue. Matrix-matched calibration was used in order to obtain accurate values and to avoid matrix interference.

scholarly journals Metabolite and matrix interference in phenytoin immunoassays

1996 ◽  
Vol 42 (10) ◽  
pp. 1645-1653 ◽  
Author(s):  
P M Rainey ◽  
K E Rogers ◽  
W L Roberts
Keyword(s):  
Matrix Effect ◽  
Fluorescence Polarization ◽  
Cross Reactivity ◽  
Positive Bias ◽  
Fluorescence Polarization Immunoassay ◽  
Matrix Interference ◽  
Clinical Specimens ◽  
Significant Bias ◽  
Clinically Significant ◽  
Minimum Detection Limits

Abstract The major phenytoin metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin glucuronide (HPPG), was primarily responsible for the positive bias noted when uremic specimens were assayed with the Abbott TDx Free Phenytoin fluorescence polarization immunoassay. The amount of bias depended on both HPPG and phenytoin concentration, increasing with increases in either concentration. The new Abbott TDx II assays for phenytoin and free phenytoin exhibited no significant cross-reactivity with HPPG and no bias in clinical specimens from uremic patients. Both assays correlated well with Emit-based assays (r >0.98), had CVs of <3.5%, and had minimum detection limits of <0.1 mg/L. Calibration curves were stable for at least 6 weeks. All of the TDx assays cross-reacted with another metabolite, 5-(p-hydroxyphenyl)-5-phenylhydantoin (HPPH), but expected HPPH concentrations are too low to cause a clinically significant bias. The Emit-based phenytoin assay exhibited a significant matrix effect when calibrators were prepared in defibrinated plasma processed to resemble serum.

scholarly journals Multiresidue Method for Determination of Organochlorine Insecticides and Polychlorinated Biphenyl Congeners in Fatty Processed Foods

1996 ◽  
Vol 79 (6) ◽  
pp. 1434-1446 ◽  
Author(s):  
Anna Sannino ◽  
Paola Mambriani ◽  
Mirella Bandini ◽  
Luciana Bolzoni
Keyword(s):  
Polychlorinated Biphenyl ◽  
Methylene Chloride ◽  
Gas Chromatography Mass Spectrometry ◽  
Animal Origin ◽  
Electron Capture Detection ◽  
Matrix Interference ◽  
Selected Ion Monitoring ◽  
Pcb Congeners ◽  
Organochlorine Insecticides

Abstract A previously published gel permeation chromatographic (GPC) procedure for determination of organophosphorous insecticides is used to determine 24 organochlorine (OC) pesticide and 9 polychlorinated biphenyl (PCB) residues in 8 fatty preserved foods of vegetable and animal origin. Samples are extracted with methylene chloride and cleaned up by automated GPC with a Biobeads SX3 column and a methylene chloride–cyclohexane (15 + 85) eluant. Compounds are quantitated by gas chromatography/mass spectrometry (GC/MS) with selected ion monitoring using a DB-5 column. Average recoveries of OC pesticides from samples fortified at 0.010–0.080 mg/kg ranged from 73 to 98%. Average recoveries of 9 PCB congeners spiked at 2 levels (0.0025 and 0.020 mg/kg) were between 85 and 104%. Quantitation limits were between 0.002 and 0.025 mg/kg, depending on the compound. The purified extracts were analyzed further by GC with electron capture detection (ECD), and the results were compared with those obtained by MS. Determination of some OC pesticides and PCB congeners was not possible by ECD because of matrix interference.

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