scholarly journals Effect of blood volume on analytical bias in dried blood spots prepared for newborn screening external quality assurance

Bioanalysis ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 99-109 ◽  
Author(s):  
Stuart J Moat ◽  
Catherine Dibden ◽  
Lesley Tetlow ◽  
Caroline Griffith ◽  
Jim Chilcott ◽  
...  
Keyword(s):  
Quality Assurance ◽  
Newborn Screening ◽  
Blood Volume ◽  
Filter Paper ◽  
Whole Blood ◽  
Dried Blood Spots ◽  
Measurement Bias ◽  
External Quality ◽  
Blood Spots ◽  
The Uk

Aim: Dried blood spots (DBS) are used for the analysis of more than 2000 biomarkers. We assessed a range of analyte concentrations and diameters of DBS. Materials & methods: DBS samples were created by the application of increasing volumes of whole blood prepared by the UK NEQAS Quality Assurance Laboratory. Samples were analyzed in four separate laboratories. Results: Volumes less than 25 μl (8 mm) and more than 75 μl (14 mm) created unsatisfactory analytical biases. Results obtained from peripheral subpunches tended to be higher than those from a central subpunch. Conclusion: DBS diameters formed from nonvolumetric application of blood to filter paper can be used to assess whether measurement bias will be within acceptable limits according to the analyte being quantified. DBS received for newborn screening in the UK with diameters less than 8 mm and those more than 14 mm should be rejected.

Gaucher and Niemann–Pick diseases—enzymatic diagnosis in dried blood spots on filter paper: retrospective diagnoses in newborn-screening cards

2002 ◽  
Vol 317 (1-2) ◽  
pp. 191-197 ◽  
Author(s):  
Néstor A. Chamoles ◽  
Mariana Blanco ◽  
Daniela Gaggioli ◽  
Carina Casentini
Keyword(s):  
Newborn Screening ◽  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Niemann Pick

DNA microextraction from dried blood spots on filter paper blotters: potential applications to newborn screening

1987 ◽  
Vol 75 (3) ◽  
pp. 213-216 ◽  
Author(s):  
Edward R. B. McCabe ◽  
Shu-Zhen Huang ◽  
William K. Seltzer ◽  
Martha L. Law
Keyword(s):  
Newborn Screening ◽  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Potential Applications

scholarly journals Effect of Dried Blood Spot Quality on Newborn Screening Analyte Concentrations and Recommendations for Minimum Acceptance Criteria for Sample Analysis

2016 ◽  
Vol 62 (3) ◽  
pp. 466-475 ◽  
Author(s):  
Roanna S George ◽  
Stuart J Moat
Keyword(s):  
Newborn Screening ◽  
Filter Paper ◽  
Statistical Significance ◽  
False Negative ◽  
Dried Blood Spots ◽  
Sample Analysis ◽  
Acceptance Criteria ◽  
Blood Spots ◽  
Spot Quality ◽  
Blood Spot

Abstract BACKGROUND The analysis of dried blood spots has been used routinely for newborn screening since the early 1970s, and the number of disorders screened has expanded substantially in recent years. However, there is a lack of evidence regarding minimum blood spot quality acceptance criteria for sample analysis. METHODS Blood pools were spiked with phenylalanine, tyrosine, leucine, methionine, octanoylcarnitine, decanoylcarnitine, isovalerylcarnitine, glutarylcarnitine, thyroid-stimulating hormone, and immunoreactive trypsinogen to concentrations at the analytical cutoffs used in UK screening protocols. We evaluated the effect of sample volume applied to the card (10, 20, 50, 75, and 100 μL), punch location (central vs peripheral), and sample quality (double layering, applying blood to both sides of the filter paper, multispotting, applying insufficient sample, and compressing the sample after application). RESULTS Compression of blood spots produced significantly lower results (14%–44%) for all analytes measured (P < 0.001). Smaller blood spots produced significantly lower results (15%–24% for 10-μL vs 50-μL sample size) for all analytes at all concentrations measured (P < 0.001). Results obtained from peripheral punches were higher than those from a central punch, although this did not reach statistical significance for all analytes. Insufficient and multispotted samples demonstrated heterogeneous results. CONCLUSIONS All blood spots containing ≤20 μL (blood spot diameter <8 mm), those in which blood has not fully penetrated the filter paper, and all samples with evidence of compression should be rejected, since there is a risk of producing false-negative results.

scholarly journals The development and validation of dried blood spots for external quality assurance of syphilis serology

2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Pieter W Smit ◽  
Thomas van der Vlis ◽  
David Mabey ◽  
John Changalucha ◽  
Julius Mngara ◽  
...  
Keyword(s):  
Quality Assurance ◽  
Dried Blood Spots ◽  
External Quality Assurance ◽  
External Quality ◽  
Blood Spots ◽  
Development And Validation ◽  
Syphilis Serology

Phenylalanine analyses of blood-spot control materials: preparation of samples and evaluation of interlaboratory performance.

1985 ◽  
Vol 31 (2) ◽  
pp. 235-238 ◽  
Author(s):  
F W Spierto ◽  
T L Hearn ◽  
F H Gardner ◽  
W H Hannon
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Filter Paper ◽  
Whole Blood ◽  
High Performance ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Interlaboratory Studies ◽  
Blood Spot ◽  
Materials Preparation

Abstract Aliquots (0.1 mL) of whole-blood pools prepared to contain various concentrations of phenylalanine were applied to filter-paper collection cards, dried, and stored in sealed bags. We measured the phenylalanine content of the dried blood spots by bioassay, fluorometry, and "high-performance" liquid chromatography, and found that the concentrations remained constant for two years when samples were kept at -20 degrees C or lower. Intra- and interlaboratory studies showed that results for phenylalanine were greater for laboratories using bioassay procedures than for those using fluorometric procedures. Further, CVs (both among- and within-laboratory) obtained with fluorometric procedures were nearly half as great as the CVs obtained by laboratories using bioassay techniques.

Tay-Sachs and Sandhoff diseases: enzymatic diagnosis in dried blood spots on filter paper: retrospective diagnoses in newborn-screening cards

2002 ◽  
Vol 318 (1-2) ◽  
pp. 133-137 ◽  
Author(s):  
Néstor A Chamoles ◽  
Mariana Blanco ◽  
Daniela Gaggioli ◽  
Carina Casentini
Keyword(s):  
Newborn Screening ◽  
Filter Paper ◽  
Dried Blood Spots ◽  
Blood Spots

scholarly journals Gender identification in Chicken (Gallus gallus) by PCR using whole blood and dried blood spot on filter paper as template: without prior DNA isolation

2016 ◽  
Author(s):  
S. Dhanasekaran ◽  
G. Dhinakar Raj ◽  
A. R. Vignesh ◽  
S. T. Selvan ◽  
B. Prakash ◽  
...  
Keyword(s):  
Sex Determination ◽  
Dna Extraction ◽  
Filter Paper ◽  
Whole Blood ◽  
Dried Blood Spots ◽  
Sex Identification ◽  
Gender Identification ◽  
Blood Samples ◽  
Blood Spots ◽  
Blood Spot

AbstractAccurate sex identification of pure line chickens in their early age has significant economic impact in breeding industry. In the recent years, range of Polymerase Chain Reaction (PCR) based sex determination techniques are routinely used to identify the sex of parent lines in breeding industries, however purified DNA is a prerequisite. Hence this study was aimed to develop a rapid and inexpensive PCR based gender identification method for chicken using whole blood samples and dried blood spots as template for PCR without DNA extraction. In addition, practicability of two W-chromosome specific gene targets in chicken for sex determination also characterised. Successful amplification of sex specific fragments and an internal control was achieved with the range of 0.125μl and 0.250μl volume of whole blood on filter paper (~1 mm) prepared from chicken and dried blood spot. This technique does not require DNA extraction, freeze/thawing of blood samples, pre-treatment with any reagents, dilution of whole blood or dried blood spots on filter paper. It can be carried out with commercially available Taq polymerase enzymes with increased concentration of MgCl2 (3 mM) and 0.5% of DMSO without optimisation of PCR buffers. In conclusion, as compared to the existing PCR based sex identification techniques, the present approach is relatively economic, time saving, requires minimal steps and eliminates the need for DNA extraction.

scholarly journals Utility of measuring very long-chain fatty-acyl carnitines in dried blood spots for newborn screening of X-linked Adrenoleukodystrophy

2021 ◽  
Vol 26 ◽  
pp. 100720
Author(s):  
Archana Natarajan ◽  
Rita Christopher ◽  
Shruti V. Palakuzhiyil ◽  
Sadanandavalli Retnaswami Chandra
Keyword(s):  
Newborn Screening ◽  
Dried Blood Spots ◽  
Fatty Acyl ◽  
Blood Spots ◽  
Long Chain

Alpha- l -iduronidase and arylsulfatase B in dried blood spots on filter paper: Biochemical parameters and time stability

2017 ◽  
Vol 50 (7-8) ◽  
pp. 431-435 ◽  
Author(s):  
Ana Carolina Breier ◽  
Jaqueline Cé ◽  
Jamila Mezzalira ◽  
Vanessa V. Daitx ◽  
Vitoria C. Moraes ◽  
...  
Keyword(s):  
Filter Paper ◽  
Biochemical Parameters ◽  
Dried Blood Spots ◽  
Time Stability ◽  
Blood Spots ◽  
Arylsulfatase B

PCR of DNA from dried blood spots on filter paper coupled to a simple DNA enzyme immunoassay for rapid detection of HIV-1

1996 ◽  
Vol 52 (4) ◽  
pp. 308-309
Author(s):  
B. Schweiger ◽  
C. Kücherer ◽  
C. Fleischer ◽  
H. v. Spreckelsen ◽  
P. Zablocki-Kaiser ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Filter Paper ◽  
Rapid Detection ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Dna Enzyme Immunoassay ◽  
Hiv 1
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